Abstract:In this study, the protein and total phenolic contents of some commercially cultivated (Agaricus bisporus, Pleurotus ostreatus) and wild mushrooms (Amanita caesarea, Fistulina hepatica, Meripilus giganteus) were determined. Antioxidant and antimicrobial properties of these mushrooms against Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Salmonella typhimurium, Acinetobacter haemolyticus, Proteus mirabilis, Pseudomonas aeruginosa and Candida albicans were also investigated. The protein contents, total phenolic contents and antioxidant activities of the mushrooms were found in the range of 11.00 -25.1%, 1.111 -3.858 mg GAE g -1 , and 1.528 -9.340 μmol FeSO4·7H2O g -1 , respectively. Meripilus giganteus had higher protein than all the tested mushrooms. The highest total phenolic content was detected in Agaricus bisporus obtained from B company (3.858 mg GAE g -1 ), whereas the lowest total phenolic content was observed in Meripilus giganteus (1.111 mg GAE g -1 ). Total phenolic and antioxidant properties of mushrooms were found significantly different (P<0.05) by Duncan's multiple range test. Methanolic extracts of the tested mushrooms showed no inhibitory activity against bacteria and yeast.