Composition and biological activity of essential oils from Artemisia roxburghiana Besser and Elsholtzia fruticosa Rehder cultivated in Italy

Fusani, Pietro; Ronga, Domenico; Carminati, Domenico; Mandrioli, Mauro; Manicardi, Gian Carlo; Giannì, Sergio; Tava, Aldo

doi:10.1016/j.indcrop.2022.115317

Cited by 9 publications

(4 citation statements)

References 55 publications

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“…These molecules contribute to its complex chemical profile and therapeutic activities. EF oil exhibits antiparasitic effects on Ditylenchus destructor and antibacterial properties [24][25][26][27][28][29][30]. The anti-inflammatory and antimicrobial effects have already been demonstrated [29,30]; however, their role in obesity management remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation

Shrestha,

Kim,

Shrestha

et al. 2024

J Anim Sci Technol

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Elsholtzia fruticosa (EF) is present in tropical regions throughout South Asian countries as well as the Himalayas. Although it has been used as a traditional medicine to treat digestive, respiratory, and inflammatory issues, its effect on preadipocyte differentiation is unknown. In this study, we examined the effects of a methanol extract prepared from EF on the differentiation of 3T3-L1 preadipocytes. Cell differentiation was assessed by microscopic observation and oil-red O staining. The expression of adipogenic and lipogenic genes, including PPARγ and C/EBPα , was measured by western blot analysis and quantitative real-time polymerase chain reaction (qRT–PCR), to provide insight into adipogenesis and lipogenesis mechanisms. The results indicated that EF promotes the differentiation of 3T3-L1 preadipocytes, with elevated lipid accumulation occurring in a concentration-dependent manner without apparent cytotoxicity. EF enhances the expression of adipogenic and lipogenic genes, including PPARγ , FABP4 , adiponectin , and FAS , at the mRNA and protein levels. The effect of EF was more pronounced during the early and middle stages of 3T3-L1 cell differentiation. Treatment with EF decreased C/EBP homologous protein (CHOP) mRNA and protein levels, while increasing C/EBPα and PPARγ expression. Treatment with EF resulted in the upregulation of cyclin E and CDK2 gene expression within 24 h, followed by a decrease at 48 h, demonstrating the early-stage impact of EF. A concomitant increase in cyclin-D1 levels was observed compared with untreated cells, indicating that EF modulates lipogenic and adipogenic genes through intricate mechanisms involving CHOP and cell cycle pathways. In summary, EF induces the differentiation of 3T3-L1 preadipocytes by increasing the expression of adipogenic and lipogenic genes, possibly through CHOP and cell cycle-dependent mechanisms.

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Section: Introductionmentioning

confidence: 99%

Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation

Shrestha,

Kim,

Shrestha

et al. 2024

J Anim Sci Technol

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“…The genus Artemisia is well known for its richness in bioactive substances, which have shown potential applications in numerous fields [26,27]. The EOs extracted from Artemisia species have received extensive attention for their insecticidal properties and have proven effective against a variety of insect pests [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Promising Insecticidal Properties of Essential Oils from Artemisia aragonensis Lam. and Artemisia negrei L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: In Vitro and In Silico Approaches

Chebbac,

Benziane Ouaritini,

Allali

et al. 2023

Separations

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Artemisia negrei (A. negrei) and Artemisia aragonensis (A. aragonensis) are in the family Asteraceae, which has been used in traditional medicine. The use of plant-derived insecticides has become a promising strategy to reduce the harmful effects of synthetic insecticides and overcome the bio-resistance of pest insects to insecticides. In this regard, the purpose of the current study was to determine the chemical composition and evaluate insecticidal effects of essential oils (EOs) extracted from A. negrei (EON) and A. aragonensis (EOA). Notably, all chemical constituents present in the EOs were identified through GC-MS analysis, whilst the insecticidal properties against Callosobruchus maculatus Fab. (C. maculatus) were investigated by use of in vitro an in silico approaches. The obtained results showed that both tested EOs present a significant insecticidal effect against C. maculatus, which increased significantly upon the dose used in both contact and inhalation tests. The lethal concentrations (LC50) for the inhalation test were found to be 2.1 and 2.97 μL/L, while in the contact test they were 2.08 and 2.74 μL/L of air for EON and EOA, respectively. At 5 μL/L of air, the spawn reduction rate was 88.53 % and 77.41%, while the emergence reduction rate was 94.86% and 81.22% by EON and EOA, respectively. With increasing doses of up to 20 μL/L of air, the reduction in individual emergence reached 100% by the two oils tested after 36 h of treatment. In addition, Molecular docking (MD) simulations supported the in vitro findings and indicated that certain identified components in EOA and EON exhibited stronger hydrogen bonding interactions with the target receptors. Interestingly, the prediction of ADMET properties indicates that the molecules investigated have great pharmacokinetic profiles with no side effects. Taken together, our findings suggest that EOA and EON may exert both potential contact and inhalation insecticidal actions and could be used as an alternative tool for the control of this major insect pest of stored products.

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“…Lamiaceae is one of the most important families in terms of aromatic properties, comprising about 250 genera and more than 7,000 species, which are common to the Mediterranean and subtropical regions (Bridi et al 2021;Assaf et al 2022;Fusani et al 2022). Within this family, the genus Hyptis is considered to have great economic importance, and comprises about 280 species, which after a new circumscription based on molecular phylogenies have been split into 11 genera, including the genus Eplingiella Harley & J.F.B.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of selected species of Eplingiella (Lamiaceae) using ISSR molecular markers

Silva

Oliveira

Pastore

et al. 2023

Preprint

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The present study evaluated the population structure and genetic diversity using ISSR molecular markers in 18 natural populations belonging to three species of Eplingiella (E. cuniloides, E. fruticosa and E. brightoniae), found growing naturally in the semiarid region of Northeast Brazil. Samples of 265 plants were analyzed using nine primer combinations, which generated 131 informative bands. Eplingiella spp. populations showed moderate genetic diversity (percentage of polymorphic bands, PPB = 75.6–96.9%, Nei's genetic diversity He = 0.31–0.39, Shannon's information index I = 0.33–0.48). Molecular variance analysis (AMOVA) revealed that within populations, variations contributed more (74%) to the genetic diversity than between population variations (26%), with percentage of the genetic differentiation coefficient (GST= 0.29). The mean value of FST was 0.175, demonstrating good differentiation between populations. The analysis of the structure by the Bayesian method revealed the formation of two groups (K = 2), with many migrant individuals and a high level of miscegenation. The hierarchical cluster dendrogram grouped the 18 populations into two major clusters, with good support for the main clades (100%). According to principal component analysis (PCA), the two main principal components explained 21.06% of the total variation. The ISSR molecular markers used were effective in identifying the variability of natural populations of Eplingiella spp., and population structure demonstrated recent diversification of species. The results shed more light on the genetic variation and evolutionary dynamics of Eplingiella, helping to formulate effective breeding strategies.

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Composition and biological activity of essential oils from Artemisia roxburghiana Besser and Elsholtzia fruticosa Rehder cultivated in Italy

Cited by 9 publications

References 55 publications

Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation

Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation

Promising Insecticidal Properties of Essential Oils from Artemisia aragonensis Lam. and Artemisia negrei L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: In Vitro and In Silico Approaches

Genetic diversity and population structure of selected species of Eplingiella (Lamiaceae) using ISSR molecular markers

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