Compositional, Structural, and Kinetic Aspects of Lipid Digestion and Bioavailability: In Vitro, In Vivo, and Modeling Approaches

Marze, Sébastien

doi:10.1146/annurev-food-052720-093515

Cited by 3 publications

(3 citation statements)

References 142 publications

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“…Consequently, the abilities of cells to rapidly move various nutrients, structural components and newly synthesized molecules to where they are needed intracellularly and to remove them if they are damaged and no longer needed are essential to cell, tissue and organ health. This normal trafficking of cellular molecules is especially important and crucial for membrane lipids [ 73 , 74 , 75 , 76 , 77 , 78 ].…”

Section: Movements Of Lipids Into/out Of Cells: Lipid Carriers Vesicl...mentioning

confidence: 99%

“…Here we expand briefly on some of the relationships between dietary membrane lipids and various cellular membranes, focusing on the movements of membrane lipids between different membranes and the removal of damaged membrane lipids from cells. First, it has been established that dietary lipid sources can be used to drive the replacement of damaged membrane lipids, even though it is usually impossible to consume sufficient quantities of membrane lipids in foods to fulfill this replacement by diet alone [ 78 ]. Dietary lipids, including membrane lipids, are ingested, digested, absorbed by epithelial cells in the upper small intestines and then transferred and transported via lymph and blood circulation to the liver and to various organs and tissues to be absorbed again.…”

Section: Movements Of Lipids Into/out Of Cells: Lipid Carriers Vesicl...mentioning

confidence: 99%

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Fifty Years of the Fluid–Mosaic Model of Biomembrane Structure and Organization and Its Importance in Biomedicine with Particular Emphasis on Membrane Lipid Replacement

Nicolson

Ferreira

2022

Biomedicines

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The Fluid–Mosaic Model has been the accepted general or basic model for biomembrane structure and organization for the last 50 years. In order to establish a basic model for biomembranes, some general principles had to be established, such as thermodynamic assumptions, various molecular interactions, component dynamics, macromolecular organization and other features. Previous researchers placed most membrane proteins on the exterior and interior surfaces of lipid bilayers to form trimolecular structures or as lipoprotein units arranged as modular sheets. Such membrane models were structurally and thermodynamically unsound and did not allow independent lipid and protein lateral movements. The Fluid–Mosaic Membrane Model was the only model that accounted for these and other characteristics, such as membrane asymmetry, variable lateral movements of membrane components, cis- and transmembrane linkages and dynamic associations of membrane components into multimolecular complexes. The original version of the Fluid–Mosaic Membrane Model was never proposed as the ultimate molecular description of all biomembranes, but it did provide a basic framework for nanometer-scale biomembrane organization and dynamics. Because this model was based on available 1960s-era data, it could not explain all of the properties of various biomembranes discovered in subsequent years. However, the fundamental organizational and dynamic aspects of this model remain relevant to this day. After the first generation of this model was published, additional data on various structures associated with membranes were included, resulting in the addition of membrane-associated cytoskeletal, extracellular matrix and other structures, specialized lipid–lipid and lipid–protein domains, and other configurations that can affect membrane dynamics. The presence of such specialized membrane domains has significantly reduced the extent of the fluid lipid membrane matrix as first proposed, and biomembranes are now considered to be less fluid and more mosaic with some fluid areas, rather than a fluid matrix with predominantly mobile components. However, the fluid–lipid matrix regions remain very important in biomembranes, especially those involved in the binding and release of membrane lipid vesicles and the uptake of various nutrients. Membrane phospholipids can associate spontaneously to form lipid structures and vesicles that can fuse with various cellular membranes to transport lipids and other nutrients into cells and organelles and expel damaged lipids and toxic hydrophobic molecules from cells and tissues. This process and the clinical use of membrane phospholipid supplements has important implications for chronic illnesses and the support of healthy mitochondria, plasma membranes and other cellular membrane structures.

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Section: Movements Of Lipids Into/out Of Cells: Lipid Carriers Vesicl...mentioning

confidence: 99%

Section: Movements Of Lipids Into/out Of Cells: Lipid Carriers Vesicl...mentioning

confidence: 99%

Fifty Years of the Fluid–Mosaic Model of Biomembrane Structure and Organization and Its Importance in Biomedicine with Particular Emphasis on Membrane Lipid Replacement

Nicolson

Ferreira

2022

Biomedicines

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“…Mathematical modeling of in vitro lipid digestion kinetics has been investigated both in the field of food and pharmaceutical sciences [20,[28][29][30]. Some of the earlier investigations of lipid digestion kinetics use Michaelis-Menten kinetics to model lipid hydrolysis (e.g., [28]).…”

Section: Introductionmentioning

confidence: 99%

Intrinsic lipolysis rate for systematic design of lipid-based formulations

Jacobsen

Kabedev

Sinko

et al. 2022

Drug Deliv. and Transl. Res.

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Lipid-based formulations (LBFs) are used by the pharmaceutical industry in oral delivery systems for both poorly water-soluble drugs and biologics. Digestibility is key for the performance of LBFs and in vitro lipolysis is commonly used to compare the digestibility of LBFs. Results from in vitro lipolysis experiments depend highly on the experimental conditions and formulation characteristics, such as droplet size (which defines the surface area available for digestion) and interfacial structure. This study introduced the intrinsic lipolysis rate (ILR) as a surface area-independent approach to compare lipid digestibility. Pure acylglycerol nanoemulsions, stabilized with polysorbate 80 at low concentration, were formulated and digested according to a standardized pH–stat lipolysis protocol. A methodology originally developed to calculate the intrinsic dissolution rate of poorly water-soluble drugs was adapted for the rapid calculation of ILR from lipolysis data. The impact of surfactant concentration on the apparent lipolysis rate and lipid structure on ILR was systematically investigated. The surfactant polysorbate 80 inhibited lipolysis of tricaprylin nanoemulsions in a concentration-dependent manner. Coarse-grained molecular dynamics simulations supported these experimental observations. In the absence of bile and phospholipids, tricaprylin was shielded from lipase at 0.25% polysorbate 80. In contrast, the inclusion of bile salt and phospholipid increased the surfactant-free area and improved the colloidal presentation of the lipids to the enzyme, especially at 0.125% polysorbate 80. At a constant and low surfactant content, acylglycerol digestibility increased with decreasing acyl chain length, decreased esterification, and increasing unsaturation. The calculated ILR of pure acylglycerols was successfully used to accurately predict the IRL of binary lipid mixtures. The ILR measurements hold great promise as an efficient method supporting pharmaceutical formulation scientists in the design of LBFs with specific digestion profiles. Graphical abstract

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Optimizing anthocyanin Oral delivery: Effects of food biomacromolecule types on Nanocarrier performance for enhanced bioavailability

Yuan,

Hu,

Huang

et al. 2024

Food Chemistry

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Compositional, Structural, and Kinetic Aspects of Lipid Digestion and Bioavailability: In Vitro, In Vivo, and Modeling Approaches

Cited by 3 publications

References 142 publications

Fifty Years of the Fluid–Mosaic Model of Biomembrane Structure and Organization and Its Importance in Biomedicine with Particular Emphasis on Membrane Lipid Replacement

Fifty Years of the Fluid–Mosaic Model of Biomembrane Structure and Organization and Its Importance in Biomedicine with Particular Emphasis on Membrane Lipid Replacement

Intrinsic lipolysis rate for systematic design of lipid-based formulations

Optimizing anthocyanin Oral delivery: Effects of food biomacromolecule types on Nanocarrier performance for enhanced bioavailability

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