Components of yeast (Sacchromyces cervisiae) extract as defined media additives that support the growth and productivity of CHO cells

Spearman, Maureen; Chan, Sarah; Jung, Vince; Kowbel, V.; Mendoza, M. S. Carrasco de; Miranda, V.; Butler, Michael

doi:10.1016/j.jbiotec.2016.04.031

Cited by 22 publications

(7 citation statements)

References 69 publications

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“…In CHO cell culture medium for expressing another antibody drug, Herceptin, the addition of yeast lysate led to a production titer as high as 1265 mg L −1 , compared to 760 mg L −1 in the control group. [74] Spearman et al [52] showed that bioactive components derived from yeast lysates with a molecular weight cutoff (MWCO) of 3 kDa effectively supported long-term passaging of CHO cells. Further analysis using cation exchange chromatography and metabolomics identified two significant polyamines, spermine and spermidine, which enhanced cell growth and increased production.…”

Section: Cell-free Supernatantsmentioning

confidence: 99%

Advances in serum‐free media for CHO cells: From traditional serum substitutes to microbial‐derived substances

Zhang,

Zhao,

et al. 2024

Biotechnology Journal

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The Chinese hamster ovary (CHO) cell is an epithelial‐like cell that produces proteins with post‐translational modifications similar to human glycosylation. It is widely used in the production of recombinant therapeutic proteins and monoclonal antibodies. Culturing CHO cells typically requires the addition of a certain proportion of fetal bovine serum (FBS) to maintain cell proliferation and passaging. However, serum is characterized by its complex composition, batch‐to‐batch variability, high cost, and potential risk of exogenous contaminants such as mycoplasma and viruses, which impact the purity and safety of the synthesized proteins. Therefore, search for serum alternatives and development of serum‐free media for CHO‐based protein biomanufacturing are of great significance. This review systematically summarizes the application advantages of CHO cells and strategies for high‐density expression. It highlights the developmental trends of serum substitutes from human platelet lysates to animal‐free extracts and microbial‐derived substances and elucidates the mechanisms by which these substitutes enhance CHO cell culture performance and recombinant protein production, aiming to provide theoretical guidance for exploring novel serum alternatives and developing serum‐free media for CHO cells.

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Section: Cell-free Supernatantsmentioning

confidence: 99%

Advances in serum‐free media for CHO cells: From traditional serum substitutes to microbial‐derived substances

Zhang,

Zhao,

et al. 2024

Biotechnology Journal

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“…cytokines, including growth factors such as FGF-2 and TGF-β, and hormones such as insulin are responsible for the control of cell proliferation and differentiation, and may represent the most costly media components (Guan et al, 2021;Jairath et al, 2021;O'Neill et al, 2021). Alternative ingredients, such as yeast and microalgae extracts, have shown progress for mammalian cell medium supplementation (Okamoto et al, 2020;Spearman et al, 2016).…”

Section: Cell Expansion and Differentiation For Cultivated Meat Productionmentioning

confidence: 99%

Cultivated meat: recent technological developments, current market and future challenges

Letti¹,

Karp²,

Molento³

et al. 2021

Biotechnology Research and Innovation

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“…The presence of ornithine in yeast extract is of particular interest due to its role as a precursor to the polyamines (putrescine, spermidine, spermine), which have been shown to stimulate cell growth and proliferation in a variety of cell lines. [35,36] Lactate, F I G U R E 6 Proposed simplified mechanism for the acid hydrolysis decomposition of a-GPC sarcosine, and 4-aminobutyrate appear to have an overall decreasing trend, but no definitive claims can be made due to quantification variability. Succinate, betaine, and citrate appear stable throughout the 12-hour acid digestion.…”

Section: Organic Acids Vitamins Micronutrientsmentioning

confidence: 99%

Enhanced characterization of yeast hydrolysate combining acid digestion and 1D‐1H NMR targeted profiling

2021

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Yeast extract, or autolysate, is a required component for many cell-culture media, but its exact constituents and benefits are unknown. Yeast extract contains a diverse assortment of metabolites, often present in complex forms (eg, polypeptides and polynucleotides). This study employs one-dimensional proton nuclear magnetic resonance (1D-1H NMR) spectroscopy to analyze free (ie, readily available) components present in commercially available yeast autolysate. The product is monitored while further subjected to acid hydrolysis, allowing for a more robust understanding of the exact components present, particularly those contained in complex forms. The amino acids and glucose compounds behaved as expected based on other acid hydrolysis studies, and were modelled similarly. Parameter estimation was in strong agreement with pre-hydrolysis targeted 1D-1H NMR profiling. This analysis was expanded to components not as thoroughly investigated and was especially applicable to nucleic compounds. Acid hydrolysis revealed that the yeast extract was approximately 5.4% nucleic material by weight, mostly composed of adenosine, and largely provided by RNA due to the presence of uracil and lack of thymidine. Choline-containing compounds were also positively identified with an observable increase of free choline during hydrolysis. 1D-1H NMR spectroscopy allowed for the simultaneous monitoring of a significant number of yeastextract metabolites, some of which were previously unreported. Utilizing 1D-1H NMR spectroscopy in conjunction with acid hydrolysis led to a more complete view of the compounds present, and accounted for an additional 24% of the yeast-extract mass.

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Components of yeast (Sacchromyces cervisiae) extract as defined media additives that support the growth and productivity of CHO cells

Cited by 22 publications

References 69 publications

Advances in serum‐free media for CHO cells: From traditional serum substitutes to microbial‐derived substances

Advances in serum‐free media for CHO cells: From traditional serum substitutes to microbial‐derived substances

Cultivated meat: recent technological developments, current market and future challenges

Enhanced characterization of yeast hydrolysate combining acid digestion and 1D‐1H NMR targeted profiling

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