Complicated Meningitis caused by a rare serotype of Haemophilus influenzae in Portugal

Calado, Rita; Betencourt, Célia; Gonçalves, Helder; Cristino, N.; Calhau, Paulo; Bajanca-Lavado, Paula

doi:10.1016/j.diagmicrobio.2010.08.029

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…influenzae serotype d was isolated from both the cerebrospinal fluid and the blood of adults with meningitis. 60 Moreover, it was an etiological agent of meningitis in infant, 4 maternal and neonatal sepsis, 61 mild respiratory disease in neonates, 53 of pneumonia, empyema, and shock. 6 According to Sakano et al, 62 this type of bacteria may be an important causative agent in immunodeficient patients.…”

Section: Discussionmentioning

confidence: 99%

“…The encapsulate strains of H. influenzae (mainly serotype b—Hib, but also serotypes a, c—f) are important in the pathogenesis of invasive infections and chronic or recurrent diseases that are most often reported in children and rarely in adults. 4 – 6 For a long time Hib was considered as a major pathogen of young children. Since the introduction of Hib vaccines, invasive H. influenzae disease has been significantly eliminated.…”

Section: Introductionmentioning

confidence: 99%

“… 7 – 9 The increasing number of reports about other than Hib serotypes (mainly a, e, and f serotypes), which may be responsible for diseases, are spreading globally. 4 , 10 – 13 Today it is known that H. influenzae without capsular polysaccharides, referred to as nontypeable (nonencapsulated) H. influenzae (NTHi), have also been associated with chronic or invasive diseases. 14 – 16 NTHi remain important as causes of respiratory infections and are frequently associated with otitis media, chronic bronchitis, and community-acquired pneumonia.…”

Section: Introductionmentioning

confidence: 99%

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Nasopharyngeal and Adenoid Colonization by Haemophilus influenzae and Haemophilus parainfluenzae in Children Undergoing Adenoidectomy and the Ability of Bacterial Isolates to Biofilm Production

et al. 2015

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Haemophili are pathogenic or opportunistic bacteria often colonizing the upper respiratory tract mucosa. The prevalence of Haemophilus influenzae (with serotypes distribution), and H. parainfluenzae in the nasopharynx and/or the adenoid core in children with recurrent pharyngotonsillitis undergoing adenoidectomy was assessed. Haemophili isolates were investigated for their ability to biofilm production.Nasopharyngeal swabs and the adenoid core were collected from 164 children who underwent adenoidectomy (2–5 years old). Bacteria were identified by the standard methods. Serotyping of H. influenzae was performed using polyclonal and monoclonal antisera. Biofilm formation was detected spectrophotometrically using 96-well microplates and 0.1% crystal violet.Ninety seven percent (159/164) children who underwent adenoidectomy were colonized by Haemophilus spp. The adenoid core was colonized in 99.4% (158/159) children, whereas the nasopharynx in 47.2% (75/159) children (P < 0.0001). In 32% (51/159) children only encapsulated (typeable) isolates of H. influenzae were identified, in 22.6% (36/159) children only (nonencapsulated) H. influenzae NTHi (nonencapsulated) isolates were present, whereas 7.5% (12/159) children were colonized by both types. 14.5% (23/159) children were colonized by untypeable (rough) H. influenzae. In 22% (35/159) children H. influenzae serotype d was isolated. Totally, 192 isolates of H. influenzae, 96 isolates of H. parainfluenzae and 14 isolates of other Haemophilus spp. were selected. In 20.1% (32/159) children 2 or 3 phenotypically different isolates of the same species (H. influenzae or H. parainfluenzae) or serotypes (H. influenzae) were identified in 1 child. 67.2% (129/192) isolates of H. influenzae, 56.3% (54/96) isolates of H. parainfluenzae and 85.7% (12/14) isolates of other Haemophilus spp. were positive for biofilm production. Statistically significant differences (P = 0.0029) among H. parainfluenzae biofilmproducers and nonproducers in the adenoid core and the nasopharynx were detected.H. influenzae and H. parainfluenzae carriage rate was comparatively higher in the adenoid core than that in the nasopharynx in children undergoing adenoidectomy, suggesting that their involvement in chronic adenoiditis. The growth in the biofilm seems to be an important feature of haemophili colonizing the upper respiratory tract responsible for their persistence.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nasopharyngeal and Adenoid Colonization by Haemophilus influenzae and Haemophilus parainfluenzae in Children Undergoing Adenoidectomy and the Ability of Bacterial Isolates to Biofilm Production

et al. 2015

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Characteristics of Haemophilus influenzae invasive isolates from Portugal following routine childhood vaccination against H. influenzae serotype b (2002–2010)

Bajanca-Lavado

Simões

Betencourt

et al. 2013

Eur J Clin Microbiol Infect Dis

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We aimed to characterize Haemophilus influenzae invasive isolates recovered in Portugal over a 9-year period (2002-2010) following the inclusion of H. influenzae serotype b (Hib) conjugate vaccination in the National Immunization Program (NIP) in the year 2000 and compare the results with those obtained in a similar study from the pre-vaccination era (1989-2001) previously described by us. As part of a laboratory-based passive surveillance system, 144 invasive isolates obtained in 28 Portuguese hospitals were received at the National Reference Laboratory for Bacterial Respiratory Infections and were characterized. Capsular types and antibiotic susceptibility patterns were determined. The ftsI gene encoding PBP3 was sequenced for β-lactamase-negative ampicillin-resistant (BLNAR) isolates. Genetic relatedness among isolates was examined by multilocus sequencing typing (MLST). Most isolates (77.1%) were non-capsulated, a significant increase compared to the pre-vaccination era (19.0%, p < 0.001). Serotype b strains decreased significantly (from 81.0 to 13.2%, p < 0.001) and serotype f increased significantly (from 0.8 to 6.9%, p = 0.03). Ten percent of the isolates were β-lactamase producers, a value lower than that previously observed (26.9%, p = 0.005). Eight percent of all isolates were BLNAR. A high genetic diversity among non-capsulated isolates was found. By contrast, capsulated isolates were clonal. The implementation of Hib vaccination has resulted in a significant decline in the proportion of serotype b H. influenzae invasive disease isolates. Most episodes of invasive disease occurring in Portugal are now due to fully susceptible, highly diverse, non-capsulated strains. Given the evolving dynamics of this pathogen and the increase in non-type b capsulated isolates, continuous surveillance is needed.

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Rare serotype c Haemophilus influenzae invasive isolate: characterization of the first case in Portugal

Bajanca-Lavado,

Pinto,

Carvalho

et al. 2024

Eur J Clin Microbiol Infect Dis

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Complicated Meningitis caused by a rare serotype of Haemophilus influenzae in Portugal

Cited by 6 publications

References 17 publications

Nasopharyngeal and Adenoid Colonization by Haemophilus influenzae and Haemophilus parainfluenzae in Children Undergoing Adenoidectomy and the Ability of Bacterial Isolates to Biofilm Production

Nasopharyngeal and Adenoid Colonization by Haemophilus influenzae and Haemophilus parainfluenzae in Children Undergoing Adenoidectomy and the Ability of Bacterial Isolates to Biofilm Production

Characteristics of Haemophilus influenzae invasive isolates from Portugal following routine childhood vaccination against H. influenzae serotype b (2002–2010)

Rare serotype c Haemophilus influenzae invasive isolate: characterization of the first case in Portugal

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