Comprehensive analysis of lncRNA and mRNA based on expression microarray profiling reveals different characteristics of osteoarthritis between Tibetan and Han patients

Abstract

Background Osteoarthritis (OA) is thought to be the most prevalent chronic joint disease, especially in Tibet of China. Here, we aimed to explore the integrative lncRNA and mRNA landscape between the OA patients of Tibet and Han. Methods The lncRNA and mRNA expression microarray profiling was performed by SurePrint G3 Human Gene Expression 8x60K v2 Microarray in articular cartilage samples from OA patients of Han nationality and Tibetans, followed by GO, KEGG, and trans-regulation and cis-regulation analysis of lncRNA and mRNA. Results We found a total of 117 lncRNAs and 297 mRNAs differently expressed in the cartilage tissues of Tibetans (n = 5) comparing with those of Chinese Han (n = 3), in which 49 lncRNAs and 158 mRNAs were upregulated, and 68 lncRNAs and 139 mRNAs were downregulated. GO and KEGG analysis showed that several unreported biological processes and signaling pathways were particularly identified. LncRNA-mRNA co-expression analysis revealed a remarkable lncRNA-mRNA relationship, in which OTOA may play a critical role in the different mechanisms of the OA progression between Tibetans and Chinese Han. Conclusion This study identified different lncRNA/mRNA expression profiling between OA patients of Tibetans and Han, which were involved in many characteristic biological processes and signaling pathways.

The emerging role of lncRNAs in osteoarthritis development and potential therapy

“…By comprehensive analysis of mRNA and lncRNA, Luo et al revealed different features of OA between Han population and Tibetan patients. Specifically, 117 lncRNAs (49 upregulated and 68 downregulated) and 297 mRNAs (158 upregulated and 139 downregulated) had differently expressed in the cartilage of Tibetans compared with those of Han patients (Luo et al, 2021). RNA-sequencing was performed to identify the changed expression of mRNA and lncRNAs in between 5 osteoarthritic synovium samples and 5 healthy tissues.…”

Z-DNA–Containing Long Terminal Repeats of Human Endogenous Retrovirus Families Provide Alternative Promoters for Human Functional Genes

“…Moreover, numerous lncRNAs are differentially expressed in human OA cartilage ( Abbasifard et al, 2020 ). LncRNA-mRNA co-expression analysis has revealed a remarkable relationship, wherein OTOA may play a critical role in the differential mechanisms of OA progression between Tibetan and Han Chinese populations ( Luo et al, 2021 ). LncRNA-derived LINC00652 can exert biological functions by co-expression with prognostic genes ( INSL3 , SNAP91 , and REN ) and lipid metabolism-related genes ( MIA2, APOA1 ).…”