Composite Cellulose Fibre for Affinity Chromatography Application

Abstract

Affinity chromatographic supports are nowadays one of the most frequently used and expensive consumable materials for protein purification at the laboratory and industrial scales. The introduction of cost-effective materials is an important issue to address in order to spread the usefulness of this technology. Cotton fibres are a highly available natural material with excellent mechanical and structural properties, which can be used for this purpose. Nevertheless, fibre insolubility and low chemical reactivity are the major drawbacks preventing the use of this material for protein chromatography. In this work, a composite prepared from polymethacrylate/cellulose fibres was used for the preparation of chromatographic materials containing immobilised proteins as adsorptive ligands. A green fluorescent protein and a protein A-similar ligand, containing cysteine in the terminal tags, were immobilised onto the fibres by epoxy-thiol chemistry. Buffer salt, pH, reaction time and pre-swelling procedure were optimised. The materials were characterised by fluorescence and electron microscopy, in addition to the binding capacity of the adsorbent materials.

Oxidative Polymerization of Aniline on the Surface of Sisal Fibers (SFs) as Defluoridation Media for Groundwater

“…The last stage beyond 450°C inferred the degradation of polyaniline, which is absent in the sisal fiber (line A). Introduction of polyaniline further proved the enhancement of thermal stability of sisal fiber [ 25 , 29 ].…”

Perspectives on adsorption technology as an effective strategy for continuous downstream bioprocessing

“…The decent mechanical arrangement of the composite fibres can result in good convective mass flows 39 . Well‐designed functional group conjugation can result in low‐cost alternative materials to prepare disposable devices for immunoglobulin purification 40 . The limitation of some types of fibrous adsorbent systems is a relatively small surface area (~10 m 2 g –1 ), 41 mainly governed by the fibre diameter comparing to bead‐based chromatographic adsorbents (~50 m 2 g –1 ) 42 and/or limited reactive sites for bulk bioproducts.…”

Moving adsorption belt system for continuous bioproduct recovery utilizing composite fibrous adsorbents

“…For example, Gavara et al (2012) proposed the utilization of composite adsorbent fibers for protein chromatography. Well-designed functional group conjugation onto fibers resulted in disposable devices for immunoglobulin purification ( Carbajal et al, 2020 ). Additionally, fibrous materials are known for their high voidage, low pressure drops ( Zhang et al, 2019 ), biocompatibility ( Ying et al, 2007 ), and scale-up potential ( Ghosh, 2002 ).…”