Comprehensive analysis of maté (Ilex paraguariensis) compounds: Development of chemical strategies for matesaponin analysis by mass spectrometry

Souza, Lauro Mera de; Dartora, Nessana; Scoparo, Camila T.; Cipriani, Thales R.; Gorin, Philip A.J.; Iacomini, Marcello; Sassaki, Guilherme L.

doi:10.1016/j.chroma.2011.08.047

Cited by 38 publications

(36 citation statements)

References 28 publications

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“…In order to determine its chemical composition, the fraction HE-Bu (2 mg) was hydrolyzed in 0.5 mL of 45% formic acid (v/v), 14 h, 100°C [19]. The sample was dried under N 2 stream, then mixed in n -butanol-H 2 O (1 : 1, v/v), yielding two layers which were separated and evaporated.…”

Section: Methodsmentioning

confidence: 99%

“…The sample was dried under N 2 stream, then mixed in n -butanol-H 2 O (1 : 1, v/v), yielding two layers which were separated and evaporated. The organic layer was evaluated by liquid chromatography analysis, whereas the aqueous one was submitted to successive reduction in NaBH 4 and acetylation in pyridine-acetic anhydride (200 μ L; 1 : 1, v/v) to analyze the monosaccharide composition, by gas chromatography-mass spectrometry (GC-MS) [19]. …”

Section: Methodsmentioning

confidence: 99%

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Analysis of Flavonoids fromEugenia unifloraLeaves and Its Protective Effect against Murine Sepsis

Rattmann

Souza

Malquevicz-Paiva

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Analysis of Flavonoids fromEugenia unifloraLeaves and Its Protective Effect against Murine Sepsis

Rattmann

Souza

Malquevicz-Paiva

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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“…5 Isopropylidenation is a common derivatization used to block specific hydroxyl groups of monosaccharides, used in oligosaccharide synthesis [19]. In an analytical approach, isopropylidenation was used to improve the identification of saponins by mass spectrometry with different glycosylation patterns [20]. Isopropylidenation was also crucial to the correct identification of isomeric dicaffeoylquinic acids with potent antiulcer activity [21].…”

Section: Introductionmentioning

confidence: 99%

Differentiation of flavonol glucoside and galactoside isomers combining chemical isopropylidenation with liquid chromatography–mass spectrometry analysis

Souza

Dartora

Scoparo

et al. 2016

Journal of Chromatography A

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“…According to the literature, matesaponins in I. paraguariensis samples mainly consist of ursolic acid derivatives (22). + with two losses of 162 Da and one loss of 150 Da, corresponding to two glycosyl and one arabinosyl residues (23). Fragmentation associated with loss of sugar molecules is common, because matesaponins are glycosylated compounds.…”

Section: Discussionmentioning

confidence: 99%

<em>Ilex paraguariensis</em> Extracts Extend the Lifespan of <em>Drosophila melanogaster </em>Fed a High Fat Diet

Colpo¹,

Lima²,

Rosa³

et al. 2017

Preprint

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Studies have suggested that total energy intake and diet composition affect lifespan and ageing. A high-fat diet induces oxidative stress and affects the development of diseases. In contrast, antioxidants are capable of reducing its harmful effects. Yerba mate beverages are an important source of antioxidants, but there is scarce knowledge about their effects on suppressing fat accumulation. Here, we investigated the compounds present in yerba mate extracts and assessed their effects on Drosophila melanogaster given a high cholesterol diet. LS-ESI-MS analysis showed the presence of matesaponins, phenolic compounds and methylxanthines in all of the examined extracts. In Drosophila, under extract treatment conditions, the mean lifespan was significantly extended from 38 to 43 days, there was an increase in the ability to support induced stress and decrease in lipid peroxidation products. Moreover, yerba mate extracts recovered the glutathione S-transferases (GST) activity and reduced the cholesterol level. Taken together, our results support that extracts can extend lifespan by reducing the detrimental effect of a high-fat diet in D. melanogaster, and this outcome can be associated with the compound content in the extracts. This study improves the understanding of natural interventions that reduce stress-induced oxidative damage, which is fundamental in promoting healthy ageing.

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Comprehensive analysis of maté (Ilex paraguariensis) compounds: Development of chemical strategies for matesaponin analysis by mass spectrometry

Cited by 38 publications

References 28 publications

Analysis of Flavonoids fromEugenia unifloraLeaves and Its Protective Effect against Murine Sepsis

Analysis of Flavonoids fromEugenia unifloraLeaves and Its Protective Effect against Murine Sepsis

Differentiation of flavonol glucoside and galactoside isomers combining chemical isopropylidenation with liquid chromatography–mass spectrometry analysis

<em>Ilex paraguariensis</em> Extracts Extend the Lifespan of <em>Drosophila melanogaster </em>Fed a High Fat Diet

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