With implications in several medical conditions, N‐linked glycosylation is one of the most important posttranslation modifications present in all living organisms. Due to their nontemplate synthesis, glycan structures are extraordinarily complex and require multiple analytical techniques for complete structural elucidation. Mass spectrometry is the most common way to investigate N‐linked glycans; however, with techniques such as liquid‐chromatography mass spectrometry, there is complete loss of spatial information. Mass spectrometry imaging is a transformative analytical technique that can visualize the spatial distribution of ions within a biological sample and has been shown to be a powerful tool to investigate N‐linked glycosylation. This review covers the fundamentals of mass spectrometry imaging and N‐linked glycosylation and highlights important findings of recent key studies aimed at expanding and improving the glycomics imaging field.