Complexing and photoprocesses in a PRODAN molecule

Artyukhov, V. Ya.; Zharkova, O. M.; Morozova, Yu. P.

doi:10.1007/s11182-005-0049-6

Cited by 2 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observation of two emission bands is consistent with the presence of two distinct bands reported in several theoretical and experimental investigations of PRODAN absorption transitions. 18,19,21,67,68 Definitive assignments of these bands have not been reported, but the variation in emission wavelength from both states with solvent polarity indicates that both have substantial charge transfer character. It is noteworthy that the redder spectrum decays at the slower rate and is more intense than the blue component in toluene and acetonitrile.…”

Section: Resultsmentioning

confidence: 98%

Spectral Heterogeneity of PRODAN Fluorescence in Isotropic Solvents Revealed by Multivariate Photokinetic Analysis

et al. 2008

View full text Add to dashboard Cite

This paper describes a multivariate analysis of the fluorescence emission of 6-propionyl-2-dimethylaminonaphthalene (PRODAN) in a series of isotropic solvents of differing polarity and hydrogen-bonding ability. Multivariate methods distill the essential features from spectral data matrices so that the structural details that are embedded within the data are revealed to the analyst. In the aprotic solvents investigated, the analysis reveals a pair of emission components that have emission maxima that scale with the orientational polarizability. In the alcohols, short-lived, polarity-independent blue bands tentatively attributed to neutral hydrogen-bonded solute-solvent complexes form and relax prior to emission from paired bands that have Stokes shifts that scale with the solvent hydrogen-bonding ability rather than the polarity. In water, the short-lived blue bands were not observed, but the shift in the paired bands did scale with the solvent hydrogen-bonding ability.

show abstract

Section: Resultsmentioning

confidence: 98%

Spectral Heterogeneity of PRODAN Fluorescence in Isotropic Solvents Revealed by Multivariate Photokinetic Analysis

et al. 2008

View full text Add to dashboard Cite

show abstract

“…PRODAN and LAURDAN are known to be used in several membrane studies. Their photophysical properties have been extensively studied and the existence of several states has been established, of PRODAN and its analogs, in both the ground and excited states [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. It was shown that the fluorescence characteristics of PRODAN in 1,4-dioxane are due to two forms of the dye in the ground and two forms of PRODAN in the excited state, which differ in the magnitude of the dipole moment and the conformation of the carbonyl group [19].…”

Section: Introductionmentioning

confidence: 99%

Photophysical Properties of BADAN Revealed in the Study of GGBP Structural Transitions

Fonin

Silonov

Antifeeva

et al. 2021

IJMS

View full text Add to dashboard Cite

The fluorescent dye BADAN (6-bromoacetyl-2-dimetylaminonaphtalene) is widely used in various fields of life sciences, however, the photophysical properties of BADAN are not fully understood. The study of the spectral properties of BADAN attached to a number of mutant forms of GGBP, as well as changes in its spectral characteristics during structural changes in proteins, allowed to shed light on the photophysical properties of BADAN. It was shown that spectral properties of BADAN are determined by at least one non-fluorescent and two fluorescent isomers with overlapping absorbing bands. It was found that BADAN fluorescence is determined by the unsolvated “PICT” (planar intramolecular charge transfer state) and solvated “TICT” (twisted intramolecular charge transfer state) excited states. While “TICT” state can be formed both as a result of the “PICT” state solvation and as a result of light absorption by the solvated ground state of the dye. BADAN fluorescence linked to GGBP/H152C apoform is quenched by Trp 183, but this effect is inhibited by glucose intercalation. New details of the changes in the spectral characteristics of BADAN during the unfolding of the protein apo and holoforms have been obtained.

show abstract

Complexing and photoprocesses in a PRODAN molecule

Cited by 2 publications

References 5 publications

Spectral Heterogeneity of PRODAN Fluorescence in Isotropic Solvents Revealed by Multivariate Photokinetic Analysis

Spectral Heterogeneity of PRODAN Fluorescence in Isotropic Solvents Revealed by Multivariate Photokinetic Analysis

Photophysical Properties of BADAN Revealed in the Study of GGBP Structural Transitions

Contact Info

Product

Resources

About