Compositional variances in cuticular lipids of wild and domesticated barley leaves and their impact on plant-environment interactions

Sarkar, Sutanni; Arya, Gulab Chand; Negin, Boaz; Manasherova, Ekaterina; Levy, Maggie; Aharoni, Asaph; Cohen, Hagai

doi:10.1016/j.envexpbot.2022.105140

Cited by 9 publications

(4 citation statements)

References 69 publications

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“…4 ), and used the two Capsicum annuum endogenous genes Polyubiquitin-like (CA00g79660) and UBI-3 (Capana04g002411) as normalizing genes as described by [ 87 ]. The Fast SYBR™ Green Master Mix was used to amplify cDNA samples that were analyzed by a qRT-PCR machine (Applied Biosystems by Thermo Fisher Scientific) according to the thermal cycle program recently described by [ 88 ].…”

Section: Methodsmentioning

confidence: 99%

Microscopic and metabolic investigations disclose the factors that lead to skin cracking in chili-type pepper fruit varieties

Marinov

Nomberg

Sarkar

et al. 2023

Horticulture Research

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The hydrophobic cuticle encasing the fruit skin surface plays critical roles during fruit development and post-harvest. Skin failure often results in the fruit surface cracking and forming a wound-periderm tissue made of suberin and lignin. The factors that make the fruit skin susceptible to cracking have yet to be fully understood. Herein, we investigated two varieties of chili peppers (Capsicum annuum L.), Numex Garnet, whose fruit has intact skin, and Vezena Slatka, whose fruit has cracked skin. Microscopical observations, gas chromatography-mass spectrometry, biochemical and gene expression assays revealed that Vezena Slatka fruit form a thicker cuticle with greater levels of cutin monomers and hydroxycinnamic acids, and highly express key cutin-related genes. The skin of these fruit also had a lower epidermal cell density due to cells with very large perimeters, and highly express gene involved in epidermal cell differentiation. We demonstrate that skin cracking in the Vezena Slatka fruit is accompanied by a spatial accumulation of lignin-like polyphenolic compounds, without the formation of a typical wound-periderm tissues made of suberized cells. Lastly, we establish that skin cracking in chili-type pepper significantly affects fruit quality during post-harvest storage in a temperature-dependent manner. In conclusion, our data highlight cuticle thickness and epidermal cell density as two critical factors determining fruit skin susceptibility to cracking in chili-type pepper fruit.

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Section: Methodsmentioning

confidence: 99%

Microscopic and metabolic investigations disclose the factors that lead to skin cracking in chili-type pepper fruit varieties

Marinov

Nomberg

Sarkar

et al. 2023

Horticulture Research

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“…We further explored the possible involvement of EVER in wax biosynthesis in petunia flowers, having obtained the following pieces of evidence suggesting this link: (1) the homologs of EVER in Arabidopsis were shown to regulate the biosynthetic pathways of waxes; (2) our RNA-Seq data of EVER-knockout and overexpressing lines revealed enrichment of genes involved in wax-biosynthesis processes. We extracted the epicuticular waxes from 2DPA petals of ever #1 and #2 and control Cas9 lines and profiled their composition via GC-MS-based protocols (Sarkar et al, 2023). We positively identified 29 wax-related components, most of which belonged to wax esters previously shown to dominate epicuticular waxes in petunia flowers (King et al, 2007;Chen et al, 2017).…”

Section: The Petals Of Ever Knockouts Display Altered Composition Of ...mentioning

confidence: 99%

“…Chloroform extracts were then treated, derivatized and analyzed by GC-MS as recently described by Sarkar et al (2023).…”

Section: Epicuticular Wax Extraction and Analysismentioning

confidence: 99%

“…Cutin is a mixture of very-long-chain C16 and C18 fatty acid derivatives with terminal and mid-chain hydroxy, epoxy and carboxy functional groups (Cohen et al, 2019;Philippe et al, 2020). Intracuticular waxes are dispersed within the cutin matrix, and epicuticular waxes are deposited on top of the cuticle (Arya et al, 2021;Sarkar et al, 2023). Waxes consist of very-long-chain fatty acids (VLCFAs, typically >C20), and their derivatives belonging to various biochemical classes, including primary and secondary alcohols, aldehydes, alkanes, ketones and esters (Samuels et al, 2008;Buschhaus and Jetter, 2011).…”

Section: Introductionmentioning

confidence: 99%

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The R2R3-MYB transcription factor EVER controls the emission of petunia floral volatiles by regulating epicuticular wax biosynthesis in the petal epidermis

Skaliter,

Bednarczyk,

Shor

et al. 2023

The Plant Cell

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The epidermal cells of petunia (Petunia × hybrida) flowers are the main site of volatile emission. However, the mechanisms underlying the release of volatiles into the environment are still being explored. Here, using cell-layer-specific transcriptomic analysis, reverse genetics by virus-induced gene silencing and clustered regularly interspaced short palindromic repeat (CRISPR), and metabolomics, we identified EPIDERMIS VOLATILE EMISSION REGULATOR (EVER)—a petal adaxial epidermis-specific MYB activator that affects the emission of volatiles. To generate ever knockout lines, we developed a viral-based CRISPR/Cas9 system for efficient gene editing in plants. These knockout lines, together with transient-suppression assays, revealed EVER's involvement in the repression of low-vapor-pressure volatiles. Internal pools and annotated scent-related genes involved in volatile production and emission were not affected by EVER. RNA-Seq analyses of petals of ever knockout lines and EVER-overexpressing flowers revealed enrichment in wax-related biosynthesis genes. Liquid chromatography/gas chromatography–MS analyses of petal epicuticular waxes revealed substantial reductions in wax loads in ever petals, particularly of monomers of fatty acids and wax esters. These results implicate EVER in the emission of volatiles by fine-tuning the composition of petal epicuticular waxes. We reveal a petunia MYB regulator that interlinks epicuticular wax composition and volatile emission, thus unraveling a regulatory layer in the scent-emission machinery in petunia flowers.

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The dominant white color trait of the melon fruit rind is associated with epicuticular wax accumulation

Ezer,

Manasherova,

Gur

et al. 2024

Planta

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Compositional variances in cuticular lipids of wild and domesticated barley leaves and their impact on plant-environment interactions

Cited by 9 publications

References 69 publications

Microscopic and metabolic investigations disclose the factors that lead to skin cracking in chili-type pepper fruit varieties

Microscopic and metabolic investigations disclose the factors that lead to skin cracking in chili-type pepper fruit varieties

The R2R3-MYB transcription factor EVER controls the emission of petunia floral volatiles by regulating epicuticular wax biosynthesis in the petal epidermis

The dominant white color trait of the melon fruit rind is associated with epicuticular wax accumulation

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