Compositional and structural dynamics of the ruminal microbiota in dairy heifers and its relationship to methane production

Cunha, Camila Soares; Marcondes, Marcos Inácio; Veloso, Cristina Mattos; Mantovani, Hilário Cuquetto; Pereira, L. G. R.; Tomich, T. R.; Dill‐McFarland, Kimberly A.; Suen, Garret

doi:10.1002/jsfa.9162

Cited by 24 publications

(22 citation statements)

References 48 publications

(90 reference statements)

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“…Both phyla are commonly found in the feces of adult cattle, with Firmicutes commonly being the predominate phylum [6,42]. Most studies of Methanobrevibacter have shown that it is the most common methanogen in the rumen of cattle [43,44], but its role in the fecal microbiota is unclear. Interestingly, reducing this organism could reduce methane production in treated animals and improve feed efficiency [43].…”

Section: Discussionmentioning

confidence: 99%

Ceftiofur formulation differentially affects the intestinal drug concentration, resistance of fecal Escherichia coli, and the microbiome of steers

et al. 2019

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Antimicrobial drug concentrations in the gastrointestinal tract likely drive antimicrobial resistance in enteric bacteria. Our objective was to determine the concentration of ceftiofur and its metabolites in the gastrointestinal tract of steers treated with ceftiofur crystalline-free acid (CCFA) or ceftiofur hydrochloride (CHCL), determine the effect of these drugs on the minimum inhibitory concentration (MIC) of fecal Escherichia coli, and evaluate shifts in the microbiome. Steers were administered either a single dose (6.6 mg/kg) of CCFA or 2.2 mg/kg of CHCL every 24 hours for 3 days. Ceftiofur and its metabolites were measured in the plasma, interstitium, ileum and colon. The concentration and MIC of fecal E. coli and the fecal microbiota composition were assessed after treatment. The maximum concentration of ceftiofur was higher in all sampled locations of steers treated with CHCL. Measurable drug persisted longer in the intestine of CCFA-treated steers. There was a significant decrease in E. coli concentration (P = 0.002) within 24 hours that persisted for 2 weeks after CCFA treatment. In CHCL-treated steers, the mean MIC of ceftiofur in E. coli peaked at 48 hours (mean MIC = 20.45 ug/ml, 95% CI = 10.29–40.63 ug/ml), and in CCFA-treated steers, mean MIC peaked at 96 hours (mean MIC = 10.68 ug/ml, 95% CI = 5.47–20.85 ug/ml). Shifts in the microbiome of steers in both groups were due to reductions in Firmicutes and increases in Bacteroidetes. CCFA leads to prolonged, low intestinal drug concentrations, and is associated with decreased E. coli concentration, an increased MIC of ceftiofur in E. coli at specific time points, and shifts in the fecal microbiota. CHCL led to higher intestinal drug concentrations over a shorter duration. Effects on E. coli concentration and the microbiome were smaller in this group, but the increase in the MIC of ceftiofur in fecal E. coli was similar.

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Section: Discussionmentioning

confidence: 99%

Ceftiofur formulation differentially affects the intestinal drug concentration, resistance of fecal Escherichia coli, and the microbiome of steers

et al. 2019

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“…As ammonia production in the rumen consumes H 2 gas, Eubacterium spp. might play a role in decreasing CH 4 emissions by depriving methanogens of H 2 [13,48]. The relation between the decreasing amount of GS and the unclassified group belonging to the Saccharibacteria and the YAB-2003 group belonging to the Prevotellaceae is unclear due to the limited knowledge with respect to the physiology of these organisms.…”

Section: Effect Of Diet and Time On Rumen Bacterial Compositionmentioning

confidence: 99%

Characterization of dairy cow rumen bacterial and archaeal communities associated with grass silage and maize silage based diets

et al. 2020

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The objective of the present study was to characterize the rumen bacterial and archaeal communities in dairy cows fed different ratios of maize silage (MS) and grass silage (GS), and place the findings in the context of ruminal fermentation as well as previously reported methane (CH 4) emissions. Rumen fluid from 12 rumen cannulated dairy cows was collected after 10 and 17 days of feeding one of four diets, all of which had the same roughage to concentrate ratio of 80:20 based on dry matter (DM). Roughage in the four diets (GS100, GS0, GS67, GS33) consisted of either 1000 g/kg DM GS (GS100), 1000 g/kg DM MS (GS0), or a mixture of both silages in different proportions [667 g/kg DM GS and 333 g/kg DM MS (GS67); 333 g/kg DM GS and 677 g/kg DM MS (GS33)]. Total volatile fatty acid (VFA) concentrations and the molar proportions of the ruminal VFA were not affected by diet. Only the molar proportion of isovalerate was affected by time, being lower on day 17 than on day 10. Bacterial and archaeal concentrations were not affected by diet but increased from day 10 to day 17. The bacterial community composition was affected by diet, time and diet × time, whereas the archaeal community composition was only affected by diet. Several bacterial and archaeal genus level groups were associated with diet, but not with time. Analysis indicated the increased use of hydrogen by succinate and lactate producing bacteria is likely to at least partially explain the previously reported lower CH 4 emissions from MS fed dairy cows. Furthermore, time had a significant effect on both bacterial and archaeal concentrations, and also bacterial community composition. This indicates that the rumen microbiota had not stabilized after 10 days of feeding the experimental diets.

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“…All of them are short chain fatty acids important to the glucose biosynthesis in cattle [2]. Lastly, the family BF16 is also a rumen-native taxon with higher abundance in mature calves and also correlated to methane production [42,43]. All of these taxa were previously reported as members of the regular and efficient microbiota from rumen and their increased abundance may indicate an improved ability to digestion or, at least, a need for more specialized fermentation in rumen due to, for example, more food intake.…”

Section: Discussionmentioning

confidence: 99%

Microbial Patterns in Rumen Are Associated with Gain of Weight in Beef Cattle

Freitas

David

Takagaki

et al. 2020

Preprint

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Ruminal microorganisms play a pivotal role in cattle nutrition. The discovery of the main microbes responsible for enhancing the gain of weight in beef cattle might be used in therapeutic approaches to increase animal performance and cause less environmental damages. Here, we examined differences in bacterial and fungal composition of rumen samples of Braford heifers raised in a natural grassland from Pampa Biome in Brazil. We aimed to detect microbial patterns in the rumen that could be correlated with the gain of weight. 16S and ITS1 genes were amplified from ruminal samples and sequenced to identify the closest microbial relatives within the microbial communities. A predictive model based on microbes responsible for the gain of weight was build and further tested using the entire dataset. The model detected a set of microorganisms associated with animals in the high gain of weight group, including the bacterial taxa RFN20, Prevotella, Anaeroplasma and RF16 and the fungal taxa Aureobasidium, Cryptococcus, Sarocladium, Pleosporales and Tremellales. Most of these organisms have been correlated to the production of substances that improve the ruminal digestion process. These findings provide new insights about cattle nutrition and suggest the use of these microbes to improve beef cattle breeding.

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Compositional and structural dynamics of the ruminal microbiota in dairy heifers and its relationship to methane production

Abstract: We suggest that Eubacterium and Methanosphaera represent likely targets for CH mitigation efforts in heifers as they were negatively associated with CH production and not significantly associated with production traits. © 2018 Society of Chemical Industry.

Cited by 24 publications

References 48 publications

Ceftiofur formulation differentially affects the intestinal drug concentration, resistance of fecal Escherichia coli, and the microbiome of steers

Ceftiofur formulation differentially affects the intestinal drug concentration, resistance of fecal Escherichia coli, and the microbiome of steers

Characterization of dairy cow rumen bacterial and archaeal communities associated with grass silage and maize silage based diets

Microbial Patterns in Rumen Are Associated with Gain of Weight in Beef Cattle

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