Composition of main haematopoietic compartments in normal and bled channel catfish

Abstract

Differential cell counts showed that the head and trunk kidney of control and bled channel catfish Ictalurus punctatus had myeloid characteristics. They contained lymphoid and granuloid cells, thrombocytes, erythroid and agranular cells in decreasing order of abundance (%). Among the blast and precursor cells, the most numerous erythroid ones were followed by granuloid, lymphoid and agranular ones. The main changes after blood withdrawal were the decrease of thrombocytes and the increase of precursor cells in both kidney parts. In the group examined 7 days after bleeding the head kidney had a higher percentage of erythroid cells and lymphocyte precursors than the trunk kidney while the latter had more granuloid cells and their precursors. Basophils were present (c. 1%) in both regions of the kidney of all groups. The spleen was predominantly a lymphatic organ. It contained c. 80% lymphoid cells, a higher incidence of granulated lymphocytes than in kidneys, 15% thrombocytes and 1·4% agranular cells. Blood withdrawal caused an increase of thrombocytes, a decrease of lymphoid cells and an increase of erythroid precursors in the spleen. The last probably stemmed from the circulation. While haematocrit values failed to indicate the anaemic state in the bled groups, the differential red blood cell count showed dramatic differences between the control and bled groups as well as between the two groups in different stages of recuperation from the blood loss.

Haematopoiesis in the head kidney of common carp (Cyprinus carpio L.): a morphological study

“…It was similar to the values reported by Wlasow and Dabrowska (1989) in common carp (2.54%) and by Fijan (2002b) in channel catfish (2.84%). Blast cells (Fig.…”

“…In this study, the polychromatic erythroblast was the most frequent erythroid cell (8.28 ± 0.95%), similarly as observed by Wlasow and Dabrowska (1989): 15.9 ± 6.43% and Fijan (2002b): 4.61 ± 3.18%. The cytoplasm of the polychromatic erythroblast ( Fig.…”

“…In the present study, the percentage of erythroid lineage cells (13.14 ± 1.00%) was similar to the results obtained by Fijan (2002b): 13.0 ± 5.1% for channel catfish and considerably lower in relation to the results obtained by Peters and Schwarzer (1985), near 45% for rainbow trout and Wlasow and Dabrowska (1989) near 37% for common carp. Number of observed erythroid developmental stages was higher than numbers of developmental stages of any other lineage.…”

“…2.7) was the most frequent granuloid cell (8.40 ± 0.97%), similarly as noted by Wlasow and Dabrowska (1989): 3.40 ± 1.62%, and by Fijan (2002b): 4.53 ± 1.53% (who counted together neutrophilic progranulocyte and neutrophilic metagranulocyte). It showed light bluish cytoplasm, a round and mostly eccentric nucleus which occupied about half of the cell.…”

Morphological studies of peripheral blood cells of the Chinese sturgeon, Acipenser sinensis

“…Chinese sturgeon blood contained a larger number of mature erythrocytes than immature erythrocytes, in direct proportion to their stage of maturity; these proportions can therefore be used as erythropoietic activity change indicators (Fijan 2002b). Because erythropoiesis in fishes can be affected by anemia, temperature, seasonality (Lecklin and Nikinmaa 1998), and bleeding (Fijan 2002b), this could affect the number of circulating reticulocytes, which occupied almost 11.31% of whole erythrocytes in our study. The reticulocyte count may be an indicator of a regenerative response that can be modulated by anemic processes and environmental factors, for example temperature and oxygen availability.…”