Composite scaffolds of nano-hydroxyapatite and silk fibroin enhance mesenchymal stem cell-based bone regeneration via the interleukin 1 alpha autocrine/paracrine signaling loop

Liu, Hua; Xu, Guangyi; Wang, Ya Fei; Zhao, Hong; Xiong, Sheng; Wu, Yan; Heng, Boon Chin; An, Cheng Rui; Zhu, Gang; Xie, Dongdong

doi:10.1016/j.biomaterials.2015.01.017

Cited by 135 publications

(85 citation statements)

References 37 publications

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“…Various materials were proposed for osteoinduction, such as inorganic ceramics, metals and polymers (Liu et al ., ; Tautzenberger et al ., ; Verket et al ., ; Zhang et al, ).…”

Section: Introductionmentioning

confidence: 99%

Enhancing bioactive properties of silk fibroin with diatom particles for bone tissue engineering applications

Liaudanskaya

Bonani

et al. 2017

J Tissue Eng Regen Med

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Many studies have highlighted the role of silicon in human bone formation and maintenance. Silicon, in fact, is considered to nucleate the precipitation of hydroxyapatite and to reduce the bone resorption. For this reason, we have combined silk fibroin (SF) with silicon-releasing diatom particles (DPs), as potential material for bone tissue engineering applications. Sponges of fibroin loaded with different amounts and sizes of DPs were prepared by solvent casting-particulate leaching method, and their morphology, porosity and mechanical properties were evaluated. The biological effect of diatom addition was assessed on human osteosarcoma cell line MG63, a suitable osteoblast-like model, through cell adhesion, metabolic activity and proliferation assays. In addition, alkaline phosphatase activity, osterix and collagen type I production in MG63 cell line were assessed as markers of early bone formation to demonstrate a pro-mineralization potential of scaffolds. Results of the studies showed that addition to fibroin of diatoms particles improved the osteogenic properties of osteoblast-like cells compared with the pure SF.

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“…Various materials were proposed for osteoinduction, such as inorganic ceramics, metals and polymers (Liu et al ., ; Tautzenberger et al ., ; Verket et al ., ; Zhang et al, ).…”

Section: Introductionmentioning

confidence: 99%

Enhancing bioactive properties of silk fibroin with diatom particles for bone tissue engineering applications

Liaudanskaya

Bonani

et al. 2017

J Tissue Eng Regen Med

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“…Specifically, silk based nanomaterials are investigated for bone and cartilage tissue regeneration [56,[86][87][88][89][90][91]. For example, silk-nHAp nanocomposites fabricated using electrospinning was used for controlled release of BMP2 [85].…”

mentioning

confidence: 99%

Nanoengineered biomaterials for repair and regeneration of orthopedic tissue interfaces

et al. 2016

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“…Iijima et al have reported that HA coating and synthetic HA discs suppressed the adipogenic differentiation of hMSCs through decreasing the formation of lipid droplets and the expression of PPARγ and C/EBPα. Similarly, Liu et al demonstrated that the mechanism of osteoinductivity of nHA coating on silk fibroin scaffolds could be inhibiting the adipogenesis via regulation of the PPAR signaling pathway. However, a previous study reported that the clodronate‐HA scaffold had no evidently inhibition of adipogenic differentiation of rat bone mesenchymal stromal cells .…”

Section: Discussionmentioning

confidence: 98%

“…According to the inverse relationship, HA has the potential to suppress adipogenesis. Several previous studies have shown that HA coating or HA discs can inhibit the adipogenesis of different cell types . For instance, Zhang et al found that HA discs composed of natural HA powder inhibited adipogenic differentiation of mouse bone marrow derived MSCs by regulating several microRNAs in the adipogenesis pathway.…”

Section: Discussionmentioning

confidence: 99%

The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells

Yang

Huang

et al. 2018

J Biomedical Materials Res

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Due to its excellent biocompatibility, nanosized hydroxyapatite (nHA) has drawn much attention for various applications in biomedical fields. There are growing concerns about its biosecurity; however, little is known about its effects on adipogenesis. In the present study, nHA with three different sizes were synthesized, and the in vitro effects of nHA on cell proliferation and adipogenic differentiation of human mesenchymal stem cells (hMSCs) were investigated. The results clearly show that nHA does not affect the cell viability, the lipids droplets formation, triglyceride (TG) synthesis, and the expression of adipogenic marker genes/proteins of hMSCs at concentrations lower than 50 μg/mL. It is concluded that the adipogenic differentiation potential of hMSCs is not affected by nHA at noncytotoxic concentrations. These will provide a reference for the applications of nHA in biomedical fields. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1822-1831, 2018.

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Composite scaffolds of nano-hydroxyapatite and silk fibroin enhance mesenchymal stem cell-based bone regeneration via the interleukin 1 alpha autocrine/paracrine signaling loop

Cited by 135 publications

References 37 publications

Enhancing bioactive properties of silk fibroin with diatom particles for bone tissue engineering applications

Enhancing bioactive properties of silk fibroin with diatom particles for bone tissue engineering applications

Nanoengineered biomaterials for repair and regeneration of orthopedic tissue interfaces

The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells

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