Composition, antioxidant and antimicrobial activity of the essential oils of a full grown tree of Pinus cembra L. from the Calimani mountains (Romania)

Abstract

This study was carried out to investigate the chemical composition, the antioxidant and antimicrobial effects of the essential oils of Pinus cembra L. needles and twigs. The chemical composition was analyzed using both GC and GC-MS techniques. α-Pinene (69.14%) was the major constituent of the needle essential oil while the twig essential oil was characterized by a high content of limonene+ β-phellandrene (40.97%) and α-pinene (24.94%). The needle and twig essential oils showed weak DPPH radical scavenging effects (EC50=19.93±0.75 and EC50=18.66±0.70 mg ml-1, respectively). In antimicrobial assays, both essential oils showed high activity against Sarcina lutea and Staphylococcus aureus and no activity against Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. The needle and twig essential oils had similar antimicrobial effects against Sarcina lutea with MIC and MBC values of 0.12 and 0.24 mg ml-1, respectively. The twig essential oil (MIC=1.95 mg ml-1, MBC=3.9 mg ml-1) was more active against Staphylococcus aureus than the needle essential oil (MIC=3.9 mg ml-1, MBC=15.62 mg ml-1) and also exhibited a moderate activity against Candida albicans (MIC=7.81 mg ml-1, MFC=15.62 mg ml-1)

Coriander essential oil and linalool - interactions with antibiotics against Gram-positive and Gram-negative bacteria

“…; Lungu Apetrei et al . ). The compounds were identified by comparison of their mass spectral data with those of National Institute of Standards and Technology (NIST) library data.…”

“…The components concentrations were obtained from the FID peak areas without using correction factors (Lungu Apetrei et al . ).…”

Synthesis and In vitro Analysis of Novel Dihydroxyacetophenone Derivatives with Antimicrobial and Antitumor Activities

“…The most active compounds were subjected to a broth micro dilution assay in order to determinate the minimum inhibitory concentrations (MIC) and the minimum bactericidal/fungicidal concentrations (MBC/MFC) [27][28][29] ( Table 3). It is worthy to note that all the brominated compounds showed good antibacterial effects.…”

“…A broth microdilution assay was used to determine the minimum inhibitory concentrations (MIC) and the minimum bactericidal/fungicidal concentrations (MBC/MFC) [27][28][29]. The compounds were dissolved in dimethyl sulfoxide at a concentration of 10 mg/mL.…”

“…The plates were incubated at 37 °C for antibacterial tests and 24 °C for antifungal tests, for 24 h. The MIC value represents the lowest concentration of compound inhibiting the visible growth of microorganisms. The MBC/MFC values represent the lowest concentration of the compound killing completely the microorganisms to be tested, were determined by transferring 10 μL of samples showing inhibition of visible growth on the surface of an agar plate [28]. The subcultures were incubated at 37 °C for antibacterial tests and 24 °C for antifungal tests for 24 h. There were also evaluated the MIC and MBC/MFC values of ampicillin/nystatin towards bacteria/yeast strains.…”