Composition of the lipopolysaccharide of Neisseria gonorrhoeae

Wiseman, G. M.; Caird, J. D.

doi:10.1128/iai.16.2.550-556.1977

Cited by 34 publications

(14 citation statements)

References 18 publications

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“…Mo-xted to, grame drzakowski et al found numerous bactericidal ted to, gram-fractions from chromatographically fractionated ainca previous granule contents and went on to more comien amous pletely describe a fraction (valley AB from a cient amounts Sephadex G-100 column) that contained a po-T isedkibitegd tent bactericidal cationic protein with an appar-P5 M) present ent molecular weight of approximately 36,500 (6, ion by CCP of 7) Gram-negative bacteria, possessing lipopolyhio cOPacofi sacchande with the Ra chemotype, appeared to cherechla osli have similar sensitivities to Modrzakowski's valthereas loss of ley AB protein and Weiss's B/PI. The gonococci ilrty in gono-used in this report apparently have the Ra chemcurs only after d decrease in otype lipopolysaccharide (12,24) and appear to (iv) The bac-react to partially purified B/PI protein with the ted by heating same sensitivity as Ra mutants of E. coli (mangonococcicidal uscript in preparation). -0.179 (0.05) 0.580 (0.02) a At 0 min, the spectrophotometer was zeroed at 550 nm to the PBS control containing gonococci and was used as the blank for the other assays.…”

Section: Resultsmentioning

confidence: 77%

Effects of human neutrophil granule extracts on macromolecular synthesis in Neisseria gonorrhoeae

Buck¹,

Rest²

1981

Infect Immun

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Neisseria gonorrhoeae were exponentially killed for 120 min (i.e., they were prevented from forming colonies on agar) by extracts of human neutrophil granules; however, macromolecular synthesis, indicated by incorporation of radiolabeled precursors in trichloroacetic acid-precipitable material, continued at or above zero time control values for 45 min. Protein, deoxyribonucleic acid, and ribonucleic acid synthesis appeared to decrease simultaneously after 45 min. Little or no lysis of gonococci occurred during the first 60 min of incubation. The ions K+, Na+, Ca2+, Cll-, S042and PO43at concentrations of l100 mM did not affect granule extract bactericidal activity. On the other hand, 20 mM Mg2+ completely inhibited killing when initially present along with granule extract or when added within 2 to 5 min after granule extract was added to a suspension of gonococci. Gonococci treated with granule extract, washed, and then incubated in the absence of extract died as if extract were still present. The ability of subinhibitory concentrations of actinomycin D or erythromycin to inhibit growth and protein and nucleic acid synthesis was synergistically increased in the presence of granule extract. The above information suggests that a bactericidal component(s) of human neutrophil granules sticks to gonococci, altering their outer membrane permeability and their ability to divide. MATERIALS AND METHODS Bacteria. N. gonorrhoeae strain F62 was obtained

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Section: Resultsmentioning

confidence: 77%

Effects of human neutrophil granule extracts on macromolecular synthesis in Neisseria gonorrhoeae

Buck¹,

Rest²

1981

Infect Immun

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“…It has been shown that T1 variants may contain S type lipopolysaccharide (21), in addition to R type lipopolysaccharide (22,28), the only type found in T4 variants. Furthermore, T1 variants have a larger amount of acidic glycoses, N-acetylneuraminic acid and 2-ketodeoxyoctulosonic acid than T4 variants (30).…”

Section: Resultsmentioning

confidence: 95%

Effect of colony type and pH on surface charge and hydrophobicity of Neisseria gonorrhoeae

Magnusson¹,

Kihlström²,

Norlander³

et al. 1979

Infect Immun

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The effect of colonial variation and growth at pH 7.2 or pH 6.0 on the surface properties of Neisseria gonorrhoeae was assessed by the use of two-phase partitioning and hydrophobic interaction chromatography. Cells grown at pH 7.2 tended to be both hydrophobic and to possess a slight negative charge. Growth at pH 6.0 appeared to decrease hydrophobicity and to increase the negative surface charge. Possession of a series of outer membrane proteins, termed the colony opacity-associated proteins, did not appear to significantly affect charge or hydrophobicity. Piliated cells tended to have a higher negative charge than nonpiliated variants. They also tended to be less hydrophobic at pH 7.2, but became more hydrophobic at pH 6.0. The implications of these findings are discussed. to pH 6.0 with N-2-hydroxyethylpiperazine-N'-2-eth-397 on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

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“…minal tetrasaccharide (lacto-N-neotetraose, LNnT) of paragloboside (nLc4Cer), a glycosphingolipid precursor ofthe major human blood group antigens (10), and terminates in the disaccharide N-acetyllactosamine (LacNAc) . Although complete chemical structures have not been reported for the oligosaccharides of any gonococcal LOS, several laboratories have reported that the LOS of various strains were composed ofdifferent ratios ofthe same monosaccharides (Gal, Glc, G1cNAc, Hep, dOclA) as meningococcal LOSS (11)(12)(13)(14)(15)(16) .…”

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confidence: 99%

Lipooligosaccharides (LOS) of Neisseria gonorrhoeae and Neisseria meningitidis have components that are immunochemically similar to precursors of human blood group antigens. Carbohydrate sequence specificity of the mouse monoclonal antibodies that recognize crossreacting antigens on LOS and human erythrocytes.

Mandrell

Griffiss

Macher

1988

The Journal of Experimental Medicine

253

167

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We have used mouse mAbs, 3F11 and 06B4, that are specific for highly conserved epitopes of Neisseria gonorrhoeae lipooligosaccharides (LOS) to identify immunochemically similar structures on human erythrocytes. mAb 3F11 agglutinated erythrocytes from all randomly selected adult humans, while mAb 06B4 agglutinated only 80% of the same specimens. The antibodies had an activity with erythrocytes similar to human cold agglutinins in that agglutination occurred at 4 degrees C and decreased with increasing incubation temperature. Human infant erythrocytes were agglutinated less well, but enzymatic treatment of either infant or adult cells resulted in an increase in expression of the 3F11- and 06B4-defined epitopes. Both antibodies bound to a series of neutral glycosphingolipids from human erythrocytes and neutrophils that have a type 2 (Gal beta 1----4GlcNAc) or N-acetyllactosamine structure. Neither antibody bound to glycosphingolipids from human meconium, which have a type 1 (Gal beta 1----3GlcNAc) structure. The antibodies were unable to bind to N-acetyl-lactosamine glycosphingolipids with a nonreducing terminal sialic acid or a Gala1----3Gal disaccharide. Antibody binding also was blocked by the presence of fucose linked to the penultimate glucosamine residue of N-acetyllactosamine glycosphingolipids. Although both antibodies bound to linear and branched-chain N-acetyllactosamine glycosphingolipids, 3F11 had a higher affinity for branched structures than did 06B4. The activity of 3F11 with human adult and infant treated and untreated erythrocytes with N-acetyllactosamine glycosphingolipids, and with LOS was very similar, if not identical, in specificity to 1B2, an mAb prepared from mice inoculated with a linear N-acetyllactosamine glycosphingolipid.

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Composition of the lipopolysaccharide of Neisseria gonorrhoeae

Cited by 34 publications

References 18 publications

Effects of human neutrophil granule extracts on macromolecular synthesis in Neisseria gonorrhoeae

Effects of human neutrophil granule extracts on macromolecular synthesis in Neisseria gonorrhoeae

Effect of colony type and pH on surface charge and hydrophobicity of Neisseria gonorrhoeae

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