Composition of Rumen Bacterial Community in Dairy Cows With Different Levels of Somatic Cell Counts

Zhong, Yifan; Xue, Mingyuan; Liu, Jianxin

doi:10.3389/fmicb.2018.03217

Cited by 53 publications

(66 citation statements)

References 41 publications

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“…However, the content of total milk protein in CM group were the highest among the 3 groups. Generally, milk yield is inversely proportional to milk protein [26]. In line with report by Rhone et al [3], decreased milk yield resulted in an increase of dry matter concentration and the total protein rate in milk.…”

Section: Discussionsupporting

confidence: 76%

“…Shifts in microbial populations associated with dysbiosis in rumen cause translocation and systemic dissemination of increased concentrations of potentially toxic and in ammatory substances, such as lipopolysaccharide [34]. In the present study, the main phyla of rumen microorganisms, Firmicutes, Proteobacteria and Bacteroidetes, in cows with H, SM and CM were not signi cantly differed, which suggested that the bacterial community with crucial function in rumen was relatively stable in cows under different udder health status [26]. Subsequent microbial HCA and PCoA analysis veri ed that only partial differences existed in rumen microbes from H and SM cows, which consisted with the results of Zhong et al [26].…”

Section: Discussionmentioning

confidence: 41%

“…Screening out cows with H, SM and CM through comprehensive judgement improved the prediction accuracy of disease incidence and progression [24,25]. Besides, unlike the work of Zhong et al [26], we increased the difference of SCC levels among the 3 groups and considered udder clinical symptoms when grouping to further investigate the correlation between BM and changes in rumen micro ora and metabolites.…”

Section: Discussionmentioning

confidence: 99%

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Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis

Wang

Nan²,

Zhao³

et al. 2020

Preprint

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Background: With high prevalence and complex etiology, bovine mastitis (BM) often causes serious damage to dairy cows’ health and milk quality. Although the shift in milk has been the main research interest on mastitis, recent studies have suggested that gastrointestinal microorganism has a crucial effect on the inflammation of the tissues outside the gut, such as mammary gland. However, studies related to the variation of rumen inner-environment during mastitis were limited. This study compared the differences of rumen microorganisms and metabolites under different udder health conditions, in order to investigate the correlation between udder health status and rumen microbiome structure and metabolites activity. Based on the somatic cell counts (SCC), California mastitis test (CMT) parameters and clinical symptoms of mastitis, 60 lactating Holstein dairy cows with similar body conditions (excepted for udder health condition) were divided into 3 groups (n = 20 per group): healthy (H) group, subclinical mastitis (SM) group and clinical mastitis (CM) group. Lactation and rumen fermentation parameters were detected, and rumen microbiota and metabolites were analyzed via 16S rRNA amplicon sequencing and untargeted metabolomics, respectively. Results: As the degree of mastitis increased, milk SCC and milk protein were significantly increased (P < 0.01). Milk yield, milk fat and lactose (P < 0.01), and rumen lactic acid (LA) (P < 0.01), acetate, propionate, butyrate, valerate (P < 0.001), total volatile fatty acids (TVFAs) concentrations (P < 0.01) and pH value (P = 0.024) were significantly decreased. In the rumen of CM cows, the significantly increased pathogens related to intestinal and oral inflammation, such as g__Lachnospiraceae_UCG-006 (P = 0.03), g__Moraxella and g__norank_f__Neisseriaceae (P < 0.01), etc., were accompanied by a significant increase in 12-oxo-20-dihydroxy-leukotriene B4 and 10beta-Hydroxy-6beta-isobutyrylfuranoeremophilane (P < 0.01). Meanwhile, in the rumen of SM cows, the g__Ruminiclostridium_9 (P = 0.04) and g_Enterorhabdus (P = 0.01) were increased with methenamine, 5-Hydroxymethyl-2-furancarboxaldehyde (5-HMF) and 6-Methoxymellein (P < 0.01). During mastitis, the decrease of short-chain fatty acids (SCFAs)-producing bacteria and intestinal probiotics in rumen, including g__Prevoterotoella_1 and g__Bifidobacterium (P = 0.03), etc., were significantly reduced, accompanied by a reduction in 2-Phenylbutyric acid (2-PBA) (P < 0.01).Conclusion: This study suggested that the microflora and metabolites associated with pathogen invasion, inflammatory response and rumen wall damage in the rumen of cows suffered mastitis were prominent.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 41%

Section: Discussionmentioning

confidence: 99%

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Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis

Wang

Nan²,

Zhao³

et al. 2020

Preprint

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“…At the genus level, Bacteroidales and Escherichia-Shigella tended to decrease in the treatment groups.Bacteroides, as a normal gut microbiota in the gut of ruminants, can cause an endogenous infection if the immune system or intestinal microbiota are dysfunctional Wang et al (2019). Succinivibrio is one of the main saccharolytic bacteria(Zhong, Xue, & Liu, 2018) Joch, Mrazek, Skrivanova, Cermak, and Marounek (2018). Likewise, Escherichia-Shigella is related to diarrhoea(Sousa, Mendes, Apolônio, Farias, & Magalhaes, 2010).…”

mentioning

confidence: 99%

Positive effects of dietary supplementation of three probiotics on milk yield, milk composition and intestinal flora in Sannan dairy goats varied in kind of probiotics

Cheng

Wang

et al. 2019

Animal Physiology Nutrition

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In this study, we investigated the effects of Saccharomyces cerevisiae (SC), Bacillus subtilis (BS) and Enterococcus faecalis (EF), singly and in combination, on the dry matter intake (DMI), milk production and composition, and faecal microflora of Saanen dairy goats. Fifty goats were randomly divided into five groups: (a) basal diet (control);(b) basal diet + SC; (c) basal diet + BS; (d) basal diet + EF; and (e) basal diet + mixed probiotics. Each treated animal received 5 g/d of probiotics for a total administration of 5 × 1,011 CFU/goat per day. The inclusion of B. subtilis and E. faecalis in the diet of lactating Saanen goats increased DMI (p < .05). Enhanced milk yield was observed with BS and EF. Milk fat percentage was significantly increased by feeding mixed probiotics compared with the control (p < .05); supplying SC, BS and mixed probiotics enhanced the protein percentage (p < .05). The milk lactose percentage in the SC and BS groups was higher than in the control (p < .05). The amount of milk total solids was higher after feeding EF or mixed probiotics than in the control group (p < .05). Non-fat solids showed no notable differences among groups (p > .05). There was no significant influence on gut bacterial abundance and diversity from adding these three probiotics, singly or in combination. Bacteroidales, Escherichia-Shigella and Christensenellaceae abundances were decreased by supplying these probiotics but Succinivibrionaceae increased. In conclusion, there were positive influences of probiotic feed supplementation on intake, milk performance and intestinal microecology. K E Y W O R D SBacillus subtilis, DMI, Enterococcus faecalis, intestinal microecology, Saccharomyces cerevisiae

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“…Mrázek et al [21] reported that high-ber intake essentially increases Butyrivibrio in rumens, whereas high-energy food additives suppress it. This genus also signi cantly differed among somatic cell count groups [22].…”

Section: Discussionmentioning

confidence: 94%

The Rumen Microbiome in Dry Dairy Cows Reveals the Specific Bacteriome Contributing to Various Physiological Roles and Phenotypes

Chuang¹,

Ho²,

Tu³

et al. 2020

Preprint

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Background Most microbiome studies of dairy cows have investigated the compositions and functions of rumen microbial communities in lactating dairy cows. The importance of the relationships among hosts, microbiota, diet composition, and milk production remains unknown in dry dairy cows. Thus, in the present study, the composition of the rumen microbiome in cows from three dairy farms was investigated to identify core bacteria contributing to various physiological roles during rumen fermentation in dry dairy cows. Results The results indicated that ruminal fluid in dry dairy cows from different regional farms had core rumen microbiota that could be clearly distinguished from that of cows of the other farms. Further identification of key microorganisms associated with each farm revealed that Prevotella, Methanobrevibacter, Pseudobutyrivibrio, Ruminococcus, Bacteroides, and Streptococcus were major contributors. Spearmen’s correlation indicated that the abundance of genera such as Prevotella and Ruminococcus in dry dairy cows could indicate milk yield in the previous lactating period. Functional pathway analysis of the rumen bacterial communities demonstrated that amino acid metabolism and carbohydrate metabolism were the major pathways. Conclusions Our findings provide knowledge of the composition and predicted functions of rumen microbiota in dry dairy cows from regional farms, which underscores the importance of the relationships among hosts, microbiota, diet composition, and milk production.

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Composition of Rumen Bacterial Community in Dairy Cows With Different Levels of Somatic Cell Counts

Cited by 53 publications

References 41 publications

Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis

Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis

Positive effects of dietary supplementation of three probiotics on milk yield, milk composition and intestinal flora in Sannan dairy goats varied in kind of probiotics

The Rumen Microbiome in Dry Dairy Cows Reveals the Specific Bacteriome Contributing to Various Physiological Roles and Phenotypes

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