The article presents an algorithm for assessing the business activity of the region as an objective quantitative indicator of processes in the regional system. This algorithm considers the effect of the main regional complex subsystems: resources, production, innovation, society, entrepreneurship, finance, investment, information, infrastructure, environmental subsystem, which is reflected in the model of business activity formation in the region. The business activity model of the region includes 10 blocks that characterize the mechanisms of the regional complex functioning. The formed system of evaluation indicators reflects various aspects of the regional complex business activity. The formalization of the complex indicator of regional business activity is carried out in the form of a function that determines the type of mathematical model, considering the justified blocks of region business activity and considering the impact of factors and the results of its functioning on the regional complex. When developing an algorithm for assessing the region business activity as an indicator of socio-economic processes, a sequence of calculation procedures is justified, which allows identifying both basic and chain indicators of the business activity of the region for management purposes. The presented algorithm makes it possible to assess the dynamics of the region business activity level on the basis of objective statistically significant information, as well as to determine the features of the processes taking place in the regional complex that have a different impact on the efficiency level of the region. The practical use of the proposed algorithm can be a source of objective information for the development of solutions at the regional management level, considering the specific factors of a particular region functioning, which is provided by the procedure of the algorithm for assessing the business activity of the region.