The egg discolouration effects of including a screw-press cottonseed meal (CSM), containing 68.2 g residual lipid/kg, 290 mg cyclopropenoid fatty acids (CPFA)/kg and 1226 mg free gossypol/kg, in laying hen diets at 300 g/kg were examined. 2. Discolouration prevention methods included treating the meal with ferrous sulphate heptahydrate (FSH), in solution or as crystals, at a 4:1 weight ratio of iron to free gossypol (experiment 1), and reducing its residual lipid (CSL) content to 4.2 g/kg by hexane extraction (experiment 2). 3. In freshly laid eggs, no discolouration was observed when hens were fed a CSM-based diet containing 250 mg free gossypol/kg and 87 mg CPFA/kg in experiment 1, but slightly brown yolks were produced in experiment 2. 4. Storage of the eggs at 22°C led to yolk mottling, an effect believed to be the initial stages of the brown yolk discolouration. 5. Storage of the eggs at 5°C resulted in enhancement of the brown yolk discolouration, apricot discolouration on surfaces of most yolks and pink albumen discolouration. These effects were prevented when the CSM was extracted with hexane. Apricot yolk and pink albumen discolourations were also produced when hens were fed a non-CSM diet containing crude CSL at 20.5 g/kg. 6. Dietary CSL increased egg fat saturation, altering the ratios of stearic to oleic, palmitic to palmitoleic and heptadecanoic to heptadecenoic fatty acids. 7. Treatment of CSM with FSH reduced the slight brown yolk discolouration in fresh eggs (experiment 2) and the yolk mottling in warm-stored eggs. Both in solution and as crystals, FSH prevented the enhancement of brown yolk discolouration in cold-stored eggs, leaving most eggs with apricot yolk and pink albumen discolourations. 8. Treatment of CSM and CSL with FSH reduced the CPFA-related 107 Downloaded by [UQ Library] at 19:24 03 February 2015 108 S . PANIGRAHI AND T. W. HAMMONDS effects on yolk fat saturation, and the colour and pH changes in coldstored eggs. 9. Exposure of yolks to ammonia vapour provided a useful test to predict the development of the gossypol-related brown discolouration in stored eggs.