Composite Scaffolds of Gelatin and Fe₃O₄ Nanoparticles for Magnetic Hyperthermia‐Based Breast Cancer Treatment and Adipose Tissue Regeneration

Abstract

Postsurgical treatment of breast cancer remains a challenge with regard to killing residual cancer cells and regenerating breast defects. To prepare composite scaffolds for postoperative use, gelatin is chemically modified with folic acid (FA) and used for hybridization with citrate‐modified Fe3O4 nanoparticles (Fe3O4‐citrate NPs) to fabricate Fe3O4/gelatin composite scaffolds which pore structures are controlled by free ice microparticles. The composite scaffolds have large spherical pores that are interconnected to facilitate cell entry and exit. The FA‐functionalized composite scaffolds have the ability to capture breast cancer cells. The Fe3O4/gelatin composite scaffolds possess a high capacity for magnetic‐thermal conversion to ablate breast cancer cells during alternating magnetic field (AMF) irradiation. In addition, the composite scaffolds facilitate the growth and adipogenesis of mesenchymal stem cells. The composite scaffolds have multiple functions for eradication of residual cancer cells under AMF irradiation and for regeneration of resected adipose tissue when AMF is off.

Breast Cancer Treatment Using the Magneto-Hyperthermia Technique Associated with Omega-3 Polyunsaturated Fatty Acids’ Supplementation and Physical Training

“…Its pathophysiology presents a series of challenges, including the need to identify new prevention and treatment strategies, aiming to improve patients’ quality of life affected by this disease [ 46 , 47 , 48 , 49 ]. Recent studies have provided promising evidence of the benefits of omega-3 supplementation and complementary therapies in combination with chemotherapy, radiotherapy, and surgical procedures for patients with BC [ 50 , 51 , 52 , 53 ].…”

Effect of Hydrogel Stiffness on Chemoresistance of Breast Cancer Cells in 3D Culture

“…The P-glycoprotein mRNA expression levels in the cells/agarose and cells/agarose/DOX constructs were analyzed after cell culturing for 36 h using real-time PCR according to a previous method [ 55 , 56 ]. The samples were immersed in 1 mL of a Sepasol solution (Nacalai Tesque, Kyoto, Japan) for extraction of total RNA.…”

Biomedical Application of Decellularized Scaffolds

“…The usual treatment is removal of a breast tumor or breast. This can cause serious harm to the patient’s mentality. − Therefore, breast reconstruction is particularly important . Liu et al prepared decellularized breast scaffolds by decellularizing human breast cancer biopsy specimens with sodium lauryl ether sodium sulfate (SLES) solution, and made a certain contribution to the effective treatment of breast cancer in the future …”