Complex viscoelasticity of normal and lectin treated erythrocytes using laser diffractometry

A dynamic and stationary rheological study of erythrocytes incubated in a glucose medium

“…These parameters can be satisfactorily used to describe the viscoelastic characteristics of RBC membranes as is shown in the papers of Chmiel et al [12] and Riquelme et al [1,9]. In this case, r 0 /c 0 is the stationary elastic modulus of the erythrocyte membrane, l.…”

“…In the stationary mode [8], the lower rotating disk rotates at constant speed. In the oscillating mode [9], the lower disk moves at sinusoidal oscillating speeds at six pre-established frequencies (0.5, 1, 1.5, 2, 2.5 and 3 Hz). A laser beam traverses perpendicularly the suspension of sheared erythrocytes, producing an elliptical diffraction pattern.…”

“…Under dynamic regime (oscillating mode), the lower disk of the Erythrodeformeter rotates at sinusoidal variable speed [9]. Oscillation amplitude and frequency are electronically controlled a set of six frequencies: 0.5, 1, 1.5, 2, 2.5 and 3 Hz have been preset.…”

Impairment of Erythrocytes Incubated in Glucose Medium: A Wavelet-Information Theory Analysis

“…The wavelet transform allow us an useful characterization of the experimental signal (time series) by the amplitude distribution of the coefficients in the wavelet basis [25,26]. The j-scale wavelet coefficients family set {C j (k)} could be interpreted as the local residual errors between successive signal approximations at levels j and j -1 [18]. It contains information on the signal S(t) corresponding to the frequencies 2 ðjÀ1Þ w s w j j 2 j w s ; where w s represent the sample frequency.…”

“…Time series were obtained from the longitudinal axe fluctuations from the elliptical diffraction pattern corresponding to erythrocytes subjected to shear stress measured with the 'Erythrodeformeter', which has been completely described in previous studies [17][18][19][20][21]. In this device, the RBC suspension is sheared between two parallel concentric discs, an upper fixed disc and a lower rotating one.…”