Complexity of the State of Immunological Tolerance

Hasek, M; Chutná, J

doi:10.1111/j.1600-065x.1979.tb00282.x

Cited by 38 publications

(8 citation statements)

References 66 publications

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“…H-2K expression also appears to be necessary for both induction of CTL and susceptibility to lysis in the response to murine leukaemia virus antigens on syngeneic AKR leukaemias (14). It has been suggested that H-2K antigen may be more immunogenic than H-2D, not only as an allo-antigen (15) but also as a syngeneic Yestriction element' in the response to other antigens (16; 17), whereas H-2D may be more tolerogenic (18). Eisenbach et al (7) hypothesized that such a differential role lor K^ and D'' also applies in syngeneic and allogeneic responses to 3LL.…”

Section: Discussionmentioning

confidence: 99%

In vivo H‐2K and H‐2D antigen expression In two allogeneic mouse tumours of low immunogenicity

Ashley

Kotlarski

1987

Immunol Cell Biol

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Summary The B16 melanoma of C57BL/6 mice immunizes very poorly, even against its own major histocompatibility complex (MHC) antigens. B16 cells expressed both H‐2K and H‐2D antigens in vitro as judged by binding of monoclonal antibodies to these antigens in indirect immunofluorescence staining. The in vivo MHC antigen expression of B16 was examined and compared with that of a second C57BL/6 tumour, the Lewis lung carcinoma (3LL). whose defective immunogenicity has been attributed to a selective deficiency‐ in H‐2K antigen expression. We found that 125I‐labeiled cells of both tumours expressed sufficient allo‐antigen in vivo to be lysed in BALB/c mice which had been pre‐immunized with C57BL/6 lymphoid cells. 125I‐B16 cells were also lysed in MHC‐recombinant mice which had been immunized against either H‐2Kb or H‐2Db, indicating that B16 cells express both of these MHC antigens in vivo. This contrasted with our findings with 125I‐3LL cells which were destroyed in mice immunized against H‐2Db but not in those immunized against H‐2Kb. Thus, B16 illustrates a different deficiency in tumour cell immunogenicity which appears not to be attributable to an absence of either of the class 1 MHC antigens.

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Section: Discussionmentioning

confidence: 99%

In vivo H‐2K and H‐2D antigen expression In two allogeneic mouse tumours of low immunogenicity

Ashley

Kotlarski

1987

Immunol Cell Biol

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“…Spleen cells from both adult mice given TLI (11) and newborn mice (12,13) contain nonspecific suppressor cells of the mixed leukocyte reaction (MLR) before marrow transplantation. Recent evidence suggests that after transplantation of allogeneic or semiallogeneic lymphoid cells, both TLI-treated adult mice (14,15) and newborn mice (16) or rats (17)(18)(19)(20)(21)(22) develop suppressor cells of transplantation immunity which are specific for the tolerizing tissue antigens.…”

mentioning

confidence: 99%

Spleen cells from adult mice given total lymphoid irradiation or from newborn mice have similar regulatory effects in the mixed leukocyte reaction. I. Generation of antigen-specific suppressor cells in the mixed leukocyte reaction after the addition of spleen cells from adult mice given total lymphoid irradiation.

Okada¹,

Strober²

1982

The Journal of Experimental Medicine

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We added spleen cells from adult BALB/c mice treated with total lymphoid irradiation (TLI) to the mixed leukocyte reaction (MLR) using a variety of responder and stimulator cells. The spleen cells nonspecifically suppressed the uptake of [3H]-thymidine and the generation of cytolytic cells regardless of the responder-stimulator combination used. We also examined the effect of the spleen cells on the generation of antigen-nonspecific and antigen-specific suppressor cells in the MLR. The experimental results suggest that the spleen cells from TLI-treated mice inhibit the generation of nonspecific suppressor cells, but do not inhibit the generation of antigen-specific suppressor cells. Thus, alloantigenic stimulation of normal responder cells in vitro in the presence of spleen cells from TLI-treated mice generates large numbers of antigen-specific suppressor cells, but few cytolytic cells or nonspecific suppressor cells. Similar nonspecific inhibition of the MLR was observed with neonatal spleen cells. This in vitro system provides a regulatory model for the induction and maintenance of tolerance in vivo, in which adult mice given TLI or neonatal mice accept allogeneic bone marrow transplants without graft-vs.-host disease.

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“…However, studies in fully allogeneic r.at chimeras that survive transient GVHD suggest that suppressor cells maintain the tolerant state (11). Multiple mechanisms may be operative in these instances of graft vs. host tolerance as seems to be the case for neonatal tolerance (12). Our experiments in progress attempt to determine whether graft vs. host tolerance in TLI chimeras is mediated by antigen-specific suppressor T cells of donor origin or by deletion of specific host-reactive lymphocyte clones.…”

Section: Development Of Chimerism In Irradiated Balb/c Recipients Givmentioning

confidence: 95%

Cellular basis of graft versus host tolerance in chimeras prepared with total lymphoid irradiation.

Gottlieb¹,

Strober²,

Kaplan³

1980

The Journal of Experimental Medicine

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BALB/c mice given allogeneic (C57BL/Ka) bone marrow cells after toal lymphoid irradiation become stable chimeras approximately 80% donor-type and 20% host-type cells in the spleen. The chimeras doe not develop graft vs. host disease (GVHD). Purified cells of C57BL/Ka origin from the chimeras mediated GVHD in lightly irradiated C3H (third party), but not in BALB/c (host-strain) mice. Thus graft vs. host tolerance in the chimeras could not be explained by complete immunodeficiency of donor-type cells, serum blocking factors, or suppressor cells of host (BALB/c) origin. Clonal deletion or suppression of lymphocytes reactive with host tissues remain possible explanations. The transfer of donor-type chimeric spleen cells to BALB/c recipients given 500-550 rad whole-body irradiation WBI led to stable mixed chimerism in approximately 50% of recipients. The cells were presumably acting as tolerogens because similarly irradiated BALB/c mice given (BALB/c X C57BL/Ka)F1 spleen or bone marrow cells also became stable mixed chimeras.

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Complexity of the State of Immunological Tolerance

Cited by 38 publications

References 66 publications

In vivo H‐2K and H‐2D antigen expression In two allogeneic mouse tumours of low immunogenicity

In vivo H‐2K and H‐2D antigen expression In two allogeneic mouse tumours of low immunogenicity

Cellular basis of graft versus host tolerance in chimeras prepared with total lymphoid irradiation.

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