Composite inclusion complexes containing hyaluronic acid/chitosan nanosystems for dual responsive enrofloxacin release

Liu, Yuda; Chen, Dongmei; Zhang, Aoxue; Xiao, Mi; Li, Zhenxia; Luo, Wanhe; Pan, Yuanhu; Qu, Wei; Xie, Shuyu

doi:10.1016/j.carbpol.2020.117162

Cited by 43 publications

(34 citation statements)

References 27 publications

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“…On the other hand, the particle sizes measured by SEM were smaller than the genuine diameters. This result is consistent with previous studies [ 5 ].…”

Section: Discussionsupporting

confidence: 95%

“…The viable colonies were then counted after 24 h. Each concentration was performed in triplicate. The limit of detection was 10 CFU/mL [ 5 ].…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, tilmicosin might be ineffective for SASCVs mastitis because of its inadequate therapeutic drug concentrations and insufficient residence time of the antibiotic in the mammary gland [ 4 ]. The therapeutic drug concentrations and residence time in the mammary gland could be increased by modifying the bioadhesive ability of tilmicosin to SASCVs [ 5 ]. Therefore, there is an urgent need to develop novel smart delivery systems to modify the bioadhesive ability of tilmicosin to SASCVs, increase the therapeutic drug concentrations and residence time in the mammary gland, and enhance the efficacy of tilmicosin against cow mastitis caused by SASCVs.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels

et al. 2022

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Background The poor bioadhesion capacity of tilmicosin resulting in treatment failure for Staphylococcus aureus small colony variants (SASCVs) mastitis. Objectives This study aimed to increase the bioadhesion capacity of tilmicosin for the SASCVs strain and improve the antibacterial effect of tilmicosin against cow mastitis caused by the SASCVs strain. Methods Tilmicosin-loaded chitosan oligosaccharide (COS)-sodium carboxymethyl cellulose (CMC) composite nanogels were formulated by an electrostatic interaction between COS (positive charge) and CMC (negative charge) using sodium tripolyphosphate (TPP) (ionic crosslinkers). The formation mechanism, structural characteristics, bioadhesion, and antibacterial activity of tilmicosin composite nanogels were studied systematically. Results The optimized formulation was comprised of 50 mg/mL (COS), 32 mg/mL (CMC), and 0.25 mg/mL (TPP). The size, encapsulation efficiency, loading capacity, polydispersity index, and zeta potential of the optimized tilmicosin composite nanogels were 357.4 ± 2.6 nm, 65.4 ± 0.4%, 21.9 ± 0.4%, 0.11 ± 0.01, and -37.1 ± 0.4 mV, respectively; the sedimentation rate was one. Scanning electron microscopy showed that tilmicosin might be incorporated in nano-sized crosslinked polymeric networks. Moreover, adhesive studies suggested that tilmicosin composite nanogels could enhance the bioadhesion capacity of tilmicosin for the SASCVs strain. The inhibition zone of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels were 2.13 ± 0.07, 3.35 ± 0.11, and 1.46 ± 0.04 cm, respectively. The minimum inhibitory concentration of native tilmicosin, tilmicosin standard, and tilmicosin composite nanogels against the SASCVs strain were 2, 1, and 1 µg/mL, respectively. The in vitro time-killing curves showed that the tilmicosin composite nanogels increased the antibacterial activity against the SASCVs strain. Conclusions This study provides a potential strategy for developing tilmicosin composite nanogels to treat cow mastitis caused by the SASCVs strain.

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“…On the other hand, the particle sizes measured by SEM were smaller than the genuine diameters. This result is consistent with previous studies [ 5 ].…”

Section: Discussionsupporting

confidence: 95%

“…The viable colonies were then counted after 24 h. Each concentration was performed in triplicate. The limit of detection was 10 CFU/mL [ 5 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels

et al. 2022

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“…Liu et al [ 9 ] reported an increase in the enrofloxacin concentration in S. aureus biofilms by enhancing the bioadhesion ability of antibacterial agents. Thus, the intracellular therapeutic drug concentrations might be increased by modifying the bioadhesive ability of antibacterial agents to bacteria.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants

et al. 2022

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Background The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs). Objectives In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs. Methods Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl 2 (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes. The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically. Results The optimized formulation was comprised of 10 mg/mL (gelatin), 5 mg/mL (SA), and 0.25 mg/mL (CaCl 2 ). The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and −34.4 ± 0.8 mV, respectively. Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions. In addition, the enrofloxacin composite nanogels could enhance the bioadhesion capacity of enrofloxacin for the SASCVs strain by adhesive studies. The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 μg/mL, respectively. The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects. Conclusions This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.

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“…The available amine groups of CHT can electrostatically interact with the anionic mucin from mucous tissues, increasing the CHT residence time [ 13 ]. However, CHT is sensitive to acid media, such as the gastric microenvironment [ 14 ]. On the other hand, xanthan gum (XAN) is an anionic exopolysaccharide secreted by Xanthomonas campestris [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Nanobeads for Oral Indomethacin Delivery

et al. 2022

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The oral administration of the anti-inflammatory indomethacin (INDO) causes severe gastrointestinal side effects, which are intensified in chronic inflammatory conditions when a continuous treatment is mandatory. The development of hybrid delivery systems associates the benefits of different (nano) carriers in a single system, designed to improve the efficacy and/or minimize the toxicity of drugs. This work describes the preparation of hybrid nanobeads composed of nanostructured lipid carriers (NLC) loading INDO (2%; w/v) and chitosan, coated by xanthan. NLC formulations were monitored in a long-term stability study (25 °C). After one year, they showed suitable physicochemical properties (size < 250 nm, polydispersity < 0.2, zeta potential of −30 mV and spherical morphology) and an INDO encapsulation efficiency of 99%. The hybrid (lipid-biopolymers) nanobeads exhibited excellent compatibility between the biomaterials, as revealed by structural and thermodynamic properties, monodisperse size distribution, desirable in vitro water uptake and prolonged in vitro INDO release (26 h). The in vivo safety of hybrid nanobeads was confirmed by the chicken embryo (CE) toxicity test, considering the embryos viability, weights of CE and annexes and changes in the biochemical markers. The results point out a safe gastro-resistant pharmaceutical form for further efficacy assays.

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Composite inclusion complexes containing hyaluronic acid/chitosan nanosystems for dual responsive enrofloxacin release

Cited by 43 publications

References 27 publications

Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels

Enhanced antibacterial activity of tilmicosin against Staphylococcus aureus small colony variants by chitosan oligosaccharide-sodium carboxymethyl cellulose composite nanogels

Antibacterial activity of enrofloxacin loaded gelatin-sodium alginate composite nanogels against intracellular Staphylococcus aureus small colony variants

Hybrid Nanobeads for Oral Indomethacin Delivery

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