Composite electrospun nanomembranes of fish scale collagen peptides/chito-oligosaccharides: antibacterial properties and potential for wound dressing

Abstract

Purpose: The objective of the present investigation was to evaluate the antibacterial properties and the biocompatibility of composite electrospun nanofibrous membranes (NFMs) with low-molecular-weight fish scale collagen peptides (FSCP) and chito-oligosaccharide (COS), to determine their potential for use as wound dressings. Methods: Low-molecular-weight FSCP were combined with COS to prepare nanofibers by electrospinning, and polyvinyl alcohol (PVA) was used for enhancing fiber-forming ability. Transmission electron microscope and scanning electron microscope methods were used to observe bacterial adhesion and the bacterial cell membrane. Fibroblast cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The best FSCP/COS mass ratio for electrospinning was 2:1, and the nanofibers had small dimensions ranging from 50 to 100 nm. The NFM showed good antibacterial activities against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The antibacterial activity against S. aureus was higher than against E. coli . The pili and adhesive fimbriae of E. coli promoted bacterial adhesion to the NFM surfaces, and S. aureus biofilms aided S. aureus adhesion on the surface of NFMs. Damage to the bacterial cell membrane indicates that the NFMs could lead to the release of intracellular materials, particularly with S. aureus . In addition, FSCP/COS NFM rapidly increased the permeability of the outer membranes of E. coli . The electrospun NFM with FSCP and COS had good biocompatibility in vitro and supported proliferation of human skin fibroblasts. Conclusion: FSCP are superior to mammalian collagen, and have feasibility and potency for wound dressings. FSCP/COS NFMs had good anti-bactericidal activity that improved with increased COS, and showed good biocompatibility in vitro and supported the proliferation of fibroblasts.

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

“…Moreover, the tilapia collagen nanofibers accelerated wound healing in vivo in rat models [91][92][93][94]. Beneficial effects on wound healing were also observed in nanofibrous meshes electrospun from collagen obtained from Arothron stellatus [96] and from fish scale collagen peptides [90].…”

“…In addition, collagen has been reported to be relatively poorly immunogenic, even if used in allogeneic and xenogeneic forms, for example, recombinant human collagen or bovine and porcine collagen. However, mammalian collagen is associated with the risk of disease transmission, for example, bovine spongiform encephalopathy (for a review, see [89][90][91]). This risk can be reduced by the use of fish collagen, which became to be popular in tissue engineering, including skin tissue engineering and wound healing.…”

Marine Collagen as A Promising Biomaterial for Biomedical Applications

“…This collagen sponge showed excellent biocompatibility in the culture of mouse fibroblasts (NIH3T3) and human keratinocytes (HaCaT), suggesting that the sponge may be a potential candidate for the burn/wound dressing material. A composite electrospun nanomembrane of MCP/polyvinyl alcohol (PVA)/chito-oligosaccharides not only showed good anti-bacterial activities against Staphylococcus aureus and Escherichia coli , but also showed good biocompatibility in vitro and supported the proliferation of human skin fibroblasts, indicating its potential application in wound dressings [125].…”

“…Good antibacterial activities against Staphylococcus aureus and Escherichia coli 2. Supported fibroblast proliferation[125]Collagen peptideEnzymatical hydrolysisCommercially available fish type I collagen hydrolysate from Amino collagen (Meiji Seika, Tokyo, Japan)6 weeks clinical studies in 25 Japanese women volunteers (35.1 ± 5.4 years old)Improved skin hydration[126,127]Collagen peptideEnzymatical hydrolysisMCP stabilized orthosilicic acidRandomized patient groups1. No side effects, hypersensitivity, or systemic symptoms2.…”

Marine-Derived Collagen as Biomaterials for Human Health

“…The healing experiments on rat skin wounds also showed the ability of MDC to promote wound healing ( 35 , 36 ). At the same time, MDC is also an excellent scaffold for skin tissue regeneration ( 79 ), and a potential wound dressing with antimicrobial properties ( 115 ).…”