Composition and architecture of biofilms on used voice prostheses

Buijssen, Kevin J. D. A.; Laan, Bernard F. A. M. van der; Mei, Henny C. van der; Atema-Smit, Jelly; Huijssen, Pauline van den; Busscher, Henk J.; Harmsen, Hermie J. M.

doi:10.1002/hed.21833

Cited by 30 publications

(54 citation statements)

References 38 publications

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“…On most prostheses studied by Buijssen et al (2012), bacteria were mostly located near the outermost surface of the biofilm and yeasts were predominantly located near or in the silicone rubber, demonstrating in-growing of yeast colonies. It has been suggested that the adhesion of bacteria to VPs is a prerequisite for subsequent colonization by yeasts (Neu et al, 1994a).…”

Section: Microbial Composition Of Biofilms On Voice Prosthesesmentioning

confidence: 99%

“…(Neu et al, 1994b). Buijssen et al (2012) analysed 66 biofilms from several commonly used VP devices for their bacterial and fungal community population and reported the frequency with which micro-organisms were found as follows: C. albicans (77 %), C. tropicalis (52 %), Lactobacillus gasseri (96 %), Lactobacillus fermentum (73 %), Lactobacillus acidophilus (52 %) and streptococci (61 %). Lactobacilli were the predominant bacteria in voice prosthetic biofilms (Buijssen et al, 2007).…”

Section: Microbial Composition Of Biofilms On Voice Prosthesesmentioning

confidence: 99%

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Candida biofilm formation on voice prostheses

Talpaert

Balfour

Stevens

et al. 2015

Journal of Medical Microbiology

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Section: Microbial Composition Of Biofilms On Voice Prosthesesmentioning

confidence: 99%

Section: Microbial Composition Of Biofilms On Voice Prosthesesmentioning

confidence: 99%

Candida biofilm formation on voice prostheses

Talpaert

Balfour

Stevens

et al. 2015

Journal of Medical Microbiology

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“…As a consequence, these devices are found to be rapidly colonized by bacteria and yeasts, leading to deterioration of the voice prosthesis. While the functional lifetime of voice prostheses in patients, ranges from several days to years; this period is most often cut short due to rapid biofilm formation, with an average in situ lifetime of around 3 to 4 months (Van Den Hoogen et al, 1996; van Weissenbruch et al, 1997; Op de Coul et al, 2000; Buijssen et al, 2012). Biofilms formed on silicone rubber voice prosthesis, largely comprise of yeast and bacterial spp.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Profiling of C. albicans in a Two Species Biofilm with Rothia dentocariosa

Uppuluri

Busscher

Chakladar

et al. 2017

Front. Cell. Infect. Microbiol.

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Biofilms on silicone rubber voice prostheses are the major cause for frequent failure and replacement of these devices. The presence of both bacterial and yeast strains has been suggested to be crucial for the development of voice prosthetic biofilms. Polymicrobial biofilms that include Candida albicans and Rothia dentocariosa are the leading cause of voice prosthesis failure. An in vitro biofilm comprising these two organisms was developed on silicone rubber, a material used for Groningen button voice prosthesis. We found that this biofilm environment was not conducive for C. albicans growth or differentiation. Global transcriptional analyses of C. albicans biofilm cells grown with R. dentocariosa revealed that genes with functions related to cell cycle progression and hyphal development were repressed >2-fold. The mixed species biofilms were more compact and less robust compared to C. albicans mono-species biofilms, even when developed under conditions of continuous nutrient flow. Under these conditions R. dentocariosa also significantly inhibited C. albicans biofilm dispersal. Preferential adherence of R. dentocariosa to candidal hyphae was mediated by the adhesin Als3.

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“…It has been hypothesized that the interaction with bacterial strains, such as S. aureus enhances the initial steps of plaque formation and ultimate device failure. Still, to date the most commonly accepted decontamination methods are antifungal, [14, 16, 18] based on early studies conducted to characterize the effects of antifungal therapy on device life of VPs. Historical studies suggested promising results using various agents, [4, 6, 20] but the available level of evidence from early studies is low [27] and biofilms are several magnitudes more resistant to antifungal decontamination efforts than planktonic cells [28].…”

Section: Discussionmentioning

confidence: 99%

Biofilm on the tracheoesophageal voice prosthesis: considerations for oral decontamination

Somogyi-Ganss

Chambers

Lewin

et al. 2016

Eur Arch Otorhinolaryngol

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Objectives The tracheoesophageal puncture (TEP) restores verbal communication after total laryngectomy using a one-way valved voice prosthesis (VP). Microbial colonization can shorten VP device life. Our aims were to investigate patterns of prosthetic and oral colonization, and record changes in VP device life after targeted decontamination. Materials and Methods We conducted a retrospective review of TEP clinic patients who underwent microbial analysis of the VP between 01/2003 and 07/2013. Two subgroups were analyzed: 1) patients with microbial analysis of the VP and the mouth were analyzed to identify patterns of common contamination, and 2) patients who were prescribed targeted oral decontamination on the basis of the microbial analysis of the VP were analyzed to evaluate effects on device life. Results Among 42 patients, 3 patients had only fungal, 5 only bacterial, and 33 had polyspecies fungal and bacterial colonization. In the TEP-oral microflora subgroup (n=15), 7 had common microorganisms in the mouth and on the VP. Among the decontamination subgroup (n=23), 6 patients received broad spectrum rinse, 16 antifungal agents and 13 antibiotics, or a combination thereof. After targeted decontamination, the median device life of prostheses improved from 7.89 to 10.82 weeks (p=0.260). Conclusion The majority of patients with a suboptimal VP device life in this pilot had polyspecies bacterial and fungal colonization. VPs rarely had fungal contamination alone (3%), and non-albicans fungal species were more common than expected. For these reasons, we are exploring the use of targeted decontamination regimens that were associated with 1.4-fold improvement in VP duration.

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Composition and architecture of biofilms on used voice prostheses

Abstract: The results give a better understanding of biofilm formation and can be used in further development to prevent biofilm formation on voice prostheses.

Cited by 30 publications

References 38 publications

Candida biofilm formation on voice prostheses

Candida biofilm formation on voice prostheses

Transcriptional Profiling of C. albicans in a Two Species Biofilm with Rothia dentocariosa

Biofilm on the tracheoesophageal voice prosthesis: considerations for oral decontamination

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