Composition of the microbial communities in the gastrointestinal tract of perch (Perca fluviatilis L. 1758) and cestodes parasitizing the perch digestive tract

Abstract

Using the approach of sequencing the V3‐V4 region of the 16S rRNA gene, we have analysed the bacterial diversity associated with the distinct compartments of the gastrointestinal tract of perch (Perca fluviatilis) and cestodes (Proteocephalus sp.) parasitizing their digestive tract. The dominant microbiota associated with cestodes (Proteocephalus sp.) was represented by bacteria from the genera Serratia, Pseudomonas and Mycoplasma. By comparing the associated microbiota of perch and cestodes, a clear difference in bacterial composition and diversity was revealed between the community from the stomach content and other parts of the gastrointestinal tract of fish. Microbiota associated with cestodes was not significantly different in comparison with microbiota of different subcompartments of perch (mucosa and content of intestine and pyloric caeca) (ADONIS, p > .05) excluding microbiota of stomach content (ADONIS, p ≤ .05). PICRUSt‐based functional assessments of the microbial communities of perch and cestodes indicated that they mainly linked in terms of metabolism and environmental information processing and could play an important role in the nutrition and health of host.

The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia)

“…Following the kit manufacturer protocols, DNA was extracted using the DNA-sorb B kit (NextBio, Russia). The DNA extraction protocol was previously described in Kashinskaya et al (2020) . DNA from a sample containing only sterile deionized water was extracted and included in PCR as a negative control.…”

“…Interactions between bacterial diversity found in the host and the microbes associated with the helminthes have been studied less extensively in fish. To date, it has been shown that different ecto- and endoparasites of fish are known to harbor a unique bacterial community ( Hughes-Stamm et al, 1999 ; Poddubnaya and Izvekova, 2005 ; Korneva and Plotnikov, 2006 ; Llewellyn et al, 2017 ; Gonçalves et al, 2020 ; Kashinskaya et al, 2020, 2021 ). In particular, a bacterial species was found to colonize the different areas of cestode teguments of Trianophorous nodulosus, Eubothrium rugosum and Ligula intestinalis from the intestine of pike, burbot, and bream, correspondingly ( Izvekova and Lapteva, 2004 ).…”

“…Most of these studies reported so far are focused on the morphological and physiological characteristics of bacteria associated with parasites inhabiting the intestine of fish ( Hughes-Stamm et al, 1999 ; Izvekova, 2003 ; Poddubnaya and Izvekova, 2005 ; Korneva and Plotnikov, 2006, 2012 ). There are only a few studies that have used V3–V4 16S rRNA sequencing technologies to create a metagenome library for analysis of the microbiota associated with tegument of cestodes and microbiota of ectoparasites ( Llewellyn et al, 2017 ; Gonçalves et al, 2020 ; Kashinskaya et al, 2020, 2021 ).…”

Microbial community structure in a host–parasite system: the case of Prussian carp and its parasitic crustaceans

“…The DNA extraction protocol was previously described in Kashinskaya et al . (2020). DNA from a sample containing only sterile deionized water was extracted and included in PCR as a negative control.…”

High-Starch Diet Consumption Impaired Intestinal Barriers of Juvenile Largemouth Bass (Micropterus Salmoides)

“…In present study, high starch diet feeding resulted in increasing relative abundance of Mycoplasmas, and there was a positively correlation was observed between relative abundance of mycoplasma with in ammatory(TNF-α, IL-1β, IL-8 and hepcidin1,2) and apoptotic(caspase-3,8,9 and bax) genes. Carbohydrates are the main source of energy for mycoplasma and converted to lactic acid and acetic acid, which are essential for immune and in ammation regulation (Brown, 2002;Kashinskaya et al, 2019). Furthermore, host immune system also plays a key role in shaping gut microbial communities (Kokou et al, 2019).…”