Composition Diversity and Abundance of Gut Microbiome in Prediabetes and Type 2 Diabetes

Lambeth, Stacey M; Carson, Trechelle; Lowe, Janae; Ramaraj, Thiruvarangan; Leff, Jonathan W.; Luo, Lei; Bell, Callum J.; Shah, Vallabh O.

doi:10.15436/2376-0949.15.031

Cited by 174 publications

(97 citation statements)

References 38 publications

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“…First, α-diversity was higher in cases. This finding contrasts with few early reports in T2D where α-diversity is often decreased (Larsen et al, 2010;Lambeth et al, 2015;Dominguez-Bello et al, 2019). This observation may be due to treatment and/or lifestyle changes (e.g., diet, physical activity) that are prescribed following diabetes diagnosis as supported by our analyses that treatment may contribute to increased α-diversity.…”

Section: Discussioncontrasting

confidence: 98%

Gut Microbiome Profiles Are Associated With Type 2 Diabetes in Urban Africans

Doumatey

Adeyemo

Zhou

et al. 2020

Front. Cell. Infect. Microbiol.

108

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Gut dysbiosis has been associated with several disease outcomes including diabetes in human populations. Currently, there are no studies of the gut microbiome composition in relation to type 2 diabetes (T2D) in Africans. Here, we describe the profile of the gut microbiome in non-diabetic adults (controls) and investigate the association between gut microbiota and T2D in urban West Africans. Gut microbiota composition was determined in 291 Nigerians (98 cases, 193 controls) using fecal 16S V4 rRNA gene sequencing done on the Illumina MiSeq platform. Data analysis of operational taxonomic units (OTU) was conducted to describe microbiome composition and identify differences between T2D and controls. The most abundant phyla were Firmicutes, Actinobacteria, and Bacteroidetes. Clostridiaceae, and Peptostreptococcaceaea were significantly lower in cases than controls (p < 0.001). Feature selection analysis identified a panel of 18 OTUs enriched in cases that included Desulfovibrio piger, Prevotella, Peptostreptococcus, and Eubacterium. A panel of 17 OTUs that was enriched in the controls included Collinsella, Ruminococcus lactaris, Anaerostipes, and Clostridium. OTUs with strain-level annotation showing the largest fold-change included Cellulosilyticum ruminicola (log 2 FC = −3.1; p = 4.2 × 10 −5), Clostridium paraputrificum (log 2 FC = −2.5; p = 0.005), and Clostridium butyricum (log 2 FC = −1.76; p = 0.01), all lower in cases. These findings are notable because supplementation with Clostridium butyricum and Desulfovibrio piger has been shown to improve hyperglycemia and reduce insulin resistance in murine models. This first investigation of gut microbiome and diabetes in urban Africans shows that T2D is associated with compositional changes in gut microbiota highlighting the possibility of developing strategies to improve glucose control by modifying bacterial composition in the gut.

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Section: Discussioncontrasting

confidence: 98%

Gut Microbiome Profiles Are Associated With Type 2 Diabetes in Urban Africans

Doumatey

Adeyemo

Zhou

et al. 2020

Front. Cell. Infect. Microbiol.

108

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“…Similarly, previous studies examining the relation of Shannon Index with CVD risk factors have generally showed null results. 33, 34 These data suggest that the abundance of distinct microbial taxa may be more important than their relative frequency in determining CVD risk.…”

Section: Discussionmentioning

confidence: 90%

Gut Microbiome Associates With Lifetime Cardiovascular Disease Risk Profile Among Bogalusa Heart Study Participants

Kelly

Bazzano

Ajami

et al. 2016

Circulation Research

290

214

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Rationale Few studies have systematically assessed the influence of gut microbiota on cardiovascular disease (CVD) risk. Objective To examine the association between gut microbiota and lifetime CVD risk profile among 55 Bogalusa Heart Study (BHS) participants with the highest and 57 with the lowest lifetime burdens of CVD risk factors. Methods and Results 16S rRNA sequencing was conducted on microbial DNA extracted from stool samples of the BHS participants. Alpha diversity, including measures of richness and evenness, and individual genera were tested for associations with lifetime CVD risk profile. Multivariable regression techniques were employed to adjust for age, gender, and race (Model 1), along with body mass index (BMI) (Model 2) and both BMI and diet (Model 3). In Model 1, odds ratios (95% confidence intervals) for each standard deviation increase in richness, measured by the number of observed operational taxonomic units, Chao 1 index, and abundance-based coverage estimator, were 0.62 (0.39, 0.99), 0.61 (0.38, 0.98), and 0.63 (0.39, 0.99), respectively. Associations were consistent in Models 2 and 3. Four genera were enriched among those with high versus low CVD risk profile in all models. Model 1 p-values were: 2.12×10−3, 7.95×10−5, 4.39×10−4, and 1.51×10−4 for Prevotella 2, Prevotella 7, Tyzzerella and Tyzzerella 4, respectively. Two genera were depleted among those with high versus low CVD risk profile in all models. Model 1 P-values were: 2.96×10−6 and 1.82×10−4 for Alloprevotella and Catenibacterium, respectively. Conclusions The current study identified associations of overall microbial richness and six microbial genera with lifetime CVD risk.

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“…The abundance of the genus Roseburia was negatively correlated with the plasma glucose levels in a human study [56], and the depletion of the genus Roseburia is a characteristic feature of the microbiome in type 2 diabetic patients [57]. In addition, a high abundance of the genus Collinsella is a characteristic feature of the microbiome in type 2 diabetic patients [58], and a structured weight loss program reduced the abundance of the genus Collinsella in obese type 2 diabetic patients [59]. Based on these insights, the alterations in the abundances of Roseburia and Collinsella observed in our study could have affected obesity in these patients.…”

Section: Discussionmentioning

confidence: 98%

Daily Intake of Paraprobiotic Lactobacillus amylovorus CP1563 Improves Pre-Obese Conditions and Affects the Gut Microbial Community in Healthy Pre-Obese Subjects: A Double-Blind, Randomized, Placebo-Controlled Study

Sugawara¹,

Sawada²,

Yanagihara³

et al. 2020

Microorganisms

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Despite the fact that gut microbiota is closely associated with obesity, few studies have focused on the influences of paraprobiotics as food ingredients on both obesity prevention and the gut microbial community. In this study, we evaluated the effects of fragmented Lactobacillus amylovorus CP1563 (CP1563) as a paraprobiotic for obesity prevention and investigated its effects on the gut microbial community in pre-obese subjects. One hundred sixty-nine healthy subjects with a body mass index from 25.0 to 29.9 kg/m2 ingested beverages with or without the fragmented CP1563 containing 10-hydroxyoctadecanoic acid (10-HOA) for 12 weeks. The changes in abdominal, total, visceral, and subcutaneous fatty areas were significantly lower in the CP1563-10-HOA group than in the placebo group at 12 weeks. Furthermore, 16S rRNA gene sequencing of fecal DNA revealed that the changes in the abundances of the genera Roseburia and Lachnospiraceae;g were significantly greater in the CP1563-10-HOA group than in the placebo group, and the changes in the abundances of the genus Collinsella was significantly smaller in the CP1563-10HOA group than in the placebo group. Our results showed that continuous ingestion of the fragmented CP1563 containing 10-HOA reduced abdominal body fat and affected the gut microbial community in pre-obese healthy subjects. Our findings may contribute to the understanding of the relationship between the anti-obesity effect of paraprobiotics and gut microbiota.

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Composition Diversity and Abundance of Gut Microbiome in Prediabetes and Type 2 Diabetes

Cited by 174 publications

References 38 publications

Gut Microbiome Profiles Are Associated With Type 2 Diabetes in Urban Africans

Gut Microbiome Profiles Are Associated With Type 2 Diabetes in Urban Africans

Gut Microbiome Associates With Lifetime Cardiovascular Disease Risk Profile Among Bogalusa Heart Study Participants

Daily Intake of Paraprobiotic Lactobacillus amylovorus CP1563 Improves Pre-Obese Conditions and Affects the Gut Microbial Community in Healthy Pre-Obese Subjects: A Double-Blind, Randomized, Placebo-Controlled Study

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