Comprehensive analysis of AP2/ERF gene members in Acer truncatum B. and the positive regulator role of AtruDREB28 in drought tolerance

Li, Jianbo; Guo, Wei; Meng, Hongyu; Zhao, Jinna; Zheng, Guoxing; Yuan, Weijie

doi:10.1016/j.indcrop.2023.116837

Cited by 9 publications

(4 citation statements)

References 42 publications

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“…Similar findings have been reported for the overexpression of ZmEREBP60 [42], OsERF115 [43], and OsERF71 [44]. In A. truncatum, AtruDREB28 was induced by drought stress and play positive role in drought stress resistance in our previous study [45]. Additionally, MYB genes also contribute to plant defenses against drought stress.…”

Section: Discussionsupporting

confidence: 88%

Transcriptional Regulation of the Acer truncatum B. Response to Drought and the Contribution of AtruNAC36 to Drought Tolerance

Guo

Zhao

et al. 2023

Antioxidants

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Drought stress is one of the major environmental factors severely restricting plant development and productivity. Acer truncatum B, which is an economically important tree species, is highly tolerant to drought conditions, but the underlying molecular regulatory mechanisms remain relatively unknown. In this study, A. truncatum seedlings underwent a drought treatment (water withheld for 0, 3, 7, and 12 days), after which they were re-watered for 5 days. Physiological indices were measured and a transcriptome sequencing analysis was performed to reveal drought response-related regulatory mechanisms. In comparison to the control, the drought treatment caused a significant increase in antioxidant enzyme activities, with levels rising up to seven times, and relative electrical conductivity from 14.5% to 78.4%, but the relative water content decreased from 88.3% to 23.4%; these indices recovered somewhat after the 5-day re-watering period. The RNA sequencing analysis identified 9126 differentially expressed genes (DEGs), which were primarily involved with abscisic acid responses, and mitogen-activated protein kinase signaling. These DEGs included 483 (5.29%) transcription factor genes from 53 families, including ERF, MYB, and NAC. A co-expression network analysis was conducted and three important modules were analyzed to identify hub genes, one of which (AtruNAC36) was examined to clarify its function. The AtruNAC36 protein was localized to the nucleus and had a C-terminal transactivation domain. Moreover, it bounded specifically to the NACRS element. The overexpression of AtruNAC36 in Arabidopsis thaliana resulted in increased drought tolerance by enhancing antioxidant enzyme activities. These findings provide important insights into the transcriptional regulation mediating the A. truncatum response to drought. Furthermore, AtruNAC36 may be relevant for breeding forest trees resistant to drought stress.

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Section: Discussionsupporting

confidence: 88%

Transcriptional Regulation of the Acer truncatum B. Response to Drought and the Contribution of AtruNAC36 to Drought Tolerance

Guo

Zhao

et al. 2023

Antioxidants

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“…Kong et al [ 38 ] proved that overexpression of PtoERF15 contributed to stem water potential maintenance in response to water deficit in Populus tomentosa , thus promoting drought tolerance. Similarly, Li et al [ 39 ] revealed that overexpression of AtruDREB28 increased tolerance to drought stress by enhancing reactive oxygen species-scavenging capability in Acer truncatum . Zhu et al [ 40 ] reported that CqERF24 overexpression in Arabidopis thaliana lines could enhance drought resistance through increased antioxidant enzyme activity and activated related stress genes, while silencing CqERF24 in quinoa decreased drought tolerance and overexpression of CqERF24 in quinoa calli enhanced resistance to mannitol.…”

Section: Discussionmentioning

confidence: 96%

Differentially Expressed Genes Identification of Kohlrabi Seedlings (Brassica oleracea var. caulorapa L.) under Polyethylene Glycol Osmotic Stress and AP2/ERF Transcription Factor Family Analysis

Bian,

Zhao,

Zhang

et al. 2024

Plants

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Osmotic stress is a condition in which plants do not get enough water due to changes in environmental factors. Plant response to osmotic stress is a complex process involving the interaction of different stress-sensitive mechanisms. Differentially expressed genes and response mechanisms of kohlrabi have not been reported under osmotic stress. A total of 196,642 unigenes and 33,040 differentially expressed unigenes were identified in kohlrabi seedlings under polyethylene glycol osmotic stress. AP2/ERF, NAC and eight other transcription factor family members with a high degree of interaction with CAT and SOD antioxidant enzyme activity were identified. Subsequently, 151 AP2/ERF genes were identified and analyzed. Twelve conserved motifs were searched and all AP2/ERF genes were clustered into four groups. A total of 149 AP2/ERF genes were randomly distributed on the chromosome, and relative expression level analysis showed that BocAP2/ERF genes of kohlrabi have obvious specificity in different tissues. This study lays a foundation for explaining the osmotic stress resistance mechanism of kohlrabi and provides a theoretical basis for the functional analysis of BocAP2/ERF transcription factor family members.

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“…These cis-elements play crucial roles in mediating the light response. Moreover, the close regulatory interaction of TFs, that is, bZIP with G-box (Hsieh et al, 2012), box (Zhang et al, 2010), ABRE (ACGTGG/TC) (Nakashima & Yamaguchi-Shinozaki, 2006), and MBS (Li, Guo, et al, 2023), have been thoroughly investigated in previous studies. Furthermore, the MYB (Joshi et al, 2016), WRKY (Mare et al, 2004), and DREB (Cui et al, 2011) family of TFs has been annotated in detail for drought stress tolerance mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…Genome‐wide identification studies have reported different TF(s) involved in recognizing the respective cis ‐elements under light and drought stress. The cis ‐elements reported in the drought stress response, that is, DRE (Liu et al, 2000 ), CATGTG (Tran et al, 2004 ), AATCA (Liu et al, 2022 ), GCC box (Zhang et al, 2010 ), ABRE (ACGTGG/TC) (Nakashima & Yamaguchi‐Shinozaki, 2006 ), and MBS (Li, Guo, et al, 2023 ), have been thoroughly investigated in previous studies. Furthermore, the MYB (Joshi et al, 2016 ), WRKY (Mare et al, 2004 ), and DREB (Cui et al, 2011 ) family of TFs has been annotated in detail for drought stress tolerance mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide characterization and expression analysis of GATA transcription factors under combination of light wavelengths and drought stress in potato

Aksoy,

Yavuz,

Yagiz

et al. 2024

Plant Direct

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GATA is one of the prominent transcription factor families conserved among many organisms in eukaryotes and has different biological roles in many pathways, particularly in light regulation in plants. Although GATA transcription factors (TFs) have been identified in different crop species, their roles in abiotic stress tolerance have not been studied in potato. In this study, we identified 32 GATA TFs in potato (Solanum tuberosum) by in silico analyses, and expression levels of selected six genes were investigated in drought‐tolerant (Sante) and sensitive (Agria) cultivars under light, drought, and combined (light + drought) stress conditions. According to the phylogenetic results, StGATA TFs were divided into four main groups (I, II, III, and IV) and different sub‐groups in I and II (eight and five, respectively). StGATA genes were uniformly localized to each chromosome with a conserved exon/intron structure. The presence of cis‐elements within the StGATA family further supported the possible involvement in abiotic stress tolerance and light response, tissue‐specific expression, and hormonal regulation. Additional PPI investigations showed that these networks, especially for Groups I, II, and IV, play a significant role in response to light and drought stress. Six StGATAs were chosen from these groups for expressional profiling, and their expression in both Sante and Agria was mainly downregulated under purple and red lights, drought, and combined stress (blue + drought and purple + drought). The interactomes of selected StGATAs, StGATA3, StGATA24, and StGATA29 were analyzed, and the accessions with GATA motifs were checked for expression. The results showed that the target proteins, cyclin‐P3‐1, SPX domain‐containing protein 1, mitochondrial calcium uniporter protein 2, mitogen‐activated protein kinase kinase kinase YODA, and splicing factor 3 B subunit 4‐like, mainly play a role in phytochrome‐mediated stomatal patterning, development, and activity. Understanding the interactions between drought stress and the light response mechanisms in potato plants is essential. It will eventually be possible to enhance potato resilience to climate change by manipulating the TFs that play a role in these pathways.

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Comprehensive analysis of AP2/ERF gene members in Acer truncatum B. and the positive regulator role of AtruDREB28 in drought tolerance

Cited by 9 publications

References 42 publications

Transcriptional Regulation of the Acer truncatum B. Response to Drought and the Contribution of AtruNAC36 to Drought Tolerance

Transcriptional Regulation of the Acer truncatum B. Response to Drought and the Contribution of AtruNAC36 to Drought Tolerance

Differentially Expressed Genes Identification of Kohlrabi Seedlings (Brassica oleracea var. caulorapa L.) under Polyethylene Glycol Osmotic Stress and AP2/ERF Transcription Factor Family Analysis

Genome‐wide characterization and expression analysis of GATA transcription factors under combination of light wavelengths and drought stress in potato

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