Comprehensive analysis of microRNA expression and target prediction in children with Nephrotic syndrome

Abstract

Background Nephrotic syndrome is one of the common cause among the kidney disease in children worldwide .It is characterised by the edema proteinuria, hypoalbuminemia and hypocholestromia. Recently many studies have emerged with the association of microRNAs playing an potential roles in many pathophysiological functions. MicroRNAs(miRNAs ) and RNA binding proteins (RBPs) are found to be two most important needed transcriptional regulators of gene expression as well as for the aberrant expression that tend to contribute to the development of the disease. It can reduce translation neither by translation repression through or MicroRNA cleavage. In this present study we have checked for the expression pattern of the targeted microRNAs miR-17-5P,miR-155p ,miR- 424 -5p ,miR-1 and 215-5p in the Children among Steroid Sensitive Nephrotic syndrome(SSNS) Steroid Resistance Nephrotic Syndrome (SRNS)along with the healthy individuals. Total RNA was isolated from the urine samples among the three groups(SSNS = 100,SRNS = 100 and healthy individuals100).The expression pattern for theses microRNAs was carried out using RT-PCR .Bioinformatics tools such as miRWalk, miR-Tar link were used in predicting targets for the microRNAs an online data bases and g profiler software is used which was also helpful in evaluating the targets based on the biological functions, Molecular functions and the pathways related to the selected microRNAs, along with that ROC analysis was also performed which was widely helpful in selecting the microRNAs that could be used as a potential biomarker as well as a therapeutic target. Among the five microRNAs miR- 1,miR-215, miR-17-5P, ,miR-155-5p & miR-424-5p.Two microRNAs miR-424-5p& miR-155p is found to be up regulated in the SRNS group.