Abstract-TCC consists of timber and concrete members acting compositely together. The strength, stiffness, location and number of connectors play a crucial role for the composite action and structural performance of TCCs.In upgrading of timber floor to TCC, existing floorboards can be used as a permanent formwork whilst the concrete is then poured over connectors inserted into timber floorboards. Hence, the influence of interlayer on mechanical properties of TCC joints and floor is of interest.To date, conventional concrete has been used in most investigations on TCCs. Self-compacting concrete (SCC) is recognized as a highly workable which is compacted without use of conventional vibration methods. As such, there is a potential for application of SCC in TCCs where the pumping, and compacting of conventional concrete poses a challenge e.g. difficult to access areas in the renovation of timber floors in multi-story buildings. This paper focuses on utilizing mechanical fasteners for their ductility and stiffness to connect a SCC slab (with and without interlayer) to a timber beam, that is, the use of SCC forms an important parameter of this research. The experiment consists of push-out tests to characterizeload capacity and various stiffness. The responses of the specimens are also compared to that of TCCs with conventional concrete.Index Terms-Timber concrete composite (TCC), interlayer, self-compacting concrete (SCC), SFS screw connection.