Comprehensive analysis of arachidonic acid metabolism-related genes in diagnosis and synovial immune in osteoarthritis: based on bulk and single-cell RNA sequencing data

Comprehensive Analysis of Sphingolipid Metabolism-Related Genes in Osteoarthritic Diagnosis and Synovial Immune Dysregulation

“…Similarly, Hannah et al [ 48 ] used single-cell analysis to highlight the significant role of the gene ZEB1, selected from single-cell transcriptome data of joint cartilage and meniscus, in the pathogenesis of OA. Previous research from our team has also indicated the involvement of genes within the arachidonic acid metabolism pathway in OA diagnosis and local inflammation within synovial tissues [ 23 ]. In this study, we found that the expression of SMP-related hub genes in synovium can serve as robust early markers for OA and are correlated with local inflammatory cell infiltration and levels of inflammatory factors, suggesting the tight correlation between the SMP-related hub genes and immune disorder in OA synovium.…”

“…However, the confirmation of OA depends on imaging data and patient symptoms [ 19 , 20 ], and timely diagnosis of OA is difficult due to lack of specific clinical manifestations. The effective recognition of OA is key to preventing the loss of articular cartilage and OA progression in joints [ 17 , 21 ], and numerous studies have unveiled early diagnostic biomarkers for OA [ 22 , 23 ], but the clinical utilization of these biomarkers remains a challenge, underscoring the need for further exploration of potential candidate markers for clinical application.…”

Single-Cell RNA Sequencing and Microarray Analysis Reveal the Role of Lipid-Metabolism-Related Genes and Cellular Immune Infiltration in Pre-Eclampsia and Identify Novel Biomarkers for Pre-Eclampsia

“…According to the GSEA results, the expression of PLA2G7 exhibited significant associations with pathway enrichment in pathways related to spliceosomes [ 49 ], minoacyl-tRNA biosynthesis, olfactory transduction, and drug metabolism–cytochrome P450. In addition, the cohorts with low PTGS2 expression demonstrated noteworthy enrichment in immune-related pathways [ 50 ], including Toll-like receptor signaling and Fc gamma R-mediated phagocytosis. In GSVA, the downregulation of PTGS2 expression was significantly associated with various pathways [ 51 ], including folate biosynthesis, dorsoventral axis formation, renal cell carcinoma, regulation of autophagy, glycosphingolipid biosynthesis (lacto and neolacto series), pantothenate and CoA biosynthesis, and O-glycan biosynthesis.…”

Exploring biomarkers associated with severity of knee osteoarthritis in Southern China using widely targeted metabolomics

“…In recent years, as research into the pathogenesis of OA has intensified, there is increasing evidence that OA is an inflammatory disease [44].KOA patients often suffer from synovitis, which is considered one of the causes of joint pain [45,46]. Synovial inflammation is accompanied by the release of pro-inflammatory mediators such as prostaglandin E2 (PGE2), nitric oxide (NO) [47,48].Study finds arachidonic acid metabolism-related genes strongly associated with synovitis [49]. In clinical practice, patients with severe KOA are often associated with more severe joint pain than those with mild KOA.…”