Composition and Antipathogenic Activities of the Twig Essential Oil of Chamaecyparis formosensis from Taiwan

Abstract

In this study, antipathogenic activities of the twig essential oil and its constituents from Chamaecyparis formosensis Matsum were evaluated in vitro against six plant pathogenic fungi. The essential oil from the fresh twigs was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Twenty-five compounds were identified, representing 98.9% of the oil. The main components were β-eudesmol (25.1%), τ-muurolol (21.6%), elemol (15.0%), totarol (14.9%), and α-cadinol (12.4%). The twig oil (500 μg/mL) showed growth inhibitory activity against the phytopathogenic fungi, Fusarium oxysporum, Pestalotiopsis funereal, and Ganoderma austral, with antifungal indices of 92.7%, 71.1%, and 87.7%, respectively. In addition, the oil suppressed totally the growth of Rhizoctonia solani, Colletotrichum gloeosporioides, and Fusarium solani. In order to ascertain the source compounds of these antipathogenic activities, the main components were individually evaluated. τ-Muurolol and α-cadinol exhibited excellent activity against F. oxysporum, R. solani, C. gloeosporioides, and F. solani, with IC50< 50 μg/mL. These compounds also efficiently inhibited the mycelial growths of P. funereal and G. austral. Thus, α-cadinol and τ-muurolol could be considered as potential natural fungicides for controlling fungal pathogens and worth.

Effects of Essential Oil from Hinoki Cypress, Chamaecyparis obtusa, on Physiology and Behavior of Flies

“…For example, essential oil from Chamaecyparis lawsoniana has larvicidal and repellent activities against Asian tiger mosquito, Aedes albopictus [ 28 ], as well as antibacterial activities against Bacillus subtilis , Staphylococcus aureus , and Micrococcus luteus [ 29 ]. Essential oil from Chamaecypris formosensis also has growth inhibitory activity against phytopathogenic fungi [ 30 ]. In this study, we showed that ingestion and fumigation of essential oil from Chamaecyparis obtusa has insecticidal activity in fruit flies, D .…”

Effects of essential oil from Chamaecyparis obtusa on cytokine genes in the hippocampus of maternal separation rats

“…Essential oils extracted from C. obtusa and other Cupressaceae family plants contain several types of terpenes including limonene, bornyl acetate, ␣-terpineol, and elemol (Sullivan et al 1994), which are the active components that exert antigastropathic, anti-inflammatory, and antioxidant activity. Indeed, it has been shown that essential oils from Cupressaceae, including C. obtusa, have antibacterial, antifungal, and anti-cancer effects (Hong et al 2004;Li et al 2009;Ho et al 2012). In addition, a clinical study has reported that inhalation of essential oil from C. obtusa (EOCO) decreased the concentrations of stress hormones such as adrenaline and noradrenaline in humans (Li et al 2009).…”

New Terpenoids from Chamaecyparis formosensis (Cupressaceae) Leaves with Modulatory Activity on Matrix Metalloproteases 2 and 9

“…The wood is frequently used as lumber and for building, particularly temples and shrines, and for expensive furniture. The plant is known for its strong resistance against wood-decaying fungi and termites [ 7 ]. To date, only a few bioactive constituents from the roots, bark, wood, peel, and leaves from this plant have been investigated.…”

“…To date, only a few bioactive constituents from the roots, bark, wood, peel, and leaves from this plant have been investigated. [ 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ].…”