Complexes of Ericchrome Black T with Calcium and Magnesium

Young, Allen; Sweet, Thomas R.

doi:10.1021/ac60099a026

Cited by 45 publications

(16 citation statements)

References 7 publications

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“…EBT is blue between pH 7 and 11 (Bell, 1977). The formation of the magnesium complex of EBT is accompanied by a spectral change (Young and Sweet, 1955). We used this to determine its dissociation constant for Mg 2ϩ under our experimental conditions by measuring the absorbance as a function of [Mg 2ϩ ] and fitting to the following equation: …”

Section: Measurement Of Mitochondrial Respiration and Intactness-intactmentioning

confidence: 99%

The Role of Pyridine Dinucleotides in Regulating the Permeability of the Mitochondrial Outer Membrane

Lee¹,

Colombini

1996

Journal of Biological Chemistry

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Both NADH and NADPH reduce the permeability of the mitochondrial outer membrane to ADP. This is specific for the outer membrane and uncorrelated with the respiratory control ratio. This could result in a 7-fold difference between the concentration of ADP in the intermembrane space and that in the external environment (at 5 M ADP). In both cases the permeability declines by a factor of 5, but NADH is more potent: K D ‫؍‬ 86 M for NADH versus 580 M for NADPH. The lower apparent affinity for NADPH is partly explained by Mg 2؉ -NADPH being the active species, and under our conditions only 30% of the NADPH is in this form. The corrected K D is 184 M. Free NADH has the same charge as the Mg 2؉ -NADPH complex, and thus both likely bind to the same site. The ability of NADH and NADPH to induce the closure of reconstituted VDAC channels is consistent with VDAC being the main pathway for metabolite flow across the outer membrane. Oncotic pressure, effective at inducing VDAC closure, also decreases the outer membrane permeability. Thus, in the presence of cytosolic colloidal osmotic pressure NAD(P)H may inhibit mitochondrial catabolic pathways and divert reducing equivalents to anabolic pathways.

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Section: Measurement Of Mitochondrial Respiration and Intactness-intactmentioning

confidence: 99%

The Role of Pyridine Dinucleotides in Regulating the Permeability of the Mitochondrial Outer Membrane

Lee¹,

Colombini

1996

Journal of Biological Chemistry

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“…Similarly the spectra of the species CaD"*, MgD" and HD = are given in Figure 7. These spectra are the same as given by On the other hand, one of the American groups (27) found the absorbancy of a I.299 x 10**-5 M solution of the dye at pH 9.32 to be 0.78 using 5 mm. cells; using these data a molar extinction coefficient of 12,000 was computed.…”

Section: Summary Of Analytical Resultssupporting

confidence: 67%

Hydroxyazo dyes as metal ion indicators

Lindstrom

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Introduction Discovery and early investigation Use of Eriochrome Black T as a metal ion indicator 3 Preparation of a Pure Form of Eriochrome Black T. . Attempts to purify commercial samples of the dye Preliminary study of the magnesium dye compound at pH 8 by the log-ratio method 18 Synthesis of Eriochrome Slack T 23 Procedure for the preparation of a pure form of Eriochrome Black T 28 Conclusions 31 Analysis and Characterization of Eriochrome Black T Crystals Elemental analysis Detection and determination of dime t lay lamine from the crystalline dye 33 Determination of neutralization equivalent. . 35 Determination of moisture content 39 Summary of analytical results 40 Conclusions Free Acid Form of Eriochrome Black T Preparation of the free sulfonic acid Study of the free dye acid Eriochrome Black T and Its Calcium and Magnesium Derivatives Apparatus and materials Acid dissociation constants of Eriochrome Black T ..«.a 45 Beer's law study of the purified dye and the molar extinction coefficient 55 Log-ratio study of the calcium and magnesium. compounds of Eriochrome Black T

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“…Similarly the spectra of the species CaD"*, MgD" and HD = are given in Figure 7. These spectra are the same as given by (27) found the absorbancy of a I.299 x 10**-5 M solution of the dye at pH 9.32 to be 0.78 using 5 mm. cells; using these data a molar extinction coefficient of 12,000 was computed.…”

Section: Eriochrome Black T and Its Calcium And Magnesium Derivativessupporting

confidence: 53%

“…The molar extinction coefficient of Eriochrome Black T at pH 10 and 615 mji, the absorbancy maximum, was measured and found to be 32,300 compared to 29,600, the value re ported by Schwarzenbach ( The properties of the dye as a complex!ng agent were found to be quite different from the properties given by the American workers (12,27) in their attack on the work of Schwarzenbach.…”

Section: Continuous Variations Study Of the Magnesium Compound Of Erimentioning

Complexes of Ericchrome Black T with Calcium and Magnesium

Cited by 45 publications

References 7 publications

The Role of Pyridine Dinucleotides in Regulating the Permeability of the Mitochondrial Outer Membrane

The Role of Pyridine Dinucleotides in Regulating the Permeability of the Mitochondrial Outer Membrane

Hydroxyazo dyes as metal ion indicators

Hydroxyazo dyes as metal ion indicators

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