Complications dawn for kinetochore regulation by Aurora

Nannas, Natalie J.; Murray, Andrew W.

doi:10.1073/pnas.1214115109

Cited by 5 publications

(6 citation statements)

References 17 publications

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“…The AurB kinase and INCENP are two subunits of the four-subunit CPC complex, which phosphorylates downstream targets to regulate multiple processes of mitosis and cytokinesis [75, 76]. Spc25 is a subunit of the Ndc80 outer kinetochore complex, which is phosphorylated by the CPC to regulate microtubule-kinetochore attachments [77, 78]. The Tum protein is a Rac-GAP protein that is phosphorylated by the CPC and regulates the kinesin Pav for proper cytokinesis [79].…”

Section: Resultsmentioning

confidence: 99%

A Cyclin A—Myb-MuvB—Aurora B network regulates the choice between mitotic cycles and polyploid endoreplication cycles

et al. 2019

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Endoreplication is a cell cycle variant that entails cell growth and periodic genome duplication without cell division, and results in large, polyploid cells. Cells switch from mitotic cycles to endoreplication cycles during development, and also in response to conditional stimuli during wound healing, regeneration, aging, and cancer. In this study, we use integrated approaches in Drosophila to determine how mitotic cycles are remodeled into endoreplication cycles, and how similar this remodeling is between induced and developmental endoreplicating cells (iECs and devECs). Our evidence suggests that Cyclin A / CDK directly activates the Myb-MuvB (MMB) complex to induce transcription of a battery of genes required for mitosis, and that repression of CDK activity dampens this MMB mitotic transcriptome to promote endoreplication in both iECs and devECs. iECs and devECs differed, however, in that devECs had reduced expression of E2F1-dependent genes that function in S phase, whereas repression of the MMB transcriptome in iECs was sufficient to induce endoreplication without a reduction in S phase gene expression. Among the MMB regulated genes, knockdown of AurB protein and other subunits of the chromosomal passenger complex (CPC) induced endoreplication, as did knockdown of CPC-regulated cytokinetic, but not kinetochore, proteins. Together, our results indicate that the status of a CycA—Myb-MuvB—AurB network determines the decision to commit to mitosis or switch to endoreplication in both iECs and devECs, and suggest that regulation of different steps of this network may explain the known diversity of polyploid cycle types in development and disease.

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Section: Resultsmentioning

confidence: 99%

A Cyclin A—Myb-MuvB—Aurora B network regulates the choice between mitotic cycles and polyploid endoreplication cycles

et al. 2019

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“…For instance, it has been documented that endopolyploidy affecting some cells or tissues emerge during development or under stress conditions (Cao et al, 2017; González-Rosa et al, 2018; Losick et al, 2013). In different organisms, several cell cycle regulators were found to be responsible for the switch from normal mitosis to endomitosis/ endoreplication cycles, specifically when they are downregulated or their function is affected (Diril et al, 2012; Nannas and Murray, 2012; Rotelli et al, 2019; Sauer et al, 1995). Among these regulators, Cyclin A/Cyclin dependent kinase 1 and Aurora B kinase play a crucial role (Adams et al, 2001; Diril et al, 2012; Giet and Glover, 2001; Nannas and Murray, 2012; Rotelli et al, 2019; Sauer et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…In different organisms, several cell cycle regulators were found to be responsible for the switch from normal mitosis to endomitosis/ endoreplication cycles, specifically when they are downregulated or their function is affected (Diril et al, 2012; Nannas and Murray, 2012; Rotelli et al, 2019; Sauer et al, 1995). Among these regulators, Cyclin A/Cyclin dependent kinase 1 and Aurora B kinase play a crucial role (Adams et al, 2001; Diril et al, 2012; Giet and Glover, 2001; Nannas and Murray, 2012; Rotelli et al, 2019; Sauer et al, 1995). Moreover, in their elegant work, Rotelli and co-authors (Rotelli et al 2019) showed the link between concentration of CDC1 and AurB to the stringency of effects on the cell cycle.…”

Section: Discussionmentioning

confidence: 99%

Challenges and costs of asexuality: Variation in premeiotic genome duplication in gynogenetic hybrids from Cobitis taenia complex

Dedukh

Marta

Janko

2021

Preprint

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The transition from sexual reproduction to asexuality is often triggered by hybridization. The gametogenesis of many hybrid asexuals involves a stage of premeiotic genomic endoreduplication leading to the production of clonal gametes and bypassing genomic incompatibilities that would normally cause hybrid sterility. However, it is still not clear at what gametogenic stage the endoreplication occurs, how many gonial cells it affects, and whether its rate differs among clonal lineages. Here, we investigated meiotic and premeiotic cells of diploid and triploid hybrids of spined loaches (Cypriniformes: Cobitis) that reproduce by gynogenesis. We found that naturally as well as experimentally produced F1 hybrid strains undergo an obligatory genome duplication event to achieve asexuality, occurring in the gonocytes just before entering meiosis or, rarely, one or few divisions before meiosis. Surprisingly, however, the genome endoreplication was observed only in a minor fraction of the hybrid's gonocytes, while the vast majority were unable to duplicate their genomes and consequently could not proceed beyond pachytene due to defects in pairing and bivalent formation. We also noted that the rate of endoreplication was significantly higher among gonocytes of hybrids from successful natural clones than of experimentally produced F1 hybrids, indicating that interclonal selection may favor lineages that maximize the rate of premeiotic endoreduplication. We conclude that asexuality and hybrid sterility are intimately related phenomena and the transition from sexual reproduction to asexuality must overcome significant problems with genome incompatibilities with possible impact on reproductive potential.

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“…For instance, it has been documented that endopolyploidy affecting some cells or tissues emerge during development or under stress conditions [78][79][80]. In different organisms, several cell cycle regulators were found to be responsible for the switch from normal mitosis to endomitosis/endoreplication cycles, specifically when they are downregulated or their function is affected [81][82][83][84]. Among these regulators, Cyclin A/Cyclin dependent kinase 1 and Aurora B kinase play a crucial role [81][82][83][84][85][86].…”

Section: Initiation Of Premeiotic Genome Duplicationmentioning

confidence: 99%

Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from Cobitis taenia Complex

Dedukh

Marta

Janko

2021

IJMS

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The transition from sexual reproduction to asexuality is often triggered by hybridization. The gametogenesis of many hybrid asexuals involves premeiotic genome endoreplication leading to bypass hybrid sterility and forming clonal gametes. However, it is still not clear when endoreplication occurs, how many gonial cells it affects and whether its rate differs among clonal lineages. Here, we investigated meiotic and premeiotic cells of diploid and triploid hybrids of spined loaches (Cypriniformes: Cobitis) that reproduce by gynogenesis. We found that in naturally and experimentally produced F1 hybrids asexuality is achieved by genome endoreplication, which occurs in gonocytes just before entering meiosis or, rarely, one or a few divisions before meiosis. However, genome endoreplication was observed only in a minor fraction of the hybrid’s gonocytes, while the vast majority of gonocytes were unable to duplicate their genomes and consequently could not proceed beyond pachytene due to defects in bivalent formation. We also noted that the rate of endoreplication was significantly higher among gonocytes of hybrids from natural clones than of experimentally produced F1 hybrids. Thus, asexuality and hybrid sterility are intimately related phenomena and the transition from sexual reproduction to asexuality must overcome significant problems with genome incompatibilities with a possible impact on reproductive potential.

show abstract

Complications dawn for kinetochore regulation by Aurora

Cited by 5 publications

References 17 publications

A Cyclin A—Myb-MuvB—Aurora B network regulates the choice between mitotic cycles and polyploid endoreplication cycles

A Cyclin A—Myb-MuvB—Aurora B network regulates the choice between mitotic cycles and polyploid endoreplication cycles

Challenges and costs of asexuality: Variation in premeiotic genome duplication in gynogenetic hybrids from Cobitis taenia complex

Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from Cobitis taenia Complex

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