Composite Porous Tubular Biopolymer Matrix of Small Diameter

Немец, Е. А.; Belov, V. Yu.; Ilina, T. S.; Surguchenko, V. A.; Pankina, A. P.; Sevastianov, V. I.

doi:10.1134/s207511331902031x

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…ИНП в виде трубок с внутренним диаметром 2 мм (рис. 1) изготавливали методом электроспиннинга, разработанным ранее для создания тканеинженерных конструкций кровеносных сосудов малого диаметра [17], из 10% (w/w) раствора поликапролактона (ПКЛ, ММ 80000, Sigma-Aldrich, США, образец № 1), ПКЛ с добавлением желатина (Sigma-Aldrich, США, образец № 2) и ПКЛ с добавлением коллагена (Коллост, Россия, образец № 3) в гексафторизопропаноле (АО НПО «ПИМ-ИНВЕСТ», Россия) на установке для электроспиннинга NANON-01A («MECC C O », Япония) при напряжении между электродами 25 кВ, скорости подачи раствора 4 мл/ч, расстоянии до коллектора 100 мм, скорости вращения стержня-подложки 1000 об/мин, с использованием иглы 18 G. После окончания процесса нанесения раствора полученные образцы сушили в термостате при температуре 37 °С в течение 2 ч с последующим вакуумированием для удаления следов растворителя при остаточном давлении 10-20 мм рт. ст.…”

Section: материал и методыunclassified

Artificial nerve conduit for guiding peripheral nerve growth (cadaveric study)

Fedyakov,

Nemets,

Dreval

et al. 2023

RJTAO

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At present, the search for effective ways of restoring peripheral nerves with anatomical damage continues. Autoplasty still remains the gold standard, which, however, is not without its drawbacks. The use of nerve implants for promoting directional axon growth is essential and promising.Objective: to study the biomechanical properties of laboratory samples of an artificial nerve conduit (NGC) made of hybrid biomaterials and to, on cadaveric material, assess the technical feasibility of using them in surgical practice to repair extended peripheral nerve defects.Material and methods. The objects of the study were three electrospun NGC samples: from synthetic material (polycaprolactone, PCL) and hybrid biomaterials (PCL + gelatin or PCL + collagen). The work compared the physical and mechanical properties of NGC: stiffness, plasticity, elasticity, brittleness, resistance to chemical attack, their ability to be impregnated with liquid media, permeability, possibility of making an anastomosis between the implant and the nerve during surgical procedure. Cadaveric material was the object of the study: we used a dissected superficial sensory branch of the human right radial nerve, 2 mm in diameter, isolated on the forearm, about 12 cm in length, because it most corresponded to the diameter of the NGC samples tested. After surgery, the echogenic features of the implants and their anastomoses with the nerve were assessed by ultrasound imaging.Results. It was found that hybrid NGC samples, based on their biomechanical properties, are fundamentally suitable for use in surgical practice, to ensure growth and replacement of a peripheral nerve defect. However, the best composition of a nerve guide can be established after comparative preclinical study of the biocompatible and functional properties of hybrid material samples.Conclusion. The physical and mechanical properties of the investigated NGC samples made of hybrid biomaterials meet the technical requirements for implantable nerve conduits for surgical application.

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Section: материал и методыunclassified

Artificial nerve conduit for guiding peripheral nerve growth (cadaveric study)

Fedyakov,

Nemets,

Dreval

et al. 2023

RJTAO

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“…Жела-тин, являющийся продуктом денатурации коллагена, из которого наиболее часто формируют матрицы в виде губок, сеток и гидрогелей [4,5], не только менее иммуногенен, но и подобно коллагену содержит аминокислотную последовательность Arg-Gly-Asp (RGD), обусловливающую его адгезивные свойства [6,7]. Носители лекарственных веществ и клеток на основе желатина или в комбинации с другими природными или синтетическими полимерами достаточно широко применяются в различной форме, включая капсулы и микрокапсулы, микро-и наночастицы, микро-и нановолокна, гидрогели [8][9][10].…”

Section: Introductionunclassified

Cryogenically structured gelatin-based hydrogel as a resorbable macroporous matrix for biomedical technologies

Grigoriev

Basok

Kirillova

et al. 2022

RJTAO

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Objective: to investigate the biological properties of a matrix made of cryogenically structured hydrogel in the form of a macroporous gelatin sponge, as well as the possibility of creating cell-engineered constructs (CECs) on its basis. Materials and methods. The main components of the cryogenically structured hydrogel were gelatin (type A) obtained from porcine skin collagen, N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide, (EDC) and urea (all from Sigma-Aldrich, USA). Surface morphology was examined using scanning electron microscopy (SEM). The degree of swelling in water of the samples was determined by gravimetric method. Cytotoxicity was studied on NIH3T3, a fibroblast cell line isolated from a mouse, and on human adipose-derived mesenchymal stem/stromal cells (hAMSCs) using IncuCyte ZOOM (EssenBioscience, USA). The metabolic activity of hAMSCs was assessed using PrestoBlue™ reagents (Invitrogen™, USA). To create CECs, we used hAMSCs, human hepatocellular carcinoma cell line HepG2 or human umbilical vein endothelial cell lines EA.hy926. Albumin content in the culture medium was determined by enzyme immunoassay. Ammonia metabolism rate was assessed after 90 minutes of incubation with 1 mM ammonium chloride (Sigma-Aldrich, USA) diluted in a culture medium on day 15 of the experiment. Results. Obtaining a cryogenically structured hydrogel scaffold in the form of macroporous gelatin sponge included freezing an aqueous solution of a gelatin+urea mixture, removal of polycrystals of frozen solvent by lyophilization, extraction of urea with ethanol and treatment of the cryostructurate with an ethanol solution of EDC. Scanning electron microscopy identified three types of pores on the carrier surface: large (109 ± 17 μm), medium (39 ± 10 μm), and small (16 ± 6 μm). The degree of swelling in water of the matrix samples was 3.8 ± 0.2 g H2O per 1 g of dry polymer. The macroporous gelatin sponge as a part of CEC was found to have the ability to support adhesion and proliferation of hAMSCs, EA.hy926 and HepG2 for 28, 15 and 9 days, respectively. Albumin secretion and ammonia metabolism when HepG2 cells were cultured on the gelatin sponge were detected. Conclusion. The use of a matrix made from macroporous cryogenically structured gelatin-based hydrogel for tissue engineering products is shown to be promising using a cell-engineered liver construct as a case.

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