2021
DOI: 10.3390/ijms222111666
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Complexation of CXCL12, FGF-2 and VEGF with Heparin Modulates the Protein Release from Alginate Microbeads

Abstract: Long-term delivery of growth factors and immunomodulatory agents is highly required to support the integrity of tissue in engineering constructs, e.g., formation of vasculature, and to minimize immune response in a recipient. However, for proteins with a net positive charge at the physiological pH, controlled delivery from negatively charged alginate (Alg) platforms is challenging due to electrostatic interactions that can hamper the protein release. In order to regulate such interactions between proteins and … Show more

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Cited by 7 publications
(6 citation statements)
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“…As demand increases, safe and effective facial fillers are being updated, such as biodegradable calcium hydroxyapatite, polycaprolactone, hyaluronic acid, and non‐biodegradable poly‐meth methacrylate silicone, polyacrylamide, and autologous fat grafting 6,7 . Poly‐L‐Lactic Acid (PLLA) is a biocompatible, biodegradable, absorbable, immunologically inert, malleable, and easy‐to‐process polymeric synthetic polymer derived from plants that have been used in previous studies of synthetic suture materials.…”
Section: Introductionmentioning
confidence: 99%
“…As demand increases, safe and effective facial fillers are being updated, such as biodegradable calcium hydroxyapatite, polycaprolactone, hyaluronic acid, and non‐biodegradable poly‐meth methacrylate silicone, polyacrylamide, and autologous fat grafting 6,7 . Poly‐L‐Lactic Acid (PLLA) is a biocompatible, biodegradable, absorbable, immunologically inert, malleable, and easy‐to‐process polymeric synthetic polymer derived from plants that have been used in previous studies of synthetic suture materials.…”
Section: Introductionmentioning
confidence: 99%
“…To evaluate protein binding to HTCC, we used Lz as a model protein (because its molecular weight and isoelectric point (pI) are close to those of CXCL12, FGF-2, TGF-β1, and VEGF; Table S3) to follow the interactions between the pure protein or Hep–protein complex and HTCC. We recently determined K a = 2.3 × 10 6 M –1 for the binding of Hep to Lz, which is similar to the binding of Hep to HTCC. The small quantities of Lz in the Hep–Lz complex resulted in minor changes in the titration isotherms (Figure S4), and the thermodynamic parameters for binding of Hep–Lz to HTCC are almost identical to those for pure Hep.…”
Section: Resultsmentioning
confidence: 99%
“…Therefore, in addition to the potential effects on protein release and shortening the assembly process, protein deposition using a protein cosolution with Hep is expected to preserve the protein bioactivity during the long-term coating process. Moreover, growth factors released from delivery systems as complexes with Hep have been found to influence the cellular response more effectively than the proteins alone . Furthermore, an anchoring bilayer consisting of HTCC and tannic acid (TA), also known to physically crosslink macromolecules at multiple binding sites through multiple interactions, including hydrogen bonding, ionic bonding, and π–π/cation−π interactions, was determined to increase the film stability during the film buildup.…”
Section: Introductionmentioning
confidence: 99%
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“…Furthermore, they have targeting efficiency, sustainability, and controllable release [ 17 , 18 , 19 ]. During the formulation of alginate-based microbeads, the active substance is entrapped in a gel of alginate that is crosslinked with divalent cations such as Ca 2+ , Ba 2+ , and Sr 2+ , with uronic acid residues in alginate [ 20 , 21 , 22 , 23 ].…”
Section: Introductionmentioning
confidence: 99%