Compositional characterisation of soluble apple polysaccharides, and their antioxidant and hepatoprotective effects on acute CCl4-caused liver damage in mice

Characterization, antioxidant activities and hepatoprotective effects of polysaccharides from pre-pressing separationFujiapple peel

“…Pre-treatment with positive 450 mg/kg bw of BP showed similar decreasing effects on both the indexes ( Table 1), indicating that the administration of APP to mice may generate a comparable preventive effect against CCl 4 -induced damage as BP. Our results presented consist with the reported observations (Wang et al, 2012;Yang et al, 2013).…”

“…Nine kinds of standard monosaccharides derived with PMP could be separated well through the column within 25 min. As shown, APP was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose and arabinose, and their corresponding mole percentages were 10.5%, 9.2%, 5.3%, 18.5%, 6.5%, 7.2%, 20.3% and 21.5% of all the quantitative monosaccharides, respectively, which was similar to the results obtained by Yang et al (2013), even though the polysaccharides tested were from two different varieties (Fuji and Pink Lady, respectively). Both kinds of polysaccharides contained some parts of pectin.…”

“…The monosaccharide composition of APP was analysed using HPLC method established by Yang et al (2013) with some modifications. Specifically, 20 mg APP was hydrolysed in a 5-mL sealed ampoule by the addition of 2.0 mL of 3 M trifluoroacetic acid (TFA) at 95°C for 8 h to fully release the constitutive monosaccharides.…”

“…## p < 0,01, significativamente diferente en comparación con el grupo normal. *p < 0,05 **p < 0,01, significativamente diferente en comparación con el grupo intoxicado con CCl 4. mole percentage of uronic acids (23.8% and 17.7% for Fuji and Pink Lady, respectively) that have been shown to play an important role in polysaccharides bioactivities (Chen, Zhang, & Xie, 2004;Yang et al, 2013).…”

“…Therefore, natural antioxidant phytochemicals have recently been shown a substantial increase as alternative therapies of liver diseases because of better effectiveness and fewer side effects (Jiang et al, 2013). Recently, antioxidant extracts from Pink Lady apples were found to possess hepatoprotective effects against CCl 4 -induced liver injury in mice (Yang, Li, Wang, & Wu, 2010;Yang, Yang, Guo, Jiao, & Zhao, 2013). Fuji apple is one of the most popular apple cultivars in the world (Murata, Tsurutani, Tomita, Homma, & Kaneko, 1995).…”