Composite whey protein–cellulose nanocrystals at oil-water interface: Towards delaying lipid digestion

Sarkar, Anwesha; Li, Hancong; Cray, Deborah; Boxall, Sally

doi:10.1016/j.foodhyd.2017.10.020

Cited by 114 publications

(63 citation statements)

References 36 publications

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“…In this direction of research, many authors have investigated manipulating the interface of droplets to restrict pepsinolysis of proteinaceous stabilizing layers by creating a more tortuous path for pepsin to reach the interface, for example by coating the adsorbed protein layer by layers of other material(s). In this fashion, gastric stability of emulsion droplets has been achieved by coating protein-stabilized droplets with a variety of non-digestible dietary fibres (Beysseriat, Decker, & McClements, 2006;Meshulam & Lesmes, 2014) and/or particles (Liu & Tang, 2016;Sarkar, Ademuyiwa, et al, 2018;Sarkar, Li, Cray, & Boxall, 2018;Sarkar, Zhang, Murray, Russell, & Boxal, 2017;Shao & Tang, 2016). The second strategy used in literature involves encapsulating emulsion droplets within a gel.…”

Section: Introductionmentioning

confidence: 99%

Overcoming in vitro gastric destabilisation of emulsion droplets using emulsion microgel particles for targeted intestinal release of fatty acids

2019

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Section: Introductionmentioning

confidence: 99%

Overcoming in vitro gastric destabilisation of emulsion droplets using emulsion microgel particles for targeted intestinal release of fatty acids

2019

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“…Hence, it might be useful to create a relatively complex interface to protect the emulsions against gastric destabilization or at least slow down the rate of hydrolysis of the interfacial material by pepsin. In this regard, recently, cellulose nanocrystals have shown success on enhancing the stability of whey protein-stabilized oil-in-water (O/W) emulsions against enzymatic attacks (Sarkar, Li, Cray, & Boxall, 2018 ;Sarkar, Zhang, Murray, Russell, & Boxal, 2017). Binding of CNC to the protein film at the interface offered resistance to the protein film against pepsinolysis and inhibited 5 droplet coalescence in the gastric phase that occurs typically in case of emulsions stabilized by protein film alone (Sarkar, Goh, Singh, & Singh, 2009b;Sarkar, et al, 2016a;Sarkar & Singh, 2016c;Sarkar, et al, 2017;Singh & Sarkar, 2011).…”

Section: Introductionmentioning

confidence: 99%

Pickering emulsions co-stabilized by composite protein/ polysaccharide particle-particle interfaces: Impact on in vitro gastric stability

et al. 2018

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The objective of this study was to delay the rate and extent of gastric destabilization of emulsions using composite particle-particle layers at the O/W interface. Pickering emulsions (20 wt% oil) were prepared using lactoferrin nanogel particles (LFN, Dh=100 nm) (1 wt%) or a composite layer of LFN and inulin nanoparticles, latter was enzymatically synthetized by inulosucrase IslA from Leuconostoc citreum (INP) (Dh=116±1 nm) (1 wt% LFN 3 wt% INP). The hypothesis was that creating a secondary layer of biopolymeric particles might act as a barrier to pepsin to access the underlying proteinaceous particles. Droplet size, microscopy (optical and transmission electron microscopy (TEM)),-potential and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to understand the colloidal fate of these Pickering emulsions in an in vitro gastric model (pH 3, 37 C, pepsin). The-potential measurements and TEM images confirmed that LFN and INP were at the O/W interface, owing to the electrostatic attraction between oppositely charged LFN (+29.3±0.7 mV) and INP (-10±1.8 mV) at both neutral and gastric pH. The SDS-PAGE results revealed that adsorbed LFN was less prone to pepsinolysis as compared to a typical protein monolayer at the interface. Presence of INP further decreased the rate and degree of hydrolysis of the LFN (>65% intact protein remaining after 60 min of digestion) by acting as a steric barrier to the diffusion of pepsin and inhibited droplet coalescence. Thus, composite particle-particle layers (LFN + INP) at droplet surface shows potential for rational designing of gastric-stable food and pharmaceutical applications.

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“…In the present study, bile acids and choline mixture supplementation, but not choline or bile acids alone, improved the growth performance of weaned piglets, re ecting as a marked higher ADG, a lower feed to gain ratio and a more body weight gain than control diet. The increased body weight gain and ADG by ChB was likely due to this mixture supplementation promoted dietary lipids digestion and absorption, re ecting as higher lipase activity in the jejunal digesta and higher concentration of total bile acids in the jejunum and ileum than those in control group [24,25]. Meanwhile, an augment of NEFA level and reduction in TG in the jejunal digesta further con rmed that ChB diet accelerated dietary lipids digestion.…”

Section: Discussionmentioning

confidence: 76%

Choline and Bile Acids Mixture Improved Growth Performance and Intestinal Immunity via Altering Gut Microbiota and Bacterial Metabolites in the Colon of Weaned Piglets.

Qiu

Liu

et al. 2020

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Background: Choline or bile acids has many beneficial roles in physiological function. However, little was known about growth performance, intestinal mucosal function and microbiota-host interactions of weaned piglets in response to choline or bile acids supplementation. This study aimed to investigate the effect of choline and bile acids mixtures (ChB) supplementation on growth performance, intestinal mucosal barrier function, gut microbiota and bacterial metabolites of weaned piglets. One hundred and twenty-eight crossbred (Duroc × Landrace × Large White) weaned piglets (initial body weight: approximately 8 kg; 21 d of age) were randomly allocated to four different dietary treatments(a control diet (Control) and the other three groups were control diet supplemented with 800 mg/kg choline chloride (choline), 500 mg/kg bile acids (bile acids) or 800 mg/kg choline chloride plus 500 mg/kg bile acids (ChB), respectively) and for 28-d feeding trail. Results: ChB significantly increased average daily gain (ADG) and reduced feed/gain (F/G) ratio, associated with elevation of lipase activity and total bile acids level in ileal digesta compared with control diet. Additionally, ChB altered colonic microbiota by increasing the relative abundance of Lactobacillus and Faecalibacterium , and decreasing the relative abundances of unidentified-Clostridiales , Parabacteroides and Unidentified-Ruminococcaceae , when compared with control diet. Meanwhile, ChB increased the butyrate level and decreased the production of bile acid profiles in the colonic digesta. Besides, feeding ChB improved gut immunity, as reflected by increasing the abundance of IL-10 , FXR and mucin2 transcript, while downregulated expression of TLR4 , MyD88 , NF-κBp65 and TNF-α genes in the intestinal mucosa. Quantitative proteomics of jejunal mucosa further showed that ChB regulated the proteins that were related to inflammatory response. Furthermore, the changes in the ADG and genes expression were associated with alteration of gut microbiota composition and their metabolites. Conclusions: Collectively, our findings demonstrated that choline and bile acids mixture may improve the growth performance and intestinal immune response of weaned piglets through alteration of gut microbiota composition and bacterial metabolites, which promoted gut health.

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Composite whey protein–cellulose nanocrystals at oil-water interface: Towards delaying lipid digestion

Abstract: 23Lipid digestion is an interfacial process that is largely controlled by the 24 adsorption of lipase + colipase + bile salts onto the surface of the emulsified lipid 25

Cited by 114 publications

References 36 publications

Overcoming in vitro gastric destabilisation of emulsion droplets using emulsion microgel particles for targeted intestinal release of fatty acids

Overcoming in vitro gastric destabilisation of emulsion droplets using emulsion microgel particles for targeted intestinal release of fatty acids

Pickering emulsions co-stabilized by composite protein/ polysaccharide particle-particle interfaces: Impact on in vitro gastric stability

Choline and Bile Acids Mixture Improved Growth Performance and Intestinal Immunity via Altering Gut Microbiota and Bacterial Metabolites in the Colon of Weaned Piglets.

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