Complexation of Hg with phytochelatins is important for plant Hg tolerance

Carrasco-Gil, Sandra; Álvarez‐Fernández, Ana; Sobrino-Plata, Juan; Millán, Rocío; Carpena‐Ruiz, Ramón O.; LeDuc, Danika L.; Andrews, Joy C.; Abadı́a, Javier; Hernández, Luís E.

doi:10.1111/j.1365-3040.2011.02281.x

Cited by 110 publications

(105 citation statements)

References 58 publications

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“…An increase in GR activity was also noticed in Phaseolus vulgaris and Alyssum following exposure to Cd (Chaoui et al 1997). Higher synthesis of PCs for Pb alone or in combination to EDTA was also reported earlier in Vallisneria spiralis, Hydrilla and alga Stichococcus bacillaris exposed to Pb, Cd and Hg (Gupta et al 1999;Pawlik-Skowron´ska 2002) and in higher plants of Zea mays and Hordeum vulgare (Carrasco-Gil et al 2011). Interestingly, the burst of PC biosynthesis induced by Pb is also here accompanied by a transient depletion of cell GSH pools that may be perceived by plants as indicating an additional need for sulphur (Nocito et al 2007;).…”

Section: Discussionsupporting

confidence: 63%

EDTA-enhanced lead phytoremediation in sunflower (Helianthus annuus L.) hydroponic culture

et al. 2011

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The aim of the present study was to investigate the capability of Sunflower (Helianthus annuus L.) to tolerate and accumulate high amount of lead (Pb) and propose it for soil phytoremediation. To this regard, plants were grown in hydroponics and treated with different Pb concentrations (10 to 160 μM) and a fixed concentration (500 μM) EDTA (ethylene diamine tetra acetic acid) for 14 and 28 days (d). Effects on total biomass production, photosynthetic pigments and protein contents as well as the quantities of non protein thiols (NP-SH), glutathione (GSH), phytochelatins (PCs) and activity of glutathione reductase (GR) were estimated. Results revealed that roots (575 μg g −1 DW) and shoots (135 μg g −1 DW) accumulated Pb after 28 d of exposure, however, addition of EDTA enhanced the Pb accumulation in roots (645 μg g −1 DW) and shoots (255 μg g −1 DW ). Exposure of Pb (28 d) registered a significant (P<0.05) reduction in growth parameters and induction of phytochelatins (P<0.05; r=0.26) plus some of the important antioxidants (P<0.05; r= 0.42), which were positively correlated to metal accumulation. Sunflower exposed at 40 μM of Pb for 28 d synthesized higher quantity of PC 2 (18.5 fold) and PC 3 (10.5 fold), as compared to control. However, the results showed that addition of EDTA resulted in low toxicity compared to Pb alone. These data support the capability of H. annuus L. to accumulate and tolerate significant quantity of Pb and its utility for phytoremediation. This is because of the plant has the capacity to combat metal induced oxidative stress via significant synthesis of NP-SH, GSH and high activity of GR, as it would provide sufficient GSH not only for PCs synthesis but also for antioxidant function.

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Section: Discussionsupporting

confidence: 63%

EDTA-enhanced lead phytoremediation in sunflower (Helianthus annuus L.) hydroponic culture

et al. 2011

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“…This compared to 58% of the arsenic being bound to PCs, as determined by HPLC-ICP-MS. At least for As(III)-PC complexes, it has been demonstrated that the speciation determined in the extract is representative of that found in the plant. Similar measurements have been performed on plants exposed to cadmium [54] and mercury, showing the existence of Cd-PC and Hg-PC complexes in vivo [55].…”

Section: Hplc-esi-ms/msmentioning

confidence: 70%

Quantification of phytochelatins and their metal(loid) complexes: critical assessment of current analytical methodology

Wood

Feldmann

2012

Anal Bioanal Chem

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Whilst there are a variety of methods available for the quantification of biothiols in sample extracts, each has their own inherent advantages and limitations. The ease with which thiols readily oxidise not only hinders their quantification but also alters the speciation profile. The challenge faced by the analyst is not only to preserve the speciation of the sample, but also to select a method which allows the retrieval of the desired information. Given that sulfur is not a chromophore and that it cannot easily be monitored by ICP-MS, a number of direct and indirect methods have been developed for this purpose. In order to assess these methods, they are compared in the context of the measurement of arsenic-phytochelatin complexes in plant extracts. The inherent instability of such complexes, along with the instabilities of reduced glutathione and phytochelatin species,necessitates a rapid and sensitive analytical protocol. Whilst being a specific example, the points raised and discussed in this review will also be applicable to the quantification of biothiols and thiol-metal(loid) species in a wide range of systems other than just the analysis of arsenic-phytochelatin species in plant extracts.

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“…After the clover was cultured in medium containing mercuric chloride and the mercury in roots were analyzed using differential centrifugation, Carrasco-Gil et al found that much mercury in clover roots is located in cell wall [24]. This result was gotten using homogenate and differential centrifugation.…”

Section: Discussionmentioning

confidence: 97%

Mercury Distribution in Tobacco (<i>Nicotiana tabacum</i>) Cell

Chang¹,

Wu²,

Sun³

et al. 2018

ABB

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Problem: Removing mercury from polluted soil using transgenic plants is an ideal method. However, where mercury was stored in plant cell is not clear until now. Methods: Differential centrifugation and laser scanning confocal microscopy were used in this study. Results and findings: Results showed that after mercury was absorbed by tobacco plants, most of the mercury accumulated in roots. Mercury content in root was significantly higher than that in shoot. In the seedlings cultured in MS liquid medium containing 5 µmol/L mercuric chloride, the mercury content in cell wall was 6.6 µg/g. The mercury content in cell membrane fraction was 2.1 µg/g. The mercury content in supernatant fraction was 35.1 µg/g. Most of the mercury accumulated in roots located in liquid fraction, about 15% of the mercury was attached by cell wall. Only a small part assembled in cell membrane. Most of the mercury in liquid fraction located in vacuole. This suggested that after the mercury was accumulated in plant root, most of the mercury was transferred into vacuole. There was no important cellular organ in vacuole. The toxicity of mercury in vacuole will be much lower than that in cell membrane or cellular organs. Recommendation: These results suggested that much work should be focused on how to transfer the mercury in vacuole into the above-ground tissues of the plants in the future.

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Complexation of Hg with phytochelatins is important for plant Hg tolerance

Cited by 110 publications

References 58 publications

EDTA-enhanced lead phytoremediation in sunflower (Helianthus annuus L.) hydroponic culture

EDTA-enhanced lead phytoremediation in sunflower (Helianthus annuus L.) hydroponic culture

Quantification of phytochelatins and their metal(loid) complexes: critical assessment of current analytical methodology

Mercury Distribution in Tobacco (<i>Nicotiana tabacum</i>) Cell

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Complexation of Hg with phytochelatins is important for plant Hg tolerance

Cited by 110 publications

References 58 publications

EDTA-enhanced lead phytoremediation in sunflower (Helianthus annuus L.) hydroponic culture

EDTA-enhanced lead phytoremediation in sunflower (Helianthus annuus L.) hydroponic culture

Quantification of phytochelatins and their metal(loid) complexes: critical assessment of current analytical methodology

Mercury Distribution in Tobacco (&lt;i&gt;Nicotiana tabacum&lt;/i&gt;) Cell

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Mercury Distribution in Tobacco (<i>Nicotiana tabacum</i>) Cell