Compounds for Control of Orange Decays

Childs, J. F. L.; Siegler, E. A.

doi:10.1126/science.102.2638.68

Cited by 26 publications

(5 citation statements)

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“…Thioacetamide was developed as an effective pesticide to control the decay of citrus fruits (Childs and Siegler, 1945), but was soon found to cause liver diseases (fibrosis and cirrhosis) and liver tumors (Fitzhugh and Nelson, 1948). It has been used extensively in animal studies (Ledda-Columbano et al, 1991; Li et al, 2002; Yeh et al, 2004; Okuyama et al, 2005; Dwivedi and Jena, 2018), largely for its ability to cause acute liver damage (Li et al, 2002; Okuyama et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Assessing Chemical-Induced Liver Injury In Vivo From In Vitro Gene Expression Data in the Rat: The Case of Thioacetamide Toxicity

et al. 2019

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Consumers are exposed to thousands of chemicals with potentially adverse health effects. However, these chemicals will never be tested for toxicity because of the immense resources needed for animal-based (in vivo) toxicological studies. Today, there are no viable in vitro alternatives to these types of animal studies. To develop an in vitro approach, we investigated whether we could predict in vivo organ injuries in rats with the use of RNA-seq data acquired from tissues early in the development of toxicant-induced injury, by comparing gene expression data from RNA isolated from these rat tissues with those obtained from in vitro exposure of primary liver and kidney cells. We collected RNA-seq data from the liver and kidney tissues of Sprague-Dawley rats 8 or 24 h after exposing them to vehicle (control), low (25 mg/kg), or high (100 mg/kg) doses of thioacetamide, a known liver toxicant that promotes fibrosis; we used these doses and exposure times to cause only mild toxicant-induced injury. For the in vitro study, we treated two cell types from Sprague-Dawley rats, primary hepatocytes (vehicle; low, 0.025 mM; or high, 0.125 mM dose), and renal tube epithelial cells (vehicle; low, 0.125 mM; or high, 0.500 mM) dose) with the thioacetamide metabolite, thioacetamide-S-oxide, selecting in vitro doses and exposure times to recreate the early-stage toxicant-induced injury model that we achieved in vivo. RNA-seq data were collected 9 or 24 h after application of vehicle or thioacetamide-S-oxide. We found that our modular approach for the analysis of gene expression data derived from in vivo RNA-seq strongly correlated (R2 > 0.6) with the in vitro results at two different dose levels of thioacetamide/thioacetamide-S-oxide after 24 h of exposure. The top-ranked liver injury modules in vitro correctly identified the ensuing development of liver fibrosis.

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Section: Introductionmentioning

confidence: 99%

Assessing Chemical-Induced Liver Injury In Vivo From In Vitro Gene Expression Data in the Rat: The Case of Thioacetamide Toxicity

et al. 2019

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“…Thioacetamide [TAA, CH 3 -C(S)NH 2 ], a hepatoxin, was first used to control the decay of oranges and then as a fungicide [1] . In the liver, TAA is S-oxidized at the thioamide group to TAA sulfoxide [CH 3 -C(SO)NH 2 ] and subsequently to di-Soxide [CH 3 -C(SO 2 )NH 2 ].…”

Section: Introductionmentioning

confidence: 99%

Co-administration of cyclosporine A alleviates thioacetamide-induced liver injury

Fan

Weng²

2005

WJG

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AIM:To investigate the effects of cyclosporine A (CsA) on thioacetamide (TAA)-induced liver injury. METHODS:CsA was co-administrated (7.5 g/kg body weight per day, i.p.) into rat to investigate the role of CsA on TAA-(200 mg/kg body weight per 3 d for 30 d, i.p.) induced liver injury. RESULTS:The data show that TAA caused liver fibrosis in rat after 30 d of treatment. CsA alleviates the morphological changes of TAA-induced fibrosis in rat liver. The blood glutamyl oxaloacetic transaminase (GOT)/glutamyl pyruvic transaminase (GPT) in the TAA-injury group is elevated compared to that of the normal rat. Compared with the TAA-injury group, the blood GOT/GPT and TGF1 (by RT-PCR analysis) are reduced in the CsA plus TAA-treated rat. The level of the transforming growth factor receptor I (TGF-R1) in the CsA plus TAA-treated group shows higher than that in the TAA only group, but shows a lower level of the fibroblast growth factor receptor 4 (FGFR4) in the CsA plus TAA-treated group, when using the Western blot analysis. After immunostaining of the frozen section, TGF-R1 and FGFR4 are more concentrated in rat liver after CsA plus TAA injury. CONCLUSION:This result suggests that CsA has an alleviated effect on TAA-induced liver injury by increasing the multidrug resistance P-glycoprotein and could be through the regulation of TGF-R1 and FGFR4.

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“…It was first used of decay of oranges and then as a fungicide. 19 Thioacetamide (TAA) is an organosulfur compound having formulation C2H5NS. 20 Several corporations and investigators studied the toxicity of thioacetamide like Fitzhugh and Nelson 1948.…”

Section: Thioacetamidementioning

confidence: 99%

Hepatoprotective Activity of Some Medicinal Plants: A Review

Hassan¹,

Veerakumar²,

Naseer³

et al. 2019

Int Res J Pharm

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Liver is an essential organ. Its plays a main function in metabolism and excretion of xenobiotics from the body. Moreover it is also coping with the metabolism and excretion of medicine and other xenobiotics from the frame there with the aid of supplying safety towards foreign substances by using detoxifying agents and putting them off. The most important capabilities of the liver are carbohydrate, protein, fat metabolism and detoxification, secretion of bile and storage of vitamins. In this examine, on line databases together with web of Science, Pub Med, Scopus, and Science Direct were looked for papers. Liver illnesses are first rate problem in worldwide. Liver damage or liver disorder is a major fitness troubles those demanding situations no longer most effective health care professionals but also the pharmaceutical industry and drug regulatory companies. Liver disease (Hepatic disease) is a term that impacts the cells, tissues, systems or factures of the liver. Liver cell harm resulting from various toxic chemical compounds (along with antibiotic , chemotherapeutic retailers, thioacetamide and paracetamol and so forth.), excessive alcohol intake and microbes are nicely studied. A number of natural tablets show promising hepatoprotective activities in acute and chronic liver damage. In medication the natural products play an essential role because of their protection, efficacy and price effectiveness. Medicinal plants might also function as vital source of doubtlessly useful for the development of effective therapy to fight several liver troubles. In this review we centred on the number of medicinal plants for protective effect in liver diseases.

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Compounds for Control of Orange Decays

Cited by 26 publications

References 0 publications

Assessing Chemical-Induced Liver Injury In Vivo From In Vitro Gene Expression Data in the Rat: The Case of Thioacetamide Toxicity

Assessing Chemical-Induced Liver Injury In Vivo From In Vitro Gene Expression Data in the Rat: The Case of Thioacetamide Toxicity

Co-administration of cyclosporine A alleviates thioacetamide-induced liver injury

Hepatoprotective Activity of Some Medicinal Plants: A Review

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