Composite ECM–alginate microfibers produced by microfluidics as scaffolds with biomineralization potential

Angelozzi, Marco; Miotto, Martina; Penolazzi, Letizia; Mazzitelli, Stefania; Keane, Timothy J.; Badylak, Stephen F.; Piva, Roberta; Nastruzzi, Claudio

doi:10.1016/j.msec.2015.06.004

Cited by 38 publications

(34 citation statements)

References 34 publications

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“…With respect to the concentrations of biomaterial used for the preparation of microfibers (sodium alginate, gelatin, and UBM), the selected amounts were chosen on the basis of a large number of previously published observations (Penolazzi et al, 2010, 2012; Mazzitelli et al, 2013; Angelozzi et al, 2015; Vecchiatini et al, 2015). For instance, alginate is typically used in the concentration range comprised between 0.5 and 2.5% (w/v) depending on the stiffness required by the specific application of the gel.…”

Section: Resultsmentioning

confidence: 99%

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Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair

Angelozzi

Penolazzi

Mazzitelli

et al. 2017

Front. Bioeng. Biotechnol.

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Tissue engineering (TE) approaches using biomaterials have gain important roles in the regeneration of cartilage. This paper describes the production by microfluidics of alginate-based microfibers containing both extracellular matrix (ECM)-derived biomaterials and chondrocytes. As ECM components gelatin or decellularized urinary bladder matrix (UBM) were investigated. The effectiveness of the composite microfibers has been tested to modulate the behavior and redifferentiation of dedifferentiated chondrocytes. The complete redifferentiation, at the single-cell level, of the chondrocytes, without cell aggregate formation, was observed after 14 days of cell culture. Specific chondrogenic markers and high cellular secretory activity was observed in embedded cells. Notably, no sign of collagen type 10 deposition was determined. The obtained data suggest that dedifferentiated chondrocytes regain a functional chondrocyte phenotype when embedded in appropriate 3D scaffold based on alginate plus gelatin or UBM. The proposed scaffolds are indeed valuable to form a cellular microenvironment mimicking the in vivo ECM, opening the way to their use in cartilage TE.

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Section: Resultsmentioning

confidence: 99%

“…Particularly, composite microfibers (i.e., 3D scaffolds), potentially suitable for a fiber-based tissue such as cartilage, have been designed and produced by a specific microfluidic approach (Angelozzi et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair

Angelozzi

Penolazzi

Mazzitelli

et al. 2017

Front. Bioeng. Biotechnol.

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“…Alginate is the most commonly used hydrogel for bioprinting due to its low cost, biocompatible nature, high viscosity, and fast gelation kinetics. In bone bioprinting, alginate has been used alone or in combination with other biomaterials ( Table 2 ). However, alginate suffers from low bioactivity, and thus, other polymers that have better bioactivity such as collagen were explored.…”

Section: Bone Biofabrication Parameters and Requirementsmentioning

confidence: 99%

“…Chemical crosslinking can also be achieved, e.g., by the use of UV light for GelMA . Physical crosslinking approaches include thermal gelation for collagen and sonication for silk fibroin, and ionic gelation using calcium, barium or strontium for alginate. Many of these methods can affect cell viability, and thus, their use and exposure time should be carefully adjusted.…”

Section: Bone Biofabrication Parameters and Requirementsmentioning

confidence: 99%

“…For instance, the use of two inlet snake micromixing chips was found to achieve a homogeneous distribution of cells in printed microfibers. In one study, microfluidic microfiber bone bioprinting was achieved by using a hydrogel made of either alginate with a gelatin solution or alginate with a particulate urinary bladder matrix (UBM) and SaOS‐2 cells . In another study, alginate fibers were bioprinted by using microfluidic channels containing CaCl 2 solution as a crosslinking agent, and the bioprinted fibers were pulled on a roller.…”

Section: Bioprinted Bone Constructsmentioning

confidence: 99%

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Advancing Frontiers in Bone Bioprinting

Ashammakhi

Hasan

Kaarela

et al. 2019

Adv Healthcare Materials

189

182

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Three‐dimensional (3D) bioprinting of cell‐laden biomaterials is used to fabricate constructs that can mimic the structure of native tissues. The main techniques used for 3D bioprinting include microextrusion, inkjet, and laser‐assisted bioprinting. Bioinks used for bone bioprinting include hydrogels loaded with bioactive ceramics, cells, and growth factors. In this review, a critical overview of the recent literature on various types of bioinks used for bone bioprinting is presented. Major challenges, such as the vascularity, clinically relevant size, and mechanical properties of 3D printed structures, that need to be addressed to successfully use the technology in clinical settings, are discussed. Emerging approaches to solve these problems are reviewed, and future strategies to design customized 3D printed structures are proposed.

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Multifunctional Micro/Nanoscale Fibers Based on Microfluidic Spinning Technology

2019

Advanced Materials

193

174

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Superfine multifunctional micro/nanoscale fibrous materials with high surface area and ordered structure have attracted intensive attention for widespread applications in recent years. Microfluidic spinning technology (MST) has emerged as a powerful and versatile platform because of its various advantages such as high surface‐area‐to‐volume ratio, effective heat transfer, and enhanced reaction rate. The resultant well‐defined micro/nanoscale fibers exhibit controllable compositions, advanced structures, and new physical/chemical properties. The latest developments and achievements in microfluidic spun fiber materials are summarized in terms of the underlying preparation principles, geometric configurations, and functionalization. Variously architected structures and shapes by MST, including cylindrical, grooved, flat, anisotropic, hollow, core–shell, Janus, heterogeneous, helical, and knotted fibers, are emphasized. In particular, fiber‐spinning chemistry in MST for achieving functionalization of fiber materials by in situ chemical reactions inside fibers is introduced. Additionally, the applications of the fabricated functional fibers are highlighted in sensors, microactuators, photoelectric devices, flexible electronics, tissue engineering, drug delivery, and water collection. Finally, recent progress, challenges, and future perspectives are discussed.

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Composite ECM–alginate microfibers produced by microfluidics as scaffolds with biomineralization potential

Cited by 38 publications

References 34 publications

Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair

Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair

Advancing Frontiers in Bone Bioprinting

Multifunctional Micro/Nanoscale Fibers Based on Microfluidic Spinning Technology

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