Composition and Biological Activity of Volatile Oil from Salvia judaica and S. multicaulis from Jordan

Abstract

The aim of this work was to determine the composition of the hydro-distilled essential oil of Salvia judaica Boiss. and S. multicaulis Vahl. (Lamiaceae) from Jordan by GC and GC-MS and to report the actual composition of their fresh leaves and flowers using SPME (Solid Phase Micro-Extraction).Their dual alphaamylase/alpha glucosidase and pancreatic lipase inhibitory activities as well as their anti-proliferative potential were screened. The aroma profile of the leaves, flowers, and flowers at pre-flowering stages of S. judaica, obtained through SPME was composed of sesquiterpene hydrocarbons (87.7 %, 71.8 %, and 86.2 %, respectively) while the hydro-distilled oil of the dry leaves was rich in oxygenated sesquiterpenes (50.8%). Fresh leaves of S. multicaulis were rich in oxygenated monoterpenes (58.1%), while monoterpene hydrocarbons dominated the blooming flowers (57.2%) and the flowers at the pre-flowering stage (64.7%). The hydro-distilled oil of the dry leaves was rich in oxygenated monoterpenes (77.6%). With doxorubicin as a positive control, no anti-proliferative activity was observed against colorectal cancer cell lines HT29, HCT116, and SW620 using SRB assay for either Salvia spp. In vitro enzymatic starch digestion was evaluated with Acarbose (IC 50 : 0.2± 0.0 µg /mL) as the reference drug. The respective IC 50 (mg/mL) values of S. judaica and S. multicaulis aqueous extracts were 4.9 ± 0.4 and 10.3 ± 0.9. Modulation of pancreatic lipase activity (PL) was determined by colorimetry and compared with Orlistat (IC 50 : 0.11 ± 0.0 μg/mL). PL-IC 50 values (μg/mL) obtained for S. judaica and S. multicaulis were 108.5±6.4 and 31.8 ± 0.8, respectively.

Aroma Profile of Two Populations of Salvia verbenaca Collected from Two Bio‐Geographical Zones from Jordan

“…The actual temperature in the MS source was 180 °C and ionization voltage was 70 eV. The temperature program employed was similar to that described in the literature . Briefly, column temperature was held constant (60 °C, isothermal) for 1 min, and then ramped to 246 °C at a rate of 3 °C/min, and finally kept constant (isothermal) at 246 °C for another 3 min.…”

“…Solid Phase Micro‐Extraction (SPME) was performed according to the procedure mentioned in the literature using the same SPME‐fiber and assemblies for manual sampling (Polydimethylsiloxane/Divinylbenzene, PDMS/DVB; df 65 μm partially cross‐linked phase, fiber length 1 cm, Supelco, Bellefonte, PA, USA). Whole aerial parts of the plant were collected, and the different organs were separated immediately for analysis.…”

“…The temperature program employed was similar to that described in the literature. [4,12,17,26,27] Briefly, column temperature was held constant (60°C, isothermal) for 1 min, and then ramped to 246°C at a rate of 3°C/min, and finally kept constant (isothermal) at 246°C for another 3 min. GC/FID experiments (HewlettÀ Packard HP-8590 gas chromatograph, splitsplitless mode, split ratio 1 : 50) were performed utilizing an optima-5 fused silica gel capillary column (5 % diphenylpolysiloxane, 95 % dimethylpolysiloxane, 30 m × 0.25 mm, 0.25 μm film thickness) and using the same temperature program and chromatographic conditions performed for the GC/MS experiments.…”

“…In a continuation of our work on Salvia species from Jordan, the present work aims at reporting and comparing the chemical composition of the volatile organic compounds (VOCs) in the aroma emitted spontaneously from the different aerial organs of S. verbenaca collected from two geographical zones, the Mediterranean and Irano‐Turanian. These VOCs were extracted by Head Space Solid Phase Micro‐Extraction (HS‐SPME) method and then analyzed by GC/MS.…”

Rhaponticum acaule (L) DC essential oil: chemical composition, in vitro antioxidant and enzyme inhibition properties

“…Using the method A (preincubation of lipase with inhibitor), the RaEO exhibited promising anti-lipase activity which is comparable with that of pure THL used as a standard inhibitor against pancreatic lipase. In the same way and according to the literature, the inhibition of pancreatic lipase by the essential oil of Salvia judaica (Lamiaceae) flowers was determined colorimetrically and compared with Orlistat used as a positive control by Afifi et al [ 48 ]. The IC 50 value (0.108 mg/mL) obtained in this study was very close to that obtained with RaEO (0.2 mg/mL).…”