Hydrogen peroxide (H2O2) is a key intermediate in many biological and environmental processes, and there is a range of analytical methods available for its quantification, e.g. photometric analysis, 1,2 fluorometry, 3,4 chemiluminescence 5,6 and electroanalytical techniques. 7,8 General difficulties encountered in the analysis of such transient and reactive species include the choice of a reliable standardization method, the stability and purity of reagents and low-level interferences. 9 The application of the fluorescent dimerization of p-hydroxyphenylacetic acid (P-HPAA) to environmental matrices offers some advantages over alternate methods and provides an analytically consistent method for a wide range of atmospheric and oceanic measurements of H2O2. 10 The fluorescent P-HPAA dimer is a stable product, which can be quantified by fluorescence several days after preparation, eliminating difficulties caused by the decomposition of peroxides in stored samples.11 Also, many of the interferences that plagued the early chemiluminescent methods do not occur with P-HPAA. 11,12 However, the P-HPAA method often employs two biological enzymes, horseradish peroxidase and catalase, which are both expensive and unstable. 13 It is inconvenient, especially for field measurements, to prepare daily the analytical reagent in order to prevent any possible loss of the enzyme activity over time.