Complex-type<i>N</i>-glycans on VSV-G pseudotyped HIV exhibit ‘tough’ sialic and ‘brittle’ mannose self-adhesions

Abeyratne-Perera, Hashanthi K.; Ogharandukun, Eric; Chandran, Preethi L.

doi:10.1039/c9sm00579j

Cited by 8 publications

(2 citation statements)

References 77 publications

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“…Cationic polymers, such as polybrene, which have been commonly utilized for LV experiments, increase viral transduction efficiency by counteracting repulsive electrostatic effects [ 56 ]. The VSV-G pseudotyped LV is covered with an N-glycosylation “shield” and its terminal sialic acids tightly adhere to each other promoting viral self-aggregation in the presence of Ca 2+ ions [ 57 , 58 ]. The VSV-G pseudotyped LV treated with neuraminidase, which cleaves terminal sialic acid, showed increased infectivity compared with normal VSV-G [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

The Dual-Pseudotyped Lentiviral Vector with VSV-G and Sendai Virus HN Enhances Infection Efficiency through the Synergistic Effect of the Envelope Proteins

Jargalsaikhan,

Muto,

Been

et al. 2024

Viruses

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A gene delivery system utilizing lentiviral vectors (LVs) requires high transduction efficiency for successful application in human gene therapy. Pseudotyping allows viral tropism to be expanded, widening the usage of LVs. While vesicular stomatitis virus G (VSV-G) single-pseudotyped LVs are commonly used, dual-pseudotyping is less frequently employed because of its increased complexity. In this study, we examined the potential of phenotypically mixed heterologous dual-pseudotyped LVs with VSV-G and Sendai virus hemagglutinin-neuraminidase (SeV-HN) glycoproteins, termed V/HN-LV. Our findings demonstrated the significantly improved transduction efficiency of V/HN-LV in various cell lines of mice, cynomolgus monkeys, and humans compared with LV pseudotyped with VSV-G alone. Notably, V/HN-LV showed higher transduction efficiency in human cells, including hematopoietic stem cells. The efficient incorporation of wild-type SeV-HN into V/HN-LV depended on VSV-G. SeV-HN removed sialic acid from VSV-G, and the desialylation of VSV-G increased V/HN-LV infectivity. Furthermore, V/HN-LV acquired the ability to recognize sialic acid, particularly N-acetylneuraminic acid on the host cell, enhancing LV infectivity. Overall, VSV-G and SeV-HN synergistically improve LV transduction efficiency and broaden its tropism, indicating their potential use in gene delivery.

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Section: Discussionmentioning

confidence: 99%

The Dual-Pseudotyped Lentiviral Vector with VSV-G and Sendai Virus HN Enhances Infection Efficiency through the Synergistic Effect of the Envelope Proteins

Jargalsaikhan,

Muto,

Been

et al. 2024

Viruses

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“…For example, heavily glycosylated proteins are abundant in negatively charged side chains containing molecules such as sulfate, phosphate, and especially sialic acid in O-glycosylated proteins, which could confer repulsive forces that would hinder clustering. One way to compensate for these factors is the presence of divalent cations like Ca 2+ that may allow charge-based attractions of acidic glycan chains [111,113,114]. Another potential mechanism, on the other hand, may be through regulation of the protonation state of glycans and glycolipids.…”

Section: Luminal Acidification Is Key For Apical Sorting and Traffickingmentioning

confidence: 99%

Self‐organization of apical membrane protein sorting in epithelial cells

Levic

Bagnat

2021

The FEBS Journal

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Polarized epithelial cells are characterized by the asymmetric distribution of proteins between apical and basolateral domains of the plasma membrane. This asymmetry is highly conserved and is fundamental to epithelial cell physiology, development, and homeostasis. How proteins are segregated for apical or basolateral delivery, a process known as sorting, has been the subject of considerable investigation for decades. Despite these efforts, the rules guiding apical sorting are poorly understood and remain controversial. Here, we consider mechanisms of apical membrane protein sorting and argue that they are largely driven by self‐organization and biophysical principles. The preponderance of data to date is consistent with the idea that apical sorting is not ruled by a dedicated protein‐based sorting machinery and relies instead on the concerted effects of oligomerization, phase separation of lipids and proteins in membranes, and pH‐dependent glycan interactions.

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Shells of compacted DNA as nanocontainers transporting proteins in multiplexed delivery

Abeyratne-Perera

Basu

Chandran

2021

Materials Science and Engineering: C

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Complex-typeN-glycans on VSV-G pseudotyped HIV exhibit ‘tough’ sialic and ‘brittle’ mannose self-adhesions

Abstract: Mannose and sialic acid residues exhibit short-range brittle self-adhesion and long-range tough self-adhesion in both monolayers and complex type N-glycans.

Cited by 8 publications

References 77 publications

The Dual-Pseudotyped Lentiviral Vector with VSV-G and Sendai Virus HN Enhances Infection Efficiency through the Synergistic Effect of the Envelope Proteins

The Dual-Pseudotyped Lentiviral Vector with VSV-G and Sendai Virus HN Enhances Infection Efficiency through the Synergistic Effect of the Envelope Proteins

Self‐organization of apical membrane protein sorting in epithelial cells

Shells of compacted DNA as nanocontainers transporting proteins in multiplexed delivery

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