In this study, the sedimentation states of particles in high-concentration slurries were elucidated by monitoring their internal states. We prepared transparent high-concentration silica slurries by adjusting the refractive index of the aqueous glycerol liquid in which the particles were dispersed to match that of the silica particles. In addition, a fluorescent dye was dissolved in the liquid. Then, we directly observed the individual and flocculated particles in the slurries during sedimentation by confocal laser scanning fluorescent microscopy. The particles were found to sediment very slowly while exhibiting fluctuating motion. The particle sedimentation rate in the high-concentration slurry with the aqueous glycerol solution (η=0.068Pa·s) and a particle volume fraction on the order of 0.3 was determined to be 1.58 ± 0.66 μm·min−1 on the basis of the obtained image sequences for 24.9 h. In-situ observation provides a large amount of information about the sedimentation behavior of particles in condensed matter.