Compositional and Thermal Properties of Thylakoid Polar Lipids of <i>Nerium oleander</i> L. in Relation to Chilling Sensitivity

Orr, Glenda R.; Raison, John K.

doi:10.1104/pp.84.1.88

Cited by 32 publications

(14 citation statements)

References 8 publications

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“…This involves enzymes that tailor lipids in order to ensure optimal mobility for membranous molecules, and hence optimal efficiency of the membrane-associated photosynthetic processes [36,42]. It was recognized that the overall fluidity of the membrane cannot be simply predicted from the saturated fatty acid contents and phase transition temperatures of the component lipids [43]. Membrane fluidity also depends largely on lipid composition.…”

Section: Membrane Fluidity and The Lipid-protein Interfacementioning

confidence: 99%

Functional significance of the lipid-protein interface in photosynthetic membranes

Páli

Garab

Horváth

et al. 2003

Cellular and Molecular Life Sciences (CMLS)

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The functional significance of the lipid-protein interface in photosynthetic membranes, mainly in thylakoids, is reviewed with emphasis on membrane structure and dynamics. The lipid-protein interface is identified primarily by the restricted molecular dynamics of its lipids as compared with the dynamics in the bulk lipid phase of the membrane. In a broad sense, lipid-protein interfaces comprise solvation shell lipids that are weakly associated with the hydrophobic surface of transmembrane proteins but also include lipids that are strongly and specifically bound to membrane proteins or protein assemblies. The relation between protein-associated lipids and the overall fluidity of the thylakoid membrane is discussed. Spin label electron paramagnetic resonance spectroscopy has been identified as the technique of choice to characterize the protein solvation shell in its highly dynamic nature; biochemical and direct structural methods have revealed an increasing number of protein-bound lipids. The structural and functional roles of these protein-bound lipids are mustered, but in most cases they remain to be determined. As suggested by recent data, the interaction of the non-bilayer-forming lipid, monogalactosyldyacilglycerol (MGDG), with the main light-harvesting chlorophyll a/b-binding protein complexes of photosystem-II (LHCII), the most abundant lipid and membrane protein components on earth, play multiple structural and functional roles in developing and mature thylakoid membranes. A brief outlook to future directions concludes this review.

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Section: Membrane Fluidity and The Lipid-protein Interfacementioning

confidence: 99%

Functional significance of the lipid-protein interface in photosynthetic membranes

Páli

Garab

Horváth

et al. 2003

Cellular and Molecular Life Sciences (CMLS)

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“…Raison and Orr, 1990). The primary event which is physical in nature and temperature dependent is related to the temperature of the transition of polar Iipids and greatly influenced by the proportion ofhtm fraction of PG (Raison and Wright 1983;Murata and Yamaya, 1984;Orr and Raison 1987) and correlated with the critical temperature for photosynthesis (Hodgson et al 1987;Raison and Brown, 1989). Measurements of the phase transition temperature and critical temperature for photosynthesis when applied for comparison of closely related species, as in the case of three altitudinal ecotypes of the Licopersicon hirsutum enabled to state that all these ecotypes are chilling sensitive (Raison and Brown, 1989).…”

Section: Chilling Sensitivity Versus Response To Chillingmentioning

confidence: 99%

Degradation of leaf polar lipids during chilling and post-chilling rewarming of Zea mays genotypes reflects differences in their response to chilling stress. The role of galactolipase

et al. 1999

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Degradation of leaf polar lipids [monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulfoquinovosyldiacylglycerol (SQDG) and phosphatidytglycerol (PG)J and chlorophyll (Chl) were studied in four Zea mays genotypes differing in chilling susceptibility following dark chilling and post-chilling rewarming at original growth conditions. Assessment of visual chilling injury symptoms during post-chilling rewarming differentiated maize inbred lines into chiling-sensitive (CS) CM7 and Co151 lines and chillingtolerant (CT) $215 and EP1 lines. Severity of chilling injury in CS and CT inbreeds were correlated with the extent of Chl and polar lipids degradation.Chilling for either 4 or 6 days followed by 4 days of rewarming caused more extensive degradation of total polar lipids content in CS than in CT lines. MGDG decreased rnostly during chilling whereas DGDG dropped during rewarming only. Chl content was not affected during chilling but its large decrease, greater in CS than in CT lines, was observed upon rewarming. Extent of polar lipids breakdown in CS and CT inbreeds during chilling and post-chilling rewarming is correlated with galactolipase activity in chloroplasts (Kaniuga et al., 1998) and visual assesment of chilling injury.In view of the data it is likely that contribution of galactolipase activity induced during low-temperature stress of CS plants is an important factor responsible lk~r thylakoid lipid degradation and development of chilling injury as postulated previously (Kaniuga I997). It is suggested that genetically engineered reduction of galactolipase activity or elimination of the factors(s) involved in induction/stimulation of its activity during chilling might increase tolerance of CS species to chilling stress.

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“…2, 3). Previous studies had also indicated the transition is more likely the solidification of a mixture of some high-and low-melting-point lipids (Raison and Wright 1983;Orr and Raison 1987). The most likely high-melting-point components in the mixture which solidifies at the transition temperature would be some of the saturated molecular species of PG and SQDG which, for oleander, have transition temperatures within the range 19 ~ to 26 ~ C , and for the few chilling-sensitive, tropical, plants studied, the range is 29 ~ to 32~ (Murata and Yamaya 1984).…”

Section: Discussionmentioning

confidence: 96%

“…Polar lipids of oleander and cucumber thylakoids were extracted, purified and separated into individual classes as described by Orr and Raison (1987). The amount of galactolipids was estimated by sugar analysis (Roughan and Batt 1968) and that of phospholipids by phosphorus analysis (Ames 1966).…”

Section: Methodsmentioning

confidence: 99%

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The effect of changing the composition of phosphatidylglycerol from thylakoid polar lipids of oleander and cucumber on the temperature of the transition related to chilling injury

Orr

Raison

1990

Planta

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The composition and phase behavior of some lipid classes and mixtures of thylakoid polar lipids were measured to investigate their role as determinants of the temperature of the transition associated with chilling injury. For Nerium oleander L., a plant which acclimates to growth temperature, a mixture of the phosphatidylglycerol (PG) and sulfoquinovosyldiacylglycerol (SQDG) showed transition temperatures of 22° and 10° C for plants grown at 45° and 20° C, respectively. This difference was similar to the 9 Celsius degrees differential in the transition of the polar lipids and indicated that the PG and-or the PG-SQDG mixture could be the major determinants of the transition temperature. Reconstitution of the PG-SQDG mixture from 20°-grown oleander with the galactolipids from 45°-grown plants, however, reduced the transition temperature by only 4 Celsius degrees. This indicates that some, low-melting-point lipids, which are structurally capable of forming a co-gel with the high-melting-point lipids, also play a role in determining the temperature of the transition and that the composition of these low-melting-point lipids also changes with growth temperature. More specific information on the role of PG was obtained using polar lipids from Cucumis sativus L., a chilling-sensitive plant. For this material the transition in the polar lipids was reduced from 9° to 5° and 4° C when the transition of the PG was reduced from 32° to 25° and 22° C. This was accomplished by reducing the proportion of disaturated molecular species in PG from 78 to 56 and 44 mol% by the addition of a fraction of the PG enriched in unsaturated molecular species. The data indicate that the transition temperature of the polar lipids of cucumber would be reduced to below 0° C, typical of a chillinginsensitive plant, when the transition temperature of PG was reduced to 15° C and this would occur at 21 mol% of disaturated molecular species. It is concluded that the transition in the thylakoid polar lipids, associated with chilling injury, involves both high- and low-meltingpoint lipids but can be reduced when the transition temperature of the high-melting-point component is reduced.

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Compositional and Thermal Properties of Thylakoid Polar Lipids of Nerium oleander L. in Relation to Chilling Sensitivity

Cited by 32 publications

References 8 publications

Functional significance of the lipid-protein interface in photosynthetic membranes

Functional significance of the lipid-protein interface in photosynthetic membranes

Degradation of leaf polar lipids during chilling and post-chilling rewarming of Zea mays genotypes reflects differences in their response to chilling stress. The role of galactolipase

The effect of changing the composition of phosphatidylglycerol from thylakoid polar lipids of oleander and cucumber on the temperature of the transition related to chilling injury

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