Complexation of zinc by exudates from Skeletonema costatum grown in culture

Imber, B.E; Robinson, M. G.; Ortega, A.M; Burton, J. D.

doi:10.1016/0304-4203(85)90018-0

Cited by 21 publications

(12 citation statements)

References 18 publications

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“…The production of ligands with affinity for copper has been demonstrated for laboratory cultures of algae (e.g. McKnight and Morel 1978), and natural assemblages of phytoplankton have been observed to produce ligands with affinity for zinc (Imber et al 1985) and specific for iron (Trick et al 1983).…”

Section: Discussionmentioning

confidence: 99%

Copper complexation in the Northeast Pacific

Coale

Bruland

1988

Limnology & Oceanography

479

256

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Copper titrations were conducted at sea with differential pulse anodic stripping voltammetry to examine the degree to which copper was associated with organic ligands. Greater than 99.7% of the total dissolved copper in surface waters of the central Northeast Pacific shallower than 200 m was estimated to be associated with strong organic complexes. Below 200 m, increasing proportions of inorganic or labile copper spccics were observed. At middepths (1,000 m), about 50-70% of the total dissolved copper was in the organically complexed form. Whereas total copper varies by a factor of only three from the surface to middepths (0.6-I .8 nM), copper complexation gives rise to extremely low cupric ion activities in surface waters ({Cu!'} = 1.4 x lo-l4 M) and higher values at middepth ({Cuz-I} = lo-I1 M)-a variation of three orders of magnitude. Two classes of copperbinding ligands were found to be responsible for this complexation: an extremely strong ligand class [log K'cond (cu,j = 11.5 J at low concentrations (-1.8 nM) -which dominated copper complexation in the surface waters and decreased with depth, and a weaker class of ligands [log K'cond(Cu3 = 8.51 at higher concentrations (8-10 nM) which was observed throughout the water column and showed no apparent structure in its vertical distribution. These findings have significant implications concerning the toxicity and bioavailability of copper in open ocean systems.

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Section: Discussionmentioning

confidence: 99%

Copper complexation in the Northeast Pacific

Coale

Bruland

1988

Limnology & Oceanography

479

256

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“…However, there is evidence for biological production of ligands by phytoplankton. Cultures of the diatoms Skeletonema costaturn and Thalassiosira weissjogii and the coccolithophore Emiliania huxleyi produced organic exudates with Zn binding capability (Imber and Robinson, 1983;Imber et al, 1985;Vasconcelos et al, 2002). Muller et al (2003) observed an increase in Zn binding ligands in a mesocosm experiment that was coincident with an increase in dead E. huxleyi cells, which suggests ligand release may be passive.…”

Section: Introductionmentioning

confidence: 99%

Relating the biogeochemistries of zinc, cobalt, and phosphorus to phytoplankton activities in the sea

Wisniewski¹

2006

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This thesis explores the potential of zinc, cobalt, and phosphorus to influence primary production in the subarctic North Pacific, the Bering Sea, and the North Atlantic Ocean, In the North Pacific and Bering Sea, total zinc concentrations were measured along a near-surface transect and in selected deep profiles. Zinc speciation was also measured with a novel anodic stripping voltammetry method, and the results were consistent with previous studies using different methods. The potential for zinc to impact primary production in the North Pacific was demonstrated in a shipboard incubation and by comparing two phytoplankton pigment markers to total zinc and free zinc ion concentrations. In the North Atlantic, total dissolved zinc and cobalt concentrations were measured and compared to concentrations of dissolved inorganic phosphorus and chlorophyll. In some areas of the North Atlantic the concentrations of zinc and cobalt were decoupled. The relationship between cobalt and inorganic phosphorus suggests that cobalt drawdown may be related to a high alkaline phosphatase related demand at low phosphorus concentrations. This trend compliments a shipboard incubation where alkaline phosphatase activities increased after cobalt addition. The presence of measurable alkaline phosphatase activity indicated that the phytoplankton community in the Sargasso Sea was experiencing phosphorus stress. Shipboard incubations generally confirmed this with inorganic phosphorus additions resulting in chlorophyll increases at 4 out of 5 stations. Further, the addition of dissolved organic phosphorus, as either a phosphate monoester or a phosphonate compound, resulted in a chlorophyll increase in 3 out of 3 incubations. This suggests that dissolved organic phosphorus may be an important phosphorus source for phytoplankton in low phosphorus environments and that the ability to use phosphonates may be more widespread than previously recognized. Overall, this thesis adds to our understanding of how the nutrients phosphorus, zinc, and cobalt may influence primary production.

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“…4), with larger changes in both [L] and salinity. It is obvious that the input of terrestrial humic materials from river Comparison with the culture experiment Many workers have observed the release of trace metal-complexing DOM by cultured phytoplankton, especially in the stationary or post-stationary phase of a batch culture (Swallow et al 1978, McKnight & More1 1979, Fisher & Fabris 1982, Imber et al 1985, Zhou & Wangersky 1985, Seritti et al 1986). With a cage culture turbidostat system and the modified Sep-Pak C18 column extraction method, we have found a significant production of copper-complexing organic ligands by the marine diatom Phaeodactylum tn'cornuturn in log phase growth (Zhou & Wangersky 1989b).…”

Section: Resultsmentioning

confidence: 99%

“…Many laboratory culture experiments have demonstrated that marine phytoplankton can produce significant amounts of organic ligands, during both the active growth phase in a cage culture turbidostat (Zhou & Wangersky 1985, 1989b, and in stationary phase in batch cultures (Swallow et al 1978, McKnight & More1 1979, Fisher & Fabris 1982, Imber et al 1985, Zhou & Wangersky 1985, Seritti 1986). The extracellular production of dissolved organic matter (DOM) by phytoplankton could thus be an important source of organic ligands in seawater.…”

Section: Introductionmentioning

confidence: 99%

Changes in copper-complexing organic ligands during spring blooms in the coastal waters of Nova Scotia, Canada

Zhou¹,

Wangersky²

1989

Mar. Ecol. Prog. Ser.

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Copper-complexing organic ligands were produced by actively-growing phytoplankton during spring blooms in the Bedford Basin and the Northwest Arm, Halifax. Nova Scotia. Iatroscan TLC/ FID and TLC/AAS analysis showed the naturally occurring ligands to be qualitatively similar to those produced by phytoplankton grown in batch and continuous culture. The Bedford Basin bloom was interrupted by an extremely heavy rainfall, resulting in a large contribution of material, including organic ligands, from terrestrial sources. The ligand contributed from this source could be shown to be qualitatively different from that produced in situ by bloom organisms.

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Complexation of zinc by exudates from Skeletonema costatum grown in culture

Cited by 21 publications

References 18 publications

Copper complexation in the Northeast Pacific

Copper complexation in the Northeast Pacific

Relating the biogeochemistries of zinc, cobalt, and phosphorus to phytoplankton activities in the sea

Changes in copper-complexing organic ligands during spring blooms in the coastal waters of Nova Scotia, Canada

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