Composition of HMW and LMW Glutenin Subunits and Their Effects on Dough Properties, Pan Bread, and Noodle Quality of Chinese Bread Wheats

Abstract

Knowledge of composition of high molecular weight glutenin subunits (HMW‐GS) and low molecular weight glutenin subunits (LMW‐GS) and their associations with pan bread and noodle quality will contribute to genetically improving processing quality of Chinese bread wheats. Two trials including a total of 158 winter and facultative cultivars and advanced lines were conducted to detect the allelic variation at Glu‐1 and Glu‐3 loci by SDS‐PAGE electrophoresis and to understand their effects on dough properties, pan bread, and dry white Chinese noodle (DWCN) quality. Results indicate that subunits/alleles 1 and null at Glu‐A1, 7+8 and 7+9 at Glu‐B1, 2+12 and 5+10 at Glu‐D1, alleles a and d at Glu‐A3, and alleles j and d at Glu‐B3 predominate in Chinese germplasm, and that 34.9% of the tested genotypes carry the 1B/1R translocation (allelic variation at Glu‐D3 was not determined because no significant effects were reported previously). Both variations at HMW‐GS and LMW‐GS/alleles and loci interactions contribute to dough properties and processing quality. For dough strength related traits such as farinograph stability and extensigraph maximum resistance and loaf volume, subunits/alleles 1, 7+8, 5+10, and Glu‐A3d are significantly better than those of their counterpart allelic variation, however, no significant difference was observed for the effects of d, b, and f at Glu‐B3 on these traits. For extensigraph extensibility, only subunits 1 and 7+8 are significantly better than their counterpart alleles, and alleles d and b at Glu‐B3 are slightly better than others. For DWCN quality, no significant difference is observed for HMW‐GS at Glu‐1, and Glu‐A3d and Glu‐B3d are slightly better than other alleles. Glu‐B3j, associated the 1B/1R translocation, has a strong negative effect on all quality traits except protein content. It is recommended that selection for subunits/alleles 1, 7+8, 5+10, and Glu‐A3d could contribute to improving gluten quality and pan bread quality. Reducing the frequency of the 1B/1R translocation will be crucial to wheat quality improvement in China.

Allelic diversity of HMW and LMW glutenins in Indian wheats and their relationship with sedimentation volume and mixograph parameters

“…There was significant effect of Glu-B1 and Glu-D1 loci on SDS-sedimentation volume as reported by several other workers (Payne 1987;He et al 2005;Liu et al 2005). However, Hernández et al (2012) showed no significant effect of Glu-1 loci on SDS-sedimentation.…”

Effect of high and low molecular weight glutenin subunits, and subunits of gliadin on physicochemical parameters of different wheat genotypes

“…The alleles 1 and 2* of Glu-A1 have been found to have a better effect on bread-making quality when compared to a null allele (GUPTA et al, 1994;HE et al, 2005). The 5 + 10 alleles of the Glu-D1 are correlated with higher dough strength, whereas the 2 + 12 alleles are correlated with low bread-making quality (BRANLARD; DARDEVET, 1985;GIANIBELLI et al, 2001).…”

“…He et al (2005) studied the effects of the subunits of each locus on gluten quality for bread-making from Chinese wheat. These authors found that the combinations 1 of Glu-A1, 7 + 8 of Glu-B1, 5 + 10 of Glu-D1, and d of Glu-A3 could contribute to an improved quality of wheat genotypes.…”

Effect of allelic variation in three glutenin loci on dough properties and bread-making qualities of winter wheat

“…The HMW-GS pair 5 + 10 encoded by Glu-D1d on the 1D chromosome contributes to strong dough and good bread-making qualities , Cressy et al 1987, Lagudah et al 1987, Payne et al 1981. HMW-GS 1 and 2* encoded by Glu-A1a and Glu-A1b, respectively, on the 1A chromosome and 7 + 8, 7 + 9 and 17 + 18 encoded by Glu-B1b, Glu-B1c and Glu-B1i, respectively, on the 1B chromosome also contribute to strong dough and good bread-making qualities , Cressy et al 1987, He et al 2005, Lagudah et al 1987, Lawrence et al 1984, Moonen et al 1982, Wrigley 2003.…”