Composition and diversity of the preputial microbiota in healthy bulls

Wickware, Carmen L.; Johnson, Timothy A.; Koziol, Jennifer

doi:10.1016/j.theriogenology.2019.11.002

Cited by 24 publications

(33 citation statements)

References 28 publications

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“…For example, the discovery of Campylobacter fetus subsp. venerealis in the metagenomics study investigating preputial samples from healthy bulls reinforced the previous findings regarding the role of bulls in spreading venereal diseases while remaining asymptomatic [ 17 , 18 , 20 , 60 ]. Meanwhile, metagenomics studies also revealed that the lower reproductive tract of cows has a different microbial composition compared to primates and humans, which both have Lactobacillus spp.…”

Section: Discussionsupporting

confidence: 80%

“…The close anatomical proximity between the two systems may have allowed the colonization of the reproductive tract microbial community originating from the gastrointestinal tract [ 56 , 82 , 99 ]. In addition, the possibility of direct faecal and environmental contamination of the reproductive organs should be taken into consideration [ 60 , 82 ].…”

Section: Discussionmentioning

confidence: 99%

“…Amplicon sequencing is a targeted sequencing approach focusing on a specific genomic region which are ubiquitous and discriminatory throughout the microbial population of interest [ 58 , 59 ]. The common target genes used in amplicon sequencing are 16S rRNA genes for bacteria [ 60 ], 18S rRNA genes for eukaryotes [ 61 ] and internal transcribed spacer (ITS) genes for fungi [ 62 ]. The drawback of amplicon sequencing are the biases associated with the usage of different variable regions of the target genes as the amplicon primers [ 63 ].…”

Section: Introductionmentioning

confidence: 99%

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Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

et al. 2021

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Undesirable microbial infiltration into the female bovine reproductive tracts, for example during calving or mating, is likely to disturb the commensal microflora. Persistent establishment and overgrowth of certain pathogens induce reproductive diseases, render the female bovine reproductive tract unfavourable for pregnancy or can result in transmission to the foetus, leading to death and abortion or birth abnormalities. This review of culture-independent metagenomics studies revealed that normal microflora in the female bovine reproductive tract is reasonably consistently dominated by bacteria from the phyla Bacteroidetes, Firmicutes, Proteobacteria, following by Actinobacteria, Fusobacteria and Tenericutes. Reproductive disease development in the female bovine reproductive tract was demonstrated across multiple studies to be associated with high relative abundances of bacteria from the phyla Bacteroidetes and Fusobacteria. Reduced bacterial diversity in the reproductive tract microbiome in some studies of cows diagnosed with reproductive diseases also indicated an association between dysbiosis and bovine reproductive health. Nonetheless, the bovine genital tract microbiome remains underexplored, and this is especially true for the male genital tract. Future research should focus on the functional aspects of the bovine reproductive tract microbiomes, for example their contributions to cattle fertility and susceptibility towards reproductive diseases.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

et al. 2021

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“…Dentro da cavidade prepucial existem microrganismos que são importantes para a homeostase do ambiente prepucial, no entanto desequilíbrios dessa microbiota podem causar doenças. Em situações de trauma ou em condições de imunossupressão, alguns microrganismos patogênicos podem se multiplicar e ocasionar infecções oportunistas (WICKWARE et al, 2020). Quanto maior a idade do touro, mais profundos os sulcos das criptas longitudinais na mucosa prepucial (Figura 11), e maiores são as chance de alojamento e multiplicação de agentes infecciosos de duas doenças venéreas dos bovinos: a tricomoníase genital bovina causada pelo protozoário flagelado Tritrichomonas fetus e a campilobacteriose genital bovina causada pela bactéria Campylobacter fetus venerealis (OYHENART et al, 2018;SILVEIRA et al, 2018).…”

Section: A Relação Entre Aspectos Morfológicos Do Prepúcio E Doenças Prepuciaisunclassified

Aspectos Morfológicos Da Genitália Externa De Touros Associados À Impotência Coeundi

Freitas¹,

Rabelo²,

Assis³

et al. 2021

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The bull is essential for the realization of natural breeding, which is still the majority in Brazilian livestock, and for the genetic improvement of its descendants. The bull's external genitalia are made up of the foreskin and penis and its vital function is to effect copulation. However, these organs are vulnerable and susceptible to injury due to variation in morphological characteristics, such as the width of the preputial ostium orifice, the pendulum and the length of the preputial sheath. Therefore, the aim of this study was to carry out a literature review of the morphological aspects of the external genitalia of bulls associated with coeundi impotence due to preputial diseases. The prepuce or preputial sheath consists of the external (LPE) and internal (LPI) preputial leaflets. The first is formed by a fold of mobile skin attached to the belly of the abdomen. The IPL or preputial mucosa is characterized as a tubular organ that houses the free part of the penis. The preputial ostium is the opening through which the exteriorization of the penis takes place. The foreskin has sebaceous glands that produce a lipid secretion, which makes up the smegma, and which is important in prepucial and penile health. In this study, basic research was used as an analysis tool, according to veterinary anatomical nomenclature, on aspects of the anatomy and morphology of the external genitalia, specifically the prepuce, the free part of the penis and the preputial sebaceous glands, points rarely mentioned in the specialized literature, which demonstrates the importance of this line of study.

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“…were reported to be among the main isolated micro‐organisms in camel (Ghoneim et al 2014). Despite the few reports, this microbiological approach of the reproductive tracts can lead to a better understanding of animal physiology and reproductive health (Wickware et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Composition of semen and foreskin mucosa aerobic microbiota and its impact on sperm parameters of captive collared peccaries ( Pecari tajacu)

et al. 2020

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Aim: To evaluate the bacterial composition of collared peccary semen and foreskin mucosa, and to verify the sensitivity of isolates to antimicrobials used in semen conservation and to Aloe vera gel, which is an alternative external cryoprotectant. Methods and Results: Nine foreskin mucosa and ejaculate samples from adult animals were used. Sperm characteristics and bacterial load were evaluated in fresh semen. The preputial mucosa and semen bacterial isolates were identified and tested against five concentrations of each antimicrobial (streptomycinpenicillin and gentamicin) and A. vera gel. Corynebacterium sp. and Staphylococcus sp. were isolated in greater numbers than others in both semen (64Á10 and 20Á51%, respectively) and the foreskin mucosa (60Á60 and 24Á25%, respectively), and ranged from 0Á4 to 21 9 10 5 colony-forming units (CFU) per ml. The average load of Corynebacterium sp. was negatively correlated (P < 0Á05) with the sperm membrane integrity (r = À0Á73055) and curvilinear velocity (r = À0Á69048). Streptomycin-penicillin and gentamicin inhibited most microorganisms , and A. vera showed lower antimicrobial activity. Conclusion: Several Gram-positive bacteria are present in semen and foreskin mucosa of collared peccary, and the benefits of using primarily penicillinstreptomycin and gentamicin antimicrobials in the bacterial control of diluted semen of these animals are strongly indicated. Significance and Impact of the Study: This study provides insight into the reproductive microbiota of captive male-collared peccary. This work provides a theoretical basis to assist reproductive biotechnologies for ex situ conservation of the species.

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Composition and diversity of the preputial microbiota in healthy bulls

Cited by 24 publications

References 28 publications

Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

Aspectos Morfológicos Da Genitália Externa De Touros Associados À Impotência Coeundi

Composition of semen and foreskin mucosa aerobic microbiota and its impact on sperm parameters of captive collared peccaries ( Pecari tajacu)

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