Composition of Colon Microbiota in Rats Treated with ACTH(4-7)-PGP Peptide (Semax) under Conditions of Restraint Stress

Svishcheva, M. V.; Mukhina, A. Yu.; Медведева, О. А.; Shevchenko, A. V.; Bobyntsev, I. I.; Калуцкий, П. В.; Andreeva, L. A.; Myasoedov, N. F.

doi:10.1007/s10517-020-04886-7

Cited by 6 publications

(4 citation statements)

References 7 publications

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“…This finding is in line with the reduced bifidobacteria load found in the colon and feces of rats and mice that underwent chronic restraint stress. 9,11 In addition, the apparent bifidobacteria amount decrease in the proximal region may be due to the bacterial load being below the limit of detection, as this area is scarcely populated with microbiota compared to the distal segment. 3 Similar to restraint and crowding stress, the stress induced in the current study promoted the loss of body weight and reduced caloric intake.…”

Section: Discussionmentioning

confidence: 99%

“…6 Experimental assays demonstrate a wide array of interweaving pathways through which stress regulates commensal microbiota 7 and TJPs. 8 Chronic stress had a downmodulatory effect on the commensal bifidobacterial density in the colon of rats under restraint stress for 2 h a day over 4 days, 9 while the abundance in metagenomic terms of Bifidobacterium pseudolongum increased in the colon of mice subjected to chronic unpredictable mild stress. 10 Moreover, the interactions between chronic stress and microbiota through the brain-gut-microbiota axis impact behavior and TJP remodeling through proinflammatory cytokines.…”

Section: Introductionmentioning

confidence: 99%

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Regionalized analysis of gut barrier components in the small intestine of BALB/c mice following chronic immobilization stress

Guzmán-Mejía¹,

Godínez‐Victoria²,

García-Hernández³

et al. 2023

Adv Clin Exp Med

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regionalized analysis of gut barrier components in the small intestine of BALB/c mice following chronic immobilization stress

Guzmán-Mejía¹,

Godínez‐Victoria²,

García-Hernández³

et al. 2023

Adv Clin Exp Med

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“…При этом изменение качественного и количественного состава микробиоты может наблюдаться даже после кратковременного стрессорного воздействия [102]. Ранее нами было показано, что хронический иммобилизационный стресс приводил к изменению состава микробиоты толстой кишки в виде уменьшения количества облигатных микроорганизмов и увеличения условно-патогенных [103][104][105][106], которое было ассоциировано с воспалительными изменениями в стенке толстой кишки [107][108][109]. При этом ежедневное внутрибрюшинное введение тафтцина-ПГП и N-синтетического аналога АКТГ, АКТГ4-7-ПГП корригировало стресс-индуцированные изменения как в микробиоте толстой кишки, так и в ее стенке [104,108,109].…”

Section: влияние метаболических факторов микробиоты на морфофункцио-н...unclassified

On mechanisms of increased intestinal wall permeability under stress

Vorvul¹,

Bobyntsev²,

Медведева³

2022

Humans and their Health

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One of the manifestations of the stress reaction in the colon is an increase in the permeability of its wall due to the development of an inflammatory reaction against the background of changes in neuroendocrine regulation and microbiota state. These processes are accompanied by significant changes in humoral homeostasis and cell activity involved in the development of an inflammatory response in the colon wall. We performed a literature review to analyze and summarize the available research data on the mechanisms of stress-induced changes in the intestine at the molecular, cellular and tissue levels involving the regulatory systems of the body. Scientific information was searched in Web Of Science, Scopus, ScienceDirect, Medline, Russian Science Citation Index (RSCI), as well as in the search engines Google Scholar, PubMed, Semantic Scholar, Taylor & Francis, Wiley Online Library and Bielefeld Academic Search Engine (BASE). Wall permeability has been shown to have a rather complex regulation involving corticotropin-releasing factor, mast cells, dendritic cells, eosinophils, macrophages, Substance P, nerve growth factor, neurotensin, microbiota metabolic factors (serotonin, short-chain fatty acids, indole derivatives and conjugated fatty acids), epigenetic mechanisms, the HES1 (Hairy/Enhancer of Split-1) - GR (glucocorticoid receptor), and the stress-associated polarity signaling pathway. Under stress, there is a change in the functioning of these mechanisms, leading to an increase in the permeability of the intestinal wall. It results in translocation of bacteria from the lumen into the underlying layers which causes activation of the immune response with subsequent development of an inflammatory reaction. The presented data testify to the prospects and validity of the development of methods of correction of stress-induced shifts in the colon by influencing the central and local mechanisms of realization of the stress response and the state of the microbiota.

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“…Влияние пептида АКТГ 6-9 -Pro-Gly-Pro на морфофункциональное Бюллетень сибирской медицины. 2023; 22 (2): 14-20 ряд его эффектов в условиях хронического иммобилизационного стресса (ХИС) [3][4][5], в том числе на состояние нервной системы в соответствии с представлениями о функциональных взаимосвязях в оси кишечник -мозг [6]. Структурно и функционально родственным семаксу пептидом является АКТГ 6-9 -Pro-Gly-Pro.…”

Section: Introductionunclassified

Effects of the ACTH<sup>6-9</sup>-Pro-Gly-Pro peptide on the morphofunctional state of rat colon under conditions of chronic restraint stress

et al. 2023

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Aim. To study the morphofunctional state of the colonic wall in rats when using the N–terminal analog of the adrenocorticotropic hormone (ACTH) ACTH6-9-Pro-Gly-Pro (ACTH6-9-PGP) peptide under chronic stress.Materials and methods. The study was performed on 55 male Wistar rats, which were divided into 5 groups (n = 11): group 1 – control group (administration of saline solution without stress); group 2 – chronic restraint stress (CRS) + administration of saline solution; group 3 – CRS + administration of ACTH6-9-PGP at a dose of 5 μg / kg; group 4 – administration of ACTH6-9-PGP at a dose of 50 μg / kg; group 5 – administration of ACTH6-9- PGP at a dose of 500 μg / kg. A histologic examination of the rat colon was performed. The histologic architecture of the colonic wall, the depth of crypts, and the number of goblet cells were assessed. Furthermore, the number of granulocytes, plasma cells, lymphocytes, macrophages, and mast cells was counted.Results. The study demonstrated that chronic (14 days) restraint stress resulted in the development of inflammations in the colonic wall of the animals. Intraperitoneal administration of ACTH6-9-PGP at doses of 50 and 500 μg / kg daily throughout the entire time of stress exposure prevented the development of stress-induced alterations observed in the control animals. At the same time, anti-inflammatory effects of the peptide in the colonic wall and a decrease in the level of corticosterone in the blood serum were noted.Conclusion. The results of this work and data from other studies on the effects of N-terminal analogs of ACTH indicate the need for studying the mechanisms of their effect on inflammation and searching for targets of ACTH6-9-PGP.

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Composition of Colon Microbiota in Rats Treated with ACTH(4-7)-PGP Peptide (Semax) under Conditions of Restraint Stress

Cited by 6 publications

References 7 publications

Regionalized analysis of gut barrier components in the small intestine of BALB/c mice following chronic immobilization stress

Regionalized analysis of gut barrier components in the small intestine of BALB/c mice following chronic immobilization stress

On mechanisms of increased intestinal wall permeability under stress

Effects of the ACTH<sup>6-9</sup>-Pro-Gly-Pro peptide on the morphofunctional state of rat colon under conditions of chronic restraint stress

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